################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI Aor51HI AscI Asp718I AviII BamHI BfrI BsaWI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BsrDI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI HindIII KpnI MfeI MluI MroI MunI NaeI NgoMIV NheI NotI NruI NspV PacI PinAI PmeI PshAI Psp1406I PvuI RsrII SalI SapI SfiI SfuI SgrAI SnaBI SphI SspBI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AhdI 240 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 267 GTGCAC G^TGCAC ApaLI 267 GTGCAC G^TGCAC AseI 1936 ATTAAT AT^TAAT AsnI 1936 ATTAAT AT^TAAT Asp700I 3562 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 240 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 3662 GGCGCC GGCGC^C BbrPI 4208 CACGTG CAC^GTG BciVI 1672 GGATAC GTATCC(6/5) BclI 2909 TGATCA T^GATCA BlpI 3931 GCTCAGC GC^T[ACGT]AGC Bpu1102I 3931 GCTCAGC GC^T[ACGT]AGC BsaAI 4208 CACGTG [CT]AC^GT[GA] BsmBI 4972 CGTCTC CGTCTC(1/5) BsrFI 2801 ACCGGC [GA]^CCGG[CT] BssHII 5015 GCGCGC G^CGCGC BssSI 4767 CACGAG CACGAG(-5/-1) CelII 3931 GCTCAGC GC^T[ACGT]AGC Cfr10I 2801 ACCGGC [GA]^CCGG[CT] DraIII 793 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 240 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FbaI 2909 TGATCA T^GATCA HpaI 4495 GTTAAC GTT^AAC KasI 3662 GGCGCC G^GCGCC KspI 4867 CCGCGG CCGC^GG NarI 3662 GGCGCC GG^CGCC PaeR7I 724 CTCGAG C^TCGAG PmaCI 4208 CACGTG CAC^GTG PmlI 4208 CACGTG CAC^GTG PshBI 1936 ATTAAT AT^TAAT SacII 4867 CCGCGG CCGC^GG SbfI 3755 CCTGCAGG CCTGCA^GG SexAI 2492 ACCAGGT A^CC[AT]GGT SfoI 3662 GGCGCC GGC^GCC SmaI 2887 CCCGGG CCC^GGG SpeI 3296 ACTAGT A^CTAGT Sse8387I 3755 CCTGCAGG CCTGCA^GG StuI 3405 AGGCCT AGG^CCT XhoI 724 CTCGAG C^TCGAG XmaCI 2887 CCCGGG C^CCGGG XmaI 2887 CCCGGG C^CCGGG XmnI 3562 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AflIII 650 ACATGT A^C[GA][CT]GT 4569 ACATGT AspI 2319 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2867 GACAGGGTC AvrII 765 CCTAGG C^CTAGG 2069 CCTAGG BcgI 1750 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 5049 GCAGGAGAATCG BglII 825 AGATCT A^GATCT 2090 AGATCT BlnI 765 CCTAGG C^CTAGG 2069 CCTAGG BsaBI 847 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3701 GATGCAAATC BspLU11I 650 ACATGT A^CATGT 4569 ACATGT DraI 2832 TTTAAA TTT^AAA 4842 TTTAAA EarI 1283 CTCTTC CTCTTC(1/4) 4517 GAAGAG EcoRI 113 GAATTC G^AATTC 373 GAATTC EcoT22I 1242 ATGCAT ATGCA^T 4650 ATGCAT HgaI 197 GACGC GACGC(5/10) 4551 GACGC HincII 3433 GTTGAC GT[CT]^[GA]AC 4495 GTTAAC HindII 3433 GTTGAC GT[CT]^[GA]AC 4495 GTTAAC Ksp632I 1283 CTCTTC CTCTTC(1/4) 4517 GAAGAG MamI 847 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3701 GATGCAAATC NdeI 1418 CATATG CA^TATG 3424 CATATG NsiI 1242 ATGCAT ATGCA^T 4650 ATGCAT PflFI 2319 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2867 GACAGGGTC PflMI 2115 CCAGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3402 CCAAGGCCTGG SacI 727 GAGCTC GAGCT^C 2277 GAGCTC ScaI 1912 AGTACT AGT^ACT 3101 AGTACT Tth111I 2319 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2867 GACAGGGTC Van91I 2115 CCAGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3402 CCAAGGCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspMI BsrBI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII