##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccII AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp700I AspEI AviII BamHI
BbeI BcgI BglI BlpI Bpu1102I
BsaBI BseAI BsiEI BsiWI BspDI
BspEI BspHI BsrFI BssHII Bst1107I
BstEII BstPI BstUI BstZ17I CelII
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FseI FspI
HindIII KasI KspI MamI MfeI
MluI MroI MspA1I MunI MvnI
NaeI NarI NgoMIV NotI NruI
NsiI PacI PflMI PinAI PmeI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SwaI Van91I XcmI
XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 1763 GGTACC G^GTACC
AcyI 1925 GACGCC G[GA]^CG[CT]C
AflII 3320 CTTAAG C^TTAAG
AocI 5520 CCTCAGG CC^T[ACGT]AGG
Asp718I 1763 GGTACC G^GTACC
AspHI 3451 GAGCTC G[AT]GC[AT]^C
AvrII 4674 CCTAGG C^CTAGG
BalI 477 TGGCCA TGG^CCA
BanI 1763 GGTACC G^G[CT][GA]CC
BciVI 2313 GTATCC GTATCC(6/5)
BfrI 3320 CTTAAG C^TTAAG
BlnI 4674 CCTAGG C^CTAGG
BsaHI 1925 GACGCC G[GA]^CG[CT]C
BsaWI 2544 ACCGGA [AT]^CCGG[AT]
BsiHKAI 3451 GAGCTC G[AT]GC[AT]^C
BsmBI 2364 GAGACG CGTCTC(1/5)
BsmI 3275 GAATGC GAATGC(1/-1)
BstAPI 187 GCAAGGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 891 TTCGAA TT^CGAA
Bsu36I 5520 CCTCAGG CC^T[ACGT]AGG
CfoI 3513 GCGC GCG^C
DsaI 3587 CCATGG C^C[GA][CT]GG
EaeI 477 TGGCCA [CT]^GGCC[GA]
Eco81I 5520 CCTCAGG CC^T[ACGT]AGG
EcoNI 2495 CCTAGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 3512 AGCGCC [GA]GCGC^[CT]
HgaI 1925 GACGC GACGC(5/10)
HhaI 3513 GCGC GCG^C
Hin1I 1925 GACGCC G[GA]^CG[CT]C
HinP1I 3513 GCGC G^CGC
KpnI 1763 GGTACC GGTAC^C
MluNI 477 TGGCCA TGG^CCA
MscI 477 TGGCCA TGG^CCA
NcoI 3587 CCATGG C^CATGG
NheI 2903 GCTAGC G^CTAGC
NspV 891 TTCGAA TT^CGAA
PaeR7I 4889 CTCGAG C^TCGAG
PshAI 4902 GACTACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1251 CTGCAG CTGCA^G
SacI 3451 GAGCTC GAGCT^C
SapI 1819 GAAGAGC GCTCTTC(1/4)
SfuI 891 TTCGAA TT^CGAA
SmaI 363 CCCGGG CCC^GGG
XhoI 4889 CTCGAG C^TCGAG
XmaCI 363 CCCGGG C^CCGGG
XmaI 363 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 4588 GTAGAC GT^[AC][GT]AC
5203 GTAGAC
AlwNI 1210 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
4062 CAGGTCCTG
AspI 4610 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4787 GACAGGGTC
AvaI 363 CCCGGG C^[CT]CG[GA]G
4889 CTCGAG
BbrPI 42 CACGTG CAC^GTG
1143 CACGTG
BclI 989 TGATCA T^GATCA
4829 TGATCA
BglII 2612 AGATCT A^GATCT
3073 AGATCT
BpmI 336 CTGGAG CTGGAG(16/14)
632 CTGGAG
BsaAI 42 CACGTG [CT]AC^GT[GA]
1143 CACGTG
BsaI 3156 GAGACC GGTCTC(1/5)
4972 GGTCTC
BsoBI 363 CCCGGG C^[CT]CG[GA]G
4889 CTCGAG
BspMI 3466 ACCTGC ACCTGC(4/8)
1326 GCAGGT
BsrBI 3197 GAGCGG CCGCTC(-3/-3)
4575 GAGCGG
BsrDI 1893 GCAATG GCAATG(2/0)
3205 GCAATG
BssSI 2740 CACGAG CACGAG(-5/-1)
4744 CTCGTG
EcoRI 787 GAATTC G^AATTC
1109 GAATTC
FbaI 989 TGATCA T^GATCA
4829 TGATCA
HpaI 114 GTTAAC GTT^AAC
5612 GTTAAC
PflFI 4610 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4787 GACAGGGTC
PmaCI 42 CACGTG CAC^GTG
1143 CACGTG
PmlI 42 CACGTG CAC^GTG
1143 CACGTG
StuI 414 AGGCCT AGG^CCT
5517 AGGCCT
Tth111I 4610 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4787 GACAGGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI AvaII
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpuAI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspLU11I BsrGI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NciI
NdeI NdeII NlaIII NlaIV NspI
PleI PpuMI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoII