##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Aor51HI AscI Asp718I
AviII BamHI BfrI BsaWI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BsrDI BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRV FseI FspI HindIII KpnI
MfeI MluI MroI MunI NaeI
NgoMIV NheI NotI NruI NspV
PacI PinAI PmeI PshAI Psp1406I
PvuI RsrII SalI SapI SfiI
SfuI SgrAI SnaBI SphI SspBI
SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AhdI 240 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 267 GTGCAC G^TGCAC
ApaLI 267 GTGCAC G^TGCAC
AseI 1936 ATTAAT AT^TAAT
AsnI 1936 ATTAAT AT^TAAT
Asp700I 3562 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 240 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 3662 GGCGCC GGCGC^C
BbrPI 4208 CACGTG CAC^GTG
BciVI 1672 GGATAC GTATCC(6/5)
BclI 2909 TGATCA T^GATCA
BlpI 3931 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3931 GCTCAGC GC^T[ACGT]AGC
BsaAI 4208 CACGTG [CT]AC^GT[GA]
BsmBI 4972 CGTCTC CGTCTC(1/5)
BsrFI 2801 ACCGGC [GA]^CCGG[CT]
BssHII 5015 GCGCGC G^CGCGC
BssSI 4767 CACGAG CACGAG(-5/-1)
CelII 3931 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2801 ACCGGC [GA]^CCGG[CT]
DraIII 793 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 240 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 2909 TGATCA T^GATCA
HpaI 4495 GTTAAC GTT^AAC
KasI 3662 GGCGCC G^GCGCC
KspI 4867 CCGCGG CCGC^GG
NarI 3662 GGCGCC GG^CGCC
PaeR7I 724 CTCGAG C^TCGAG
PmaCI 4208 CACGTG CAC^GTG
PmlI 4208 CACGTG CAC^GTG
PshBI 1936 ATTAAT AT^TAAT
SacII 4867 CCGCGG CCGC^GG
SbfI 3755 CCTGCAGG CCTGCA^GG
SexAI 2492 ACCAGGT A^CC[AT]GGT
SfoI 3662 GGCGCC GGC^GCC
SmaI 2887 CCCGGG CCC^GGG
SpeI 3296 ACTAGT A^CTAGT
Sse8387I 3755 CCTGCAGG CCTGCA^GG
StuI 3405 AGGCCT AGG^CCT
XhoI 724 CTCGAG C^TCGAG
XmaCI 2887 CCCGGG C^CCGGG
XmaI 2887 CCCGGG C^CCGGG
XmnI 3562 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 650 ACATGT A^C[GA][CT]GT
4569 ACATGT
AspI 2319 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2867 GACAGGGTC
AvrII 765 CCTAGG C^CTAGG
2069 CCTAGG
BcgI 1750 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5049 GCAGGAGAATCG
BglII 825 AGATCT A^GATCT
2090 AGATCT
BlnI 765 CCTAGG C^CTAGG
2069 CCTAGG
BsaBI 847 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3701 GATGCAAATC
BspLU11I 650 ACATGT A^CATGT
4569 ACATGT
DraI 2832 TTTAAA TTT^AAA
4842 TTTAAA
EarI 1283 CTCTTC CTCTTC(1/4)
4517 GAAGAG
EcoRI 113 GAATTC G^AATTC
373 GAATTC
EcoT22I 1242 ATGCAT ATGCA^T
4650 ATGCAT
HgaI 197 GACGC GACGC(5/10)
4551 GACGC
HincII 3433 GTTGAC GT[CT]^[GA]AC
4495 GTTAAC
HindII 3433 GTTGAC GT[CT]^[GA]AC
4495 GTTAAC
Ksp632I 1283 CTCTTC CTCTTC(1/4)
4517 GAAGAG
MamI 847 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3701 GATGCAAATC
NdeI 1418 CATATG CA^TATG
3424 CATATG
NsiI 1242 ATGCAT ATGCA^T
4650 ATGCAT
PflFI 2319 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2867 GACAGGGTC
PflMI 2115 CCAGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3402 CCAAGGCCTGG
SacI 727 GAGCTC GAGCT^C
2277 GAGCTC
ScaI 1912 AGTACT AGT^ACT
3101 AGTACT
Tth111I 2319 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2867 GACAGGGTC
Van91I 2115 CCAGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3402 CCAAGGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspMI BsrBI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII