##################################
# NO CUT ENZYMES (   60 enzymes) #
##################################

AatII       AccI        AclI        AcsI        AgeI        
ApoI        AscI        AseI        AsnI        Asp700I     
AspI        BbrPI       BsaBI       Bsp1407I    BspDI       
BspHI       BspLU11I    BsrGI       BssHII      Bst1107I    
BstBI       BstZ17I     ClaI        DraI        EcoRI       
EcoRV       FseI        HpaI        MamI        MfeI        
MluI        MunI        NdeI        NotI        NruI        
NspV        PacI        PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        SalI        SbfI        SexAI       
SfuI        SgrAI       SpeI        Sse8387I    SspBI       
SspI        SwaI        Tth111I     XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

Acc65I        448  GGTACC               G^GTACC
AflIII       3100  ACACGT               A^C[GA][CT]GT
AhdI         5082  GACACACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       5275  GTGCAC               G^TGCAC
ApaLI        5275  GTGCAC               G^TGCAC
Asp718I       448  GGTACC               G^GTACC
AspEI        5082  GACACACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        2499  TGCGCA               TGC^GCA
BamHI         214  GGATCC               G^GATCC
BbeI         1574  GGCGCC               GGCGC^C
BciVI        2617  GTATCC               GTATCC(6/5)
BglII        3173  AGATCT               A^GATCT
BsiWI        1419  CGTACG               C^GTACG
BssSI        2622  CACGAG               CACGAG(-5/-1)
BstEII       3115  GGTGACC              G^GT[ACGT]ACC
BstPI        3115  GGTGACC              G^GT[ACGT]ACC
CpoI          311  CGGACCG              CG^G[AT]CCG
Eam1105I     5082  GACACACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      3115  GGTGACC              G^GT[ACGT]ACC
EcoT22I      4265  ATGCAT               ATGCA^T
FspI         2499  TGCGCA               TGC^GCA
HindIII      3163  AAGCTT               A^AGCTT
KasI         1574  GGCGCC               G^GCGCC
KpnI          448  GGTACC               GGTAC^C
KspI          218  CCGCGG               CCGC^GG
NaeI          196  GCCGGC               GCC^GGC
NarI         1574  GGCGCC               GG^CGCC
NgoMIV        196  GCCGGC               G^CCGGC
NheI         2556  GCTAGC               G^CTAGC
NsiI         4265  ATGCAT               ATGCA^T
PaeR7I       5620  CTCGAG               C^TCGAG
RsrII         311  CGGACCG              CG^G[AT]CCG
SacII         218  CCGCGG               CCGC^GG
ScaI         1108  AGTACT               AGT^ACT
SfiI         2215  GGCCCCCCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1574  GGCGCC               GGC^GCC
SnaBI        1421  TACGTA               TAC^GTA
XhoI         5620  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccII          62  CGCG                 CG^CG
              219  CGCG                
AccIII       1071  TCCGGA               T^CCGGA
             5504  TCCGGA              
AflII        3166  CTTAAG               C^TTAAG
             3630  CTTAAG              
AvrII        4824  CCTAGG               C^CTAGG
             5822  CCTAGG              
BclI         3137  TGATCA               T^GATCA
             3902  TGATCA              
BfrI         3166  CTTAAG               C^TTAAG
             3630  CTTAAG              
BlnI         4824  CCTAGG               C^CTAGG
             5822  CCTAGG              
BsaWI        1071  TCCGGA               [AT]^CCGG[AT]
             5504  TCCGGA              
BseAI        1071  TCCGGA               T^CCGGA
             5504  TCCGGA              
BsiEI         122  CGGCCG               CG[GA][CT]^CG
              194  CGGCCG              
BspEI        1071  TCCGGA               T^CCGGA
             5504  TCCGGA              
BstUI          62  CGCG                 CG^CG
              219  CGCG                
DrdI         1516  GACGAGATGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5837  GACAGCCCTGTC        
EagI          122  CGGCCG               C^GGCCG
              194  CGGCCG              
EclXI         122  CGGCCG               C^GGCCG
              194  CGGCCG              
Eco52I        122  CGGCCG               C^GGCCG
              194  CGGCCG              
FbaI         3137  TGATCA               T^GATCA
             3902  TGATCA              
HincII       1041  GTCAAC               GT[CT]^[GA]AC
             2676  GTTGAC              
HindII       1041  GTCAAC               GT[CT]^[GA]AC
             2676  GTTGAC              
MroI         1071  TCCGGA               T^CCGGA
             5504  TCCGGA              
MseI         3167  TTAA                 T^TAA
             3631  TTAA                
MvnI           62  CGCG                 CG^CG
              219  CGCG                
SacI          979  GAGCTC               GAGCT^C
             1067  GAGCTC              
Tru9I        3167  TTAA                 T^TAA
             3631  TTAA                
Tsp509I       409  AATT                 ^AATT
              576  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (  135 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AlwNI       AocI        Aor51HI     ApaI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbsI        BbvCI       BbvI        BcgI        BcnI        
BfaI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspMI       BsrBI       BsrDI       
BsrFI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstXI       BstYI       Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
EarI        Eco47III    Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmaI        
SmlI        SphI        StuI        StyI        TaqI        
TfiI        TseI        Tsp45I      TspRI       TthHB8I     
Van91I      XcmI        XhoII       XmaCI       XmaI