################################## # NO CUT ENZYMES ( 42 enzymes) # ################################## Acc65I AclI Aor51HI ApaI Asp718I AspI AviII AvrII BlnI BsiEI BsiWI BsrBI BstEII BstPI CpoI DrdI EagI EclXI Eco47III Eco52I EcoO65I FseI FspI HpaI KpnI MluI NdeI NotI NruI PacI PflFI PmeI Psp1406I PvuI RsrII SbfI SexAI SfiI SnaBI SpeI Sse8387I Tth111I ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccIII 2955 TCCGGA T^CCGGA AflII 7331 CTTAAG C^TTAAG AgeI 2033 ACCGGT A^CCGGT AhdI 207 GACGGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3203 GTGCAC G^TGCAC AocI 2584 CCTCAGG CC^T[ACGT]AGG ApaLI 3203 GTGCAC G^TGCAC AscI 61 GGCGCGCC GG^CGCGCC AspEI 207 GACGGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 2789 GGCGCC GGCGC^C BbrPI 2709 CACGTG CAC^GTG BfrI 7331 CTTAAG C^TTAAG BlpI 3377 GCTGAGC GC^T[ACGT]AGC Bpu1102I 3377 GCTGAGC GC^T[ACGT]AGC BsaBI 4972 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 2955 TCCGGA T^CCGGA BsmBI 4962 GAGACG CGTCTC(1/5) Bsp1407I 1008 TGTACA T^GTACA BspEI 2955 TCCGGA T^CCGGA BspLU11I 7676 ACATGT A^CATGT BsrGI 1008 TGTACA T^GTACA BssSI 4631 CTCGTG CACGAG(-5/-1) BstBI 6422 TTCGAA TT^CGAA Bsu36I 2584 CCTCAGG CC^T[ACGT]AGG CelII 3377 GCTGAGC GC^T[ACGT]AGC Eam1105I 207 GACGGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2584 CCTCAGG CC^T[ACGT]AGG EcoT22I 5685 ATGCAT ATGCA^T HaeII 2789 GGCGCC [GA]GCGC^[CT] KasI 2789 GGCGCC G^GCGCC KspI 2816 CCGCGG CCGC^GG MamI 4972 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2955 TCCGGA T^CCGGA NaeI 243 GCCGGC GCC^GGC NarI 2789 GGCGCC GG^CGCC NgoMIV 243 GCCGGC G^CCGGC NheI 189 GCTAGC G^CTAGC NsiI 5685 ATGCAT ATGCA^T NspV 6422 TTCGAA TT^CGAA PaeR7I 7359 CTCGAG C^TCGAG PinAI 2033 ACCGGT A^CCGGT PmaCI 2709 CACGTG CAC^GTG PmlI 2709 CACGTG CAC^GTG PshAI 4722 GACTAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 2816 CCGCGG CCGC^GG ScaI 2087 AGTACT AGT^ACT SfoI 2789 GGCGCC GGC^GCC SfuI 6422 TTCGAA TT^CGAA SgrAI 242 CGCCGGCG C[GA]^CCGG[CT]G SphI 6327 GCATGC GCATG^C SspBI 1008 TGTACA T^GTACA StuI 3144 AGGCCT AGG^CCT SwaI 6105 ATTTAAAT ATTT^AAAT XbaI 422 TCTAGA T^CTAGA XhoI 7359 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AatII 4123 GACGTC GACGT^C 6317 GACGTC AflIII 2708 ACACGT A^C[GA][CT]GT 7676 ACATGT AseI 4010 ATTAAT AT^TAAT 6728 ATTAAT AsnI 4010 ATTAAT AT^TAAT 6728 ATTAAT BamHI 2099 GGATCC G^GATCC 6232 GGATCC BsaAI 2709 CACGTG [CT]AC^GT[GA] 3179 TACGTG BspDI 2201 ATCGAT AT^CGAT 5083 ATCGAT BsrFI 243 GCCGGC [GA]^CCGG[CT] 2033 ACCGGT BssHII 62 GCGCGC G^CGCGC 200 GCGCGC Bst1107I 1654 GTATAC GTA^TAC 3464 GTATAC BstZ17I 1654 GTATAC GTA^TAC 3464 GTATAC Cfr10I 243 GCCGGC [GA]^CCGG[CT] 2033 ACCGGT ClaI 2201 ATCGAT AT^CGAT 5083 ATCGAT EcoNI 3214 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3721 CCTTTATCAGG MfeI 1671 CAATTG C^AATTG 4410 CAATTG MunI 1671 CAATTG C^AATTG 4410 CAATTG PshBI 4010 ATTAAT AT^TAAT 6728 ATTAAT SalI 2291 GTCGAC G^TCGAC 6314 GTCGAC SapI 4074 GAAGAGC GCTCTTC(1/4) 3625 GCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 142 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BglI BglII BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI EcoO109I EcoRI EcoRII EcoRV EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI