##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AccIII AclI AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI AspI
AviII AvrII BbeI BcgI BclI
BlnI BseAI BsiWI BsmI BspDI
BspEI BsrDI Bst1107I BstBI BstZ17I
ClaI CpoI DraI Eco47III FbaI
FseI FspI HpaI KasI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI Tth111I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 322 GACGTC GACGT^C
AcyI 322 GACGTC G[GA]^CG[CT]C
Asp700I 2857 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 10 GGATCC G^GATCC
BbrPI 2681 CACGTG CAC^GTG
BglII 2835 AGATCT A^GATCT
BsaAI 2681 CACGTG [CT]AC^GT[GA]
BsaHI 322 GACGTC G[GA]^CG[CT]C
BsaWI 779 ACCGGA [AT]^CCGG[AT]
BsmBI 340 GAGACG CGTCTC(1/5)
Bsp1407I 2844 TGTACA T^GTACA
BspLU11I 1794 ACATGT A^CATGT
BsrFI 548 ACCGGC [GA]^CCGG[CT]
BsrGI 2844 TGTACA T^GTACA
BssHII 28 GCGCGC G^CGCGC
BssSI 1517 CTCGTG CACGAG(-5/-1)
BstEII 4038 GGTCACC G^GT[ACGT]ACC
BstPI 4038 GGTCACC G^GT[ACGT]ACC
Cfr10I 548 ACCGGC [GA]^CCGG[CT]
DrdI 2890 GACCCAAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2086 CCTTCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4038 GGTCACC G^GT[ACGT]ACC
EcoRI 1317 GAATTC G^AATTC
EcoRV 2377 GATATC GAT^ATC
EcoT22I 4854 ATGCAT ATGCA^T
HaeII 2569 AGCGCC [GA]GCGC^[CT]
Hin1I 322 GACGTC G[GA]^CG[CT]C
KspI 44 CCGCGG CCGC^GG
NdeI 1078 CATATG CA^TATG
NsiI 4854 ATGCAT ATGCA^T
PmaCI 2681 CACGTG CAC^GTG
PmlI 2681 CACGTG CAC^GTG
SacII 44 CCGCGG CCGC^GG
SalI 1631 GTCGAC G^TCGAC
SspBI 2844 TGTACA T^GTACA
SspI 4796 AATATT AAT^ATT
XbaI 3272 TCTAGA T^CTAGA
XmnI 2857 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 3739 GGTACC G^GTACC
4172 GGTACC
AccI 1631 GTCGAC GT^[AC][GT]AC
4916 GTCTAC
AflII 2875 CTTAAG C^TTAAG
4483 CTTAAG
AflIII 1794 ACATGT A^C[GA][CT]GT
2682 ACGTGT
AhdI 702 GACCTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3673 GACTTTTTGTC
AocI 1670 CCTCAGG CC^T[ACGT]AGG
4580 CCTGAGG
Asp718I 3739 GGTACC G^GTACC
4172 GGTACC
AspEI 702 GACCTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3673 GACTTTTTGTC
BciVI 660 GGATAC GTATCC(6/5)
3348 GTATCC
BfrI 2875 CTTAAG C^TTAAG
4483 CTTAAG
BlpI 2670 GCTGAGC GC^T[ACGT]AGC
4968 GCTGAGC
Bpu1102I 2670 GCTGAGC GC^T[ACGT]AGC
4968 GCTGAGC
BsaBI 918 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1405 GATGACAATC
BspHI 3667 TCATGA T^CATGA
4477 TCATGA
BsrBI 1621 GAGCGG CCGCTC(-3/-3)
4971 GAGCGG
Bsu36I 1670 CCTCAGG CC^T[ACGT]AGG
4580 CCTGAGG
CelII 2670 GCTGAGC GC^T[ACGT]AGC
4968 GCTGAGC
DraIII 2261 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2467 CACTTCGTG
EagI 15 CGGCCG C^GGCCG
33 CGGCCG
Eam1105I 702 GACCTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3673 GACTTTTTGTC
EclXI 15 CGGCCG C^GGCCG
33 CGGCCG
Eco52I 15 CGGCCG C^GGCCG
33 CGGCCG
Eco81I 1670 CCTCAGG CC^T[ACGT]AGG
4580 CCTGAGG
HgaI 143 GACGC GACGC(5/10)
747 GACGC
HindIII 4430 AAGCTT A^AGCTT
4747 AAGCTT
KpnI 3739 GGTACC GGTAC^C
4172 GGTACC
MamI 918 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1405 GATGACAATC
NcoI 1343 CCATGG C^CATGG
3999 CCATGG
PaeR7I 1072 CTCGAG C^TCGAG
1992 CTCGAG
PshAI 1290 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3038 GACTGGAGTC
RcaI 3667 TCATGA T^CATGA
4477 TCATGA
SapI 162 GCTCTTC GCTCTTC(1/4)
1885 GCTCTTC
SexAI 197 ACCTGGT A^CC[AT]GGT
4034 ACCAGGT
SphI 57 GCATGC GCATG^C
272 GCATGC
XhoI 1072 CTCGAG C^TCGAG
1992 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII