##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AscI AseI AsnI AspI
AvrII BclI BfrI BglII BlnI
BsaBI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrGI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DrdI EcoO65I EcoRV EcoT22I
FbaI HincII HindII HpaI MamI
MfeI MluI MroI MunI NdeI
NruI NsiI NspV PacI PflFI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SexAI
SfuI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 3688 GGTACC G^GTACC
AgeI 5061 ACCGGT A^CCGGT
AhdI 453 GACCAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 5026 AGCGCT AGC^GCT
Asp718I 3688 GGTACC G^GTACC
AspEI 453 GACCAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 124 GGATCC G^GATCC
BbrPI 937 CACGTG CAC^GTG
BcgI 3589 GCATCGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 937 CACGTG [CT]AC^GT[GA]
BspHI 169 TCATGA T^CATGA
BsrDI 1334 GCAATG GCAATG(2/0)
BssHII 3468 GCGCGC G^CGCGC
DraI 821 TTTAAA TTT^AAA
Eam1105I 453 GACCAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5026 AGCGCT AGC^GCT
EcoRI 969 GAATTC G^AATTC
FseI 2220 GGCCGGCC GGCCGG^CC
KpnI 3688 GGTACC GGTAC^C
KspI 4159 CCGCGG CCGC^GG
NheI 1856 GCTAGC G^CTAGC
NotI 5159 GCGGCCGC GC^GGCCGC
PinAI 5061 ACCGGT A^CCGGT
PmaCI 937 CACGTG CAC^GTG
PmlI 937 CACGTG CAC^GTG
RcaI 169 TCATGA T^CATGA
SacII 4159 CCGCGG CCGC^GG
SapI 2450 GAAGAGC GCTCTTC(1/4)
SgrAI 5060 CACCGGTG C[GA]^CCGG[CT]G
TfiI 1763 GATTC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 969 GAATTC [GA]^AATT[CT]
3813 GAATTT
ApoI 969 GAATTC [GA]^AATT[CT]
3813 GAATTT
Asp700I 3654 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4223 GAACCTCTTC
AviII 865 TGCGCA TGC^GCA
4675 TGCGCA
BbeI 705 GGCGCC GGCGC^C
2474 GGCGCC
BsaI 963 GGTCTC GGTCTC(1/5)
2751 GGTCTC
BsmBI 3845 CGTCTC CGTCTC(1/5)
2182 GAGACG
FspI 865 TGCGCA TGC^GCA
4675 TGCGCA
HindIII 3344 AAGCTT A^AGCTT
4018 AAGCTT
KasI 705 GGCGCC G^GCGCC
2474 GGCGCC
MseI 687 TTAA T^TAA
822 TTAA
NarI 705 GGCGCC GG^CGCC
2474 GGCGCC
NcoI 1051 CCATGG C^CATGG
4660 CCATGG
PaeR7I 536 CTCGAG C^TCGAG
1101 CTCGAG
SfoI 705 GGCGCC GGC^GCC
2474 GGCGCC
SphI 1993 GCATGC GCATG^C
5018 GCATGC
StuI 1637 AGGCCT AGG^CCT
2540 AGGCCT
Tru9I 687 TTAA T^TAA
822 TTAA
Tsp509I 970 AATT ^AATT
3814 AATT
XhoI 536 CTCGAG C^TCGAG
1101 CTCGAG
XmnI 3654 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4223 GAACCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BciVI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
SmaI SmlI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI