##################################
# NO CUT ENZYMES (   62 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AgeI        
Alw44I      ApaLI       AscI        Asp718I     AvaI        
AviII       BamHI       BbrPI       BglII       BsaAI       
BsaBI       BsiWI       BsmBI       BsoBI       Bsp1407I    
BsrGI       BssHII      Bst1107I    BstBI       BstZ17I     
CpoI        EcoRI       EcoRV       EcoT22I     FseI        
FspI        HpaI        KpnI        MaeII       MamI        
MluI        NdeI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmlI        Psp1406I    PvuI        RsrII       SalI        
ScaI        SexAI       SfuI        SgrAI       SmaI        
SnaBI       SpeI        SspBI       SwaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AflII         103  CTTAAG               C^TTAAG
AflIII       2880  ACATGT               A^C[GA][CT]GT
Aor51HI      2216  AGCGCT               AGC^GCT
AseI         2756  ATTAAT               AT^TAAT
AsnI         2756  ATTAAT               AT^TAAT
Asp700I      4985  GAAAGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI         2228  TGGCCA               TGG^CCA
BbeI         2105  GGCGCC               GGCGC^C
BcgI         2389  CGAGAGCCTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        3116  GGATAC               GTATCC(6/5)
BfrI          103  CTTAAG               C^TTAAG
BsiEI        1698  CGGCCG               CG[GA][CT]^CG
BsmI         3795  GAATGC               GAATGC(1/-1)
BspDI        3877  ATCGAT               AT^CGAT
BspLU11I     2880  ACATGT               A^CATGT
BsrDI        2949  CATTGC               GCAATG(2/0)
ClaI         3877  ATCGAT               AT^CGAT
EagI         1698  CGGCCG               C^GGCCG
EclXI        1698  CGGCCG               C^GGCCG
Eco47III     2216  AGCGCT               AGC^GCT
Eco52I       1698  CGGCCG               C^GGCCG
KasI         2105  GGCGCC               G^GCGCC
MfeI          384  CAATTG               C^AATTG
MluNI        2228  TGGCCA               TGG^CCA
MscI         2228  TGGCCA               TGG^CCA
MunI          384  CAATTG               C^AATTG
NarI         2105  GGCGCC               GG^CGCC
NheI         2666  GCTAGC               G^CTAGC
PmeI         3311  GTTTAAAC             GTTT^AAAC
PshBI        2756  ATTAAT               AT^TAAT
SfoI         2105  GGCGCC               GGC^GCC
SphI         4829  GCATGC               GCATG^C
XbaI         3076  TCTAGA               T^CTAGA
XmnI         4985  GAAAGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccIII        897  TCCGGA               T^CCGGA
             1436  TCCGGA              
AcsI         3032  GAATTT               [GA]^AATT[CT]
             5311  AAATTT              
AhdI          639  GACCGTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2360  GACTCCCTGTC         
ApoI         3032  GAATTT               [GA]^AATT[CT]
             5311  AAATTT              
AspEI         639  GACCGTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2360  GACTCCCTGTC         
AvrII        3388  CCTAGG               C^CTAGG
             3553  CCTAGG              
BclI          490  TGATCA               T^GATCA
             3334  TGATCA              
BlnI         3388  CCTAGG               C^CTAGG
             3553  CCTAGG              
BsaWI         897  TCCGGA               [AT]^CCGG[AT]
             1436  TCCGGA              
BseAI         897  TCCGGA               T^CCGGA
             1436  TCCGGA              
BspEI         897  TCCGGA               T^CCGGA
             1436  TCCGGA              
BsrFI        2473  GCCGGC               [GA]^CCGG[CT]
             2650  GCCGGC              
Cfr10I       2473  GCCGGC               [GA]^CCGG[CT]
             2650  GCCGGC              
DraIII       4114  CACTTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4708  CACAAGGTG           
DrdI          242  GACCTCTGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3691  GACACAGCTGTC        
Eam1105I      639  GACCGTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2360  GACTCCCTGTC         
FbaI          490  TGATCA               T^GATCA
             3334  TGATCA              
HincII        636  GTTGAC               GT[CT]^[GA]AC
             1452  GTCAAC              
HindII        636  GTTGAC               GT[CT]^[GA]AC
             1452  GTCAAC              
HindIII      1473  AAGCTT               A^AGCTT
             4987  AAGCTT              
KspI         1622  CCGCGG               CCGC^GG
             1681  CCGCGG              
MroI          897  TCCGGA               T^CCGGA
             1436  TCCGGA              
NaeI         2473  GCCGGC               GCC^GGC
             2650  GCCGGC              
NgoMIV       2473  GCCGGC               G^CCGGC
             2650  GCCGGC              
PflMI         729  CCAGAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1087  CCAATGACTGG         
PshAI        3885  GACTAATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             4124  GACCACAGTC          
SacI         1990  GAGCTC               GAGCT^C
             4631  GAGCTC              
SacII        1622  CCGCGG               CCGC^GG
             1681  CCGCGG              
SfiI         2295  GGCCGAGAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             2304  GGCCGAGGTGGCC       
SspI         3082  AATATT               AAT^ATT
             3674  AATATT              
Van91I        729  CCAGAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1087  CCAATGACTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  131 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AocI        ApaI        AspHI       
AspI        AvaII       BanI        BanII       BbsI        
BbvCI       BbvI        BcnI        BfaI        BglI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspHI       BspMI       BsrBI       BsrI        
BssKI       BssSI       BstAPI      BstEII      BstF5I      
BstNI       BstPI       BstUI       BstXI       BstYI       
Bsu36I      Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraII       DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PflFI       PleI        PpuMI       
PstI        PvuII       RcaI        RsaI        SapI        
Sau3AI      Sau96I      SbfI        ScrFI       SfaNI       
SfcI        SmlI        Sse8387I    StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Tth111I     TthHB8I     XcmI        
XhoII