##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspI AviII BcgI
BcnI BglI BlpI Bpu1102I BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BspDI BspEI BsrFI BssHII
BstAPI BstBI BstEII BstPI Bsu36I
CelII Cfr10I ClaI CpoI DraIII
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FseI FspI
HapII HpaII KpnI KspI MluI
MroI MspI NaeI NciI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI Psp1406I
PvuI RsrII SacII SalI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SwaI Tth111I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AcyI 153 GGCGCC G[GA]^CG[CT]C
AhdI 3490 GACATTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3490 GACATTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 4622 CTCGGG C^[CT]CG[GA]G
BalI 2006 TGGCCA TGG^CCA
BamHI 3094 GGATCC G^GATCC
BbeI 153 GGCGCC GGCGC^C
BbrPI 4200 CACGTG CAC^GTG
BbvCI 4596 CCTCAGC CCTCAGC(-5/-2)
BclI 3597 TGATCA T^GATCA
BpmI 3628 CTGGAG CTGGAG(16/14)
BsaBI 1804 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 153 GGCGCC G[GA]^CG[CT]C
BsmI 5460 GAATGC GAATGC(1/-1)
BsoBI 4622 CTCGGG C^[CT]CG[GA]G
BsrBI 89 CCGCTC CCGCTC(-3/-3)
BssSI 1720 CACGAG CACGAG(-5/-1)
Bst1107I 4205 GTATAC GTA^TAC
BstZ17I 4205 GTATAC GTA^TAC
CfoI 154 GCGC GCG^C
DrdI 2305 GACAACTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2006 TGGCCA [CT]^GGCC[GA]
Eam1105I 3490 GACATTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 3597 TGATCA T^GATCA
HaeII 153 GGCGCC [GA]GCGC^[CT]
HhaI 154 GCGC GCG^C
Hin1I 153 GGCGCC G[GA]^CG[CT]C
HinP1I 154 GCGC G^CGC
HincII 5229 GTTAAC GT[CT]^[GA]AC
HindII 5229 GTTAAC GT[CT]^[GA]AC
HpaI 5229 GTTAAC GTT^AAC
KasI 153 GGCGCC G^GCGCC
MamI 1804 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2006 TGGCCA TGG^CCA
MscI 2006 TGGCCA TGG^CCA
NarI 153 GGCGCC GG^CGCC
NheI 3067 GCTAGC G^CTAGC
PflMI 1124 CCAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4200 CACGTG CAC^GTG
PmlI 4200 CACGTG CAC^GTG
PpuMI 2581 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 5441 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2140 GAAGAGC GCTCTTC(1/4)
SbfI 4519 CCTGCAGG CCTGCA^GG
ScaI 2486 AGTACT AGT^ACT
SfoI 153 GGCGCC GGC^GCC
SphI 2266 GCATGC GCATG^C
Sse8387I 4519 CCTGCAGG CCTGCA^GG
TaqI 2328 TCGA T^CGA
TthHB8I 2328 TCGA T^CGA
Van91I 1124 CCAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 61 GTCTAC GT^[AC][GT]AC
4205 GTATAC
AccII 38 CGCG CG^CG
178 CGCG
AlwNI 3622 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
4106 CAGGGCCTG
AvaII 2582 GGACC G^G[AT]CC
5447 GGTCC
AvrII 2552 CCTAGG C^CTAGG
3187 CCTAGG
BanII 2805 GAGCTC G[GA]GC[CT]^C
5350 GAGCTC
BlnI 2552 CCTAGG C^CTAGG
3187 CCTAGG
BsaAI 3818 CACGTA [CT]AC^GT[GA]
4200 CACGTG
Bsp1407I 4234 TGTACA T^GTACA
4577 TGTACA
BspLU11I 4237 ACATGT A^CATGT
5208 ACATGT
BsrGI 4234 TGTACA T^GTACA
4577 TGTACA
BstUI 38 CGCG CG^CG
178 CGCG
BstXI 1797 CCAGGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2009 CCAGACAACTGG
EcoNI 396 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2585 CCTCTTCAAGG
MfeI 1289 CAATTG C^AATTG
2527 CAATTG
MunI 1289 CAATTG C^AATTG
2527 CAATTG
MvnI 38 CGCG CG^CG
178 CGCG
SacI 2805 GAGCTC GAGCT^C
5350 GAGCTC
SpeI 4318 ACTAGT A^CTAGT
5184 ACTAGT
SspBI 4234 TGTACA T^GTACA
4577 TGTACA
Tsp45I 198 GTCAC ^GT[GC]AC
3578 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI ApoI AseI AsnI
Asp700I AspHI BanI BbsI BbvI
BciVI BfaI BfrI BglII BmyI
BpuAI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EarI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HgaI HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NcoI NdeI NdeII NlaIII
NlaIV NspI PleI PshBI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StuI StyI TfiI Tru9I TseI
Tsp509I TspRI XbaI XhoII XmnI