################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AatII AccI AclI AcsI AgeI ApoI AscI AseI AsnI Asp700I AspI BbrPI BsaBI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII Bst1107I BstBI BstZ17I ClaI DraI EcoRI EcoRV FseI HpaI MamI MfeI MluI MunI NdeI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI SalI SbfI SexAI SfuI SgrAI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### Acc65I 448 GGTACC G^GTACC AflIII 3100 ACACGT A^C[GA][CT]GT AhdI 5082 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 5275 GTGCAC G^TGCAC ApaLI 5275 GTGCAC G^TGCAC Asp718I 448 GGTACC G^GTACC AspEI 5082 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 2499 TGCGCA TGC^GCA BamHI 214 GGATCC G^GATCC BbeI 1574 GGCGCC GGCGC^C BciVI 2617 GTATCC GTATCC(6/5) BglII 3173 AGATCT A^GATCT BsiWI 1419 CGTACG C^GTACG BssSI 2622 CACGAG CACGAG(-5/-1) BstEII 3115 GGTGACC G^GT[ACGT]ACC BstPI 3115 GGTGACC G^GT[ACGT]ACC CpoI 311 CGGACCG CG^G[AT]CCG Eam1105I 5082 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 3115 GGTGACC G^GT[ACGT]ACC EcoT22I 4265 ATGCAT ATGCA^T FspI 2499 TGCGCA TGC^GCA HindIII 3163 AAGCTT A^AGCTT KasI 1574 GGCGCC G^GCGCC KpnI 448 GGTACC GGTAC^C KspI 218 CCGCGG CCGC^GG NaeI 196 GCCGGC GCC^GGC NarI 1574 GGCGCC GG^CGCC NgoMIV 196 GCCGGC G^CCGGC NheI 2556 GCTAGC G^CTAGC NsiI 4265 ATGCAT ATGCA^T PaeR7I 5620 CTCGAG C^TCGAG RsrII 311 CGGACCG CG^G[AT]CCG SacII 218 CCGCGG CCGC^GG ScaI 1108 AGTACT AGT^ACT SfiI 2215 GGCCCCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1574 GGCGCC GGC^GCC SnaBI 1421 TACGTA TAC^GTA XhoI 5620 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 62 CGCG CG^CG 219 CGCG AccIII 1071 TCCGGA T^CCGGA 5504 TCCGGA AflII 3166 CTTAAG C^TTAAG 3630 CTTAAG AvrII 4824 CCTAGG C^CTAGG 5822 CCTAGG BclI 3137 TGATCA T^GATCA 3902 TGATCA BfrI 3166 CTTAAG C^TTAAG 3630 CTTAAG BlnI 4824 CCTAGG C^CTAGG 5822 CCTAGG BsaWI 1071 TCCGGA [AT]^CCGG[AT] 5504 TCCGGA BseAI 1071 TCCGGA T^CCGGA 5504 TCCGGA BsiEI 122 CGGCCG CG[GA][CT]^CG 194 CGGCCG BspEI 1071 TCCGGA T^CCGGA 5504 TCCGGA BstUI 62 CGCG CG^CG 219 CGCG DrdI 1516 GACGAGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5837 GACAGCCCTGTC EagI 122 CGGCCG C^GGCCG 194 CGGCCG EclXI 122 CGGCCG C^GGCCG 194 CGGCCG Eco52I 122 CGGCCG C^GGCCG 194 CGGCCG FbaI 3137 TGATCA T^GATCA 3902 TGATCA HincII 1041 GTCAAC GT[CT]^[GA]AC 2676 GTTGAC HindII 1041 GTCAAC GT[CT]^[GA]AC 2676 GTTGAC MroI 1071 TCCGGA T^CCGGA 5504 TCCGGA MseI 3167 TTAA T^TAA 3631 TTAA MvnI 62 CGCG CG^CG 219 CGCG SacI 979 GAGCTC GAGCT^C 1067 GAGCTC Tru9I 3167 TTAA T^TAA 3631 TTAA Tsp509I 409 AATT ^AATT 576 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 135 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI AocI Aor51HI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco47III Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SphI StuI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI