##################################
# NO CUT ENZYMES (   42 enzymes) #
##################################

Acc65I      AclI        Aor51HI     ApaI        Asp718I     
AspI        AviII       AvrII       BlnI        BsiEI       
BsiWI       BsrBI       BstEII      BstPI       CpoI        
DrdI        EagI        EclXI       Eco47III    Eco52I      
EcoO65I     FseI        FspI        HpaI        KpnI        
MluI        NdeI        NotI        NruI        PacI        
PflFI       PmeI        Psp1406I    PvuI        RsrII       
SbfI        SexAI       SfiI        SnaBI       SpeI        
Sse8387I    Tth111I     


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccIII       2955  TCCGGA               T^CCGGA
AflII        7331  CTTAAG               C^TTAAG
AgeI         2033  ACCGGT               A^CCGGT
AhdI          207  GACGGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       3203  GTGCAC               G^TGCAC
AocI         2584  CCTCAGG              CC^T[ACGT]AGG
ApaLI        3203  GTGCAC               G^TGCAC
AscI           61  GGCGCGCC             GG^CGCGCC
AspEI         207  GACGGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI         2789  GGCGCC               GGCGC^C
BbrPI        2709  CACGTG               CAC^GTG
BfrI         7331  CTTAAG               C^TTAAG
BlpI         3377  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     3377  GCTGAGC              GC^T[ACGT]AGC
BsaBI        4972  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        2955  TCCGGA               T^CCGGA
BsmBI        4962  GAGACG               CGTCTC(1/5)
Bsp1407I     1008  TGTACA               T^GTACA
BspEI        2955  TCCGGA               T^CCGGA
BspLU11I     7676  ACATGT               A^CATGT
BsrGI        1008  TGTACA               T^GTACA
BssSI        4631  CTCGTG               CACGAG(-5/-1)
BstBI        6422  TTCGAA               TT^CGAA
Bsu36I       2584  CCTCAGG              CC^T[ACGT]AGG
CelII        3377  GCTGAGC              GC^T[ACGT]AGC
Eam1105I      207  GACGGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       2584  CCTCAGG              CC^T[ACGT]AGG
EcoT22I      5685  ATGCAT               ATGCA^T
HaeII        2789  GGCGCC               [GA]GCGC^[CT]
KasI         2789  GGCGCC               G^GCGCC
KspI         2816  CCGCGG               CCGC^GG
MamI         4972  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         2955  TCCGGA               T^CCGGA
NaeI          243  GCCGGC               GCC^GGC
NarI         2789  GGCGCC               GG^CGCC
NgoMIV        243  GCCGGC               G^CCGGC
NheI          189  GCTAGC               G^CTAGC
NsiI         5685  ATGCAT               ATGCA^T
NspV         6422  TTCGAA               TT^CGAA
PaeR7I       7359  CTCGAG               C^TCGAG
PinAI        2033  ACCGGT               A^CCGGT
PmaCI        2709  CACGTG               CAC^GTG
PmlI         2709  CACGTG               CAC^GTG
PshAI        4722  GACTAATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII        2816  CCGCGG               CCGC^GG
ScaI         2087  AGTACT               AGT^ACT
SfoI         2789  GGCGCC               GGC^GCC
SfuI         6422  TTCGAA               TT^CGAA
SgrAI         242  CGCCGGCG             C[GA]^CCGG[CT]G
SphI         6327  GCATGC               GCATG^C
SspBI        1008  TGTACA               T^GTACA
StuI         3144  AGGCCT               AGG^CCT
SwaI         6105  ATTTAAAT             ATTT^AAAT
XbaI          422  TCTAGA               T^CTAGA
XhoI         7359  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AatII        4123  GACGTC               GACGT^C
             6317  GACGTC              
AflIII       2708  ACACGT               A^C[GA][CT]GT
             7676  ACATGT              
AseI         4010  ATTAAT               AT^TAAT
             6728  ATTAAT              
AsnI         4010  ATTAAT               AT^TAAT
             6728  ATTAAT              
BamHI        2099  GGATCC               G^GATCC
             6232  GGATCC              
BsaAI        2709  CACGTG               [CT]AC^GT[GA]
             3179  TACGTG              
BspDI        2201  ATCGAT               AT^CGAT
             5083  ATCGAT              
BsrFI         243  GCCGGC               [GA]^CCGG[CT]
             2033  ACCGGT              
BssHII         62  GCGCGC               G^CGCGC
              200  GCGCGC              
Bst1107I     1654  GTATAC               GTA^TAC
             3464  GTATAC              
BstZ17I      1654  GTATAC               GTA^TAC
             3464  GTATAC              
Cfr10I        243  GCCGGC               [GA]^CCGG[CT]
             2033  ACCGGT              
ClaI         2201  ATCGAT               AT^CGAT
             5083  ATCGAT              
EcoNI        3214  CCTGCTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3721  CCTTTATCAGG         
MfeI         1671  CAATTG               C^AATTG
             4410  CAATTG              
MunI         1671  CAATTG               C^AATTG
             4410  CAATTG              
PshBI        4010  ATTAAT               AT^TAAT
             6728  ATTAAT              
SalI         2291  GTCGAC               G^TCGAC
             6314  GTCGAC              
SapI         4074  GAAGAGC              GCTCTTC(1/4)
             3625  GCTCTTC             


#########################################
# 3 OR MORE CUT ENZYMES (  142 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AluI        AlwI        AlwNI       ApoI        
Asp700I     AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbsI        BbvCI       BbvI        
BcgI        BciVI       BclI        BcnI        BfaI        
BglI        BglII       BmyI        BpmI        BpuAI       
BsaHI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspHI       
BspMI       BsrDI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       DraIII      DsaI        EaeI        
EarI        EcoO109I    EcoRI       EcoRII      EcoRV       
EcoT14I     FauI        FbaI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
PvuII       RcaI        RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmaI        
SmlI        SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoII       XmaCI       
XmaI        XmnI