##################################
# NO CUT ENZYMES ( 44 enzymes) #
##################################
AgeI AscI Asp700I AspI AviII
AvrII BcgI BlnI BsiWI BssHII
Bst1107I BstBI BstZ17I DraIII EagI
EclXI Eco52I FseI FspI HpaI
KspI MluI NaeI NgoMIV NotI
NruI NspV PacI PflFI PinAI
PmeI PshAI PvuI SacII SbfI
SexAI SfuI SgrAI SnaBI SpeI
Sse8387I Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 3343 GACGTC GACGT^C
Acc65I 2208 GGTACC G^GTACC
AccII 3510 CGCG CG^CG
AclI 1096 AACGTT AA^CGTT
AflII 3255 CTTAAG C^TTAAG
Alw44I 1414 GTGCAC G^TGCAC
AocI 2608 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2387 AGCGCT AGC^GCT
ApaLI 1414 GTGCAC G^TGCAC
Asp718I 2208 GGTACC G^GTACC
BamHI 1267 GGATCC G^GATCC
BbrPI 5167 CACGTG CAC^GTG
BfrI 3255 CTTAAG C^TTAAG
BglII 4747 AGATCT A^GATCT
BlpI 3532 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3532 GCTAAGC GC^T[ACGT]AGC
BsaBI 5657 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 3485 GAGACG CGTCTC(1/5)
BspDI 3337 ATCGAT AT^CGAT
BsrBI 4341 CCGCTC CCGCTC(-3/-3)
BsrFI 4367 GCCGGT [GA]^CCGG[CT]
BstAPI 4824 GCAAAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 3510 CGCG CG^CG
Bsu36I 2608 CCTGAGG CC^T[ACGT]AGG
CelII 3532 GCTAAGC GC^T[ACGT]AGC
Cfr10I 4367 GCCGGT [GA]^CCGG[CT]
ClaI 3337 ATCGAT AT^CGAT
CpoI 1949 CGGACCG CG^G[AT]CCG
Eco47III 2387 AGCGCT AGC^GCT
Eco81I 2608 CCTGAGG CC^T[ACGT]AGG
HindIII 4188 AAGCTT A^AGCTT
KpnI 2208 GGTACC GGTAC^C
MamI 5657 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4593 CAATTG C^AATTG
MunI 4593 CAATTG C^AATTG
MvnI 3510 CGCG CG^CG
NdeI 1831 CATATG CA^TATG
PaeR7I 3757 CTCGAG C^TCGAG
PmaCI 5167 CACGTG CAC^GTG
PmlI 5167 CACGTG CAC^GTG
Psp1406I 1096 AACGTT AA^CGTT
RsrII 1949 CGGACCG CG^G[AT]CCG
SacI 1122 GAGCTC GAGCT^C
SalI 925 GTCGAC G^TCGAC
SapI 3304 GAAGAGC GCTCTTC(1/4)
ScaI 2762 AGTACT AGT^ACT
SfiI 2146 GGCCCTGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 2010 CCCGGG CCC^GGG
StuI 3615 AGGCCT AGG^CCT
SwaI 5355 ATTTAAAT ATTT^AAAT
XhoI 3757 CTCGAG C^TCGAG
XmaCI 2010 CCCGGG C^CCGGG
XmaI 2010 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 925 GTCGAC GT^[AC][GT]AC
4282 GTAGAC
AccIII 1651 TCCGGA T^CCGGA
5112 TCCGGA
AlwNI 572 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
1147 CAGTACCTG
BbvCI 2086 CCTCAGC CCTCAGC(-5/-2)
2282 CCTCAGC
BclI 2841 TGATCA T^GATCA
5513 TGATCA
BglI 1865 GCCACGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2147 GCCCTGGCGGC
BsaAI 2661 TACGTG [CT]AC^GT[GA]
5167 CACGTG
BsaI 2819 GAGACC GGTCTC(1/5)
3958 GAGACC
BseAI 1651 TCCGGA T^CCGGA
5112 TCCGGA
BsiEI 927 CGACCG CG[GA][CT]^CG
1713 CGACCG
BsmI 242 GAATGC GAATGC(1/-1)
4718 GCATTC
BspEI 1651 TCCGGA T^CCGGA
5112 TCCGGA
BspMI 731 ACCTGC ACCTGC(4/8)
3265 GCAGGT
DrdI 1287 GACCCCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2331 GACGCCACCGTC
EarI 949 GAAGAG CTCTTC(1/4)
3304 GAAGAG
EcoNI 1985 CCTACAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2173 CCTTACCCAGG
EcoRI 2795 GAATTC G^AATTC
4348 GAATTC
FbaI 2841 TGATCA T^GATCA
5513 TGATCA
Ksp632I 949 GAAGAG CTCTTC(1/4)
3304 GAAGAG
MroI 1651 TCCGGA T^CCGGA
5112 TCCGGA
NheI 766 GCTAGC G^CTAGC
4314 GCTAGC
PpuMI 1544 AGGACCC [GA]G^G[AT]CC[CT]
3918 GGGACCC
XcmI 15 CCAGATGCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4319 CCATGGCCAGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AhdI AluI AlwI ApaI ApoI
AseI AsnI AspEI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvI BciVI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspHI BspLU11I BsrDI BsrGI
BsrI BssKI BssSI BstEII BstF5I
BstNI BstPI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI Eam1105I EcoO109I
EcoO65I EcoRII EcoRV EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PshBI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI SphI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII