################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AatII Acc65I AccI AclI AgeI Alw44I ApaLI AscI Asp718I AvaI AviII BamHI BbrPI BglII BsaAI BsaBI BsiWI BsmBI BsoBI Bsp1407I BsrGI BssHII Bst1107I BstBI BstZ17I CpoI EcoRI EcoRV EcoT22I FseI FspI HpaI KpnI MaeII MamI MluI NdeI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmlI Psp1406I PvuI RsrII SalI ScaI SexAI SfuI SgrAI SmaI SnaBI SpeI SspBI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AflII 103 CTTAAG C^TTAAG AflIII 2880 ACATGT A^C[GA][CT]GT Aor51HI 2216 AGCGCT AGC^GCT AseI 2756 ATTAAT AT^TAAT AsnI 2756 ATTAAT AT^TAAT Asp700I 4985 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 2228 TGGCCA TGG^CCA BbeI 2105 GGCGCC GGCGC^C BcgI 2389 CGAGAGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3116 GGATAC GTATCC(6/5) BfrI 103 CTTAAG C^TTAAG BsiEI 1698 CGGCCG CG[GA][CT]^CG BsmI 3795 GAATGC GAATGC(1/-1) BspDI 3877 ATCGAT AT^CGAT BspLU11I 2880 ACATGT A^CATGT BsrDI 2949 CATTGC GCAATG(2/0) ClaI 3877 ATCGAT AT^CGAT EagI 1698 CGGCCG C^GGCCG EclXI 1698 CGGCCG C^GGCCG Eco47III 2216 AGCGCT AGC^GCT Eco52I 1698 CGGCCG C^GGCCG KasI 2105 GGCGCC G^GCGCC MfeI 384 CAATTG C^AATTG MluNI 2228 TGGCCA TGG^CCA MscI 2228 TGGCCA TGG^CCA MunI 384 CAATTG C^AATTG NarI 2105 GGCGCC GG^CGCC NheI 2666 GCTAGC G^CTAGC PmeI 3311 GTTTAAAC GTTT^AAAC PshBI 2756 ATTAAT AT^TAAT SfoI 2105 GGCGCC GGC^GCC SphI 4829 GCATGC GCATG^C XbaI 3076 TCTAGA T^CTAGA XmnI 4985 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccIII 897 TCCGGA T^CCGGA 1436 TCCGGA AcsI 3032 GAATTT [GA]^AATT[CT] 5311 AAATTT AhdI 639 GACCGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2360 GACTCCCTGTC ApoI 3032 GAATTT [GA]^AATT[CT] 5311 AAATTT AspEI 639 GACCGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2360 GACTCCCTGTC AvrII 3388 CCTAGG C^CTAGG 3553 CCTAGG BclI 490 TGATCA T^GATCA 3334 TGATCA BlnI 3388 CCTAGG C^CTAGG 3553 CCTAGG BsaWI 897 TCCGGA [AT]^CCGG[AT] 1436 TCCGGA BseAI 897 TCCGGA T^CCGGA 1436 TCCGGA BspEI 897 TCCGGA T^CCGGA 1436 TCCGGA BsrFI 2473 GCCGGC [GA]^CCGG[CT] 2650 GCCGGC Cfr10I 2473 GCCGGC [GA]^CCGG[CT] 2650 GCCGGC DraIII 4114 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG 4708 CACAAGGTG DrdI 242 GACCTCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3691 GACACAGCTGTC Eam1105I 639 GACCGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2360 GACTCCCTGTC FbaI 490 TGATCA T^GATCA 3334 TGATCA HincII 636 GTTGAC GT[CT]^[GA]AC 1452 GTCAAC HindII 636 GTTGAC GT[CT]^[GA]AC 1452 GTCAAC HindIII 1473 AAGCTT A^AGCTT 4987 AAGCTT KspI 1622 CCGCGG CCGC^GG 1681 CCGCGG MroI 897 TCCGGA T^CCGGA 1436 TCCGGA NaeI 2473 GCCGGC GCC^GGC 2650 GCCGGC NgoMIV 2473 GCCGGC G^CCGGC 2650 GCCGGC PflMI 729 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1087 CCAATGACTGG PshAI 3885 GACTAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4124 GACCACAGTC SacI 1990 GAGCTC GAGCT^C 4631 GAGCTC SacII 1622 CCGCGG CCGC^GG 1681 CCGCGG SfiI 2295 GGCCGAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 2304 GGCCGAGGTGGCC SspI 3082 AATATT AAT^ATT 3674 AATATT Van91I 729 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1087 CCAATGACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 131 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AocI ApaI AspHI AspI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BspMI BsrBI BsrI BssKI BssSI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PleI PpuMI PstI PvuII RcaI RsaI SapI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI SmlI Sse8387I StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XhoII