##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
Acc65I AflII AgeI AscI AseI
AsnI Asp718I AviII AvrII BfrI
BlnI BspDI BsrDI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI EcoNI
EcoT22I FseI FspI HpaI KpnI
KspI MfeI MluI MunI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
PvuI RsrII SacII SalI SbfI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 1343 GACGTC GACGT^C
AccIII 841 TCCGGA T^CCGGA
AflIII 2480 ACATGT A^C[GA][CT]GT
AhdI 2647 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 5341 AGCGCT AGC^GCT
AspEI 2647 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 4536 GCATGGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1425 GTATCC GTATCC(6/5)
BclI 577 TGATCA T^GATCA
BglII 4766 AGATCT A^GATCT
BsaBI 2126 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 841 TCCGGA T^CCGGA
BsiWI 4081 CGTACG C^GTACG
BsmI 3233 GAATGC GAATGC(1/-1)
BspEI 841 TCCGGA T^CCGGA
BspHI 511 TCATGA T^CATGA
BspLU11I 2480 ACATGT A^CATGT
BssSI 594 CACGAG CACGAG(-5/-1)
DraI 2230 TTTAAA TTT^AAA
DraIII 1699 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2759 CGGCCG C^GGCCG
Eam1105I 2647 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2759 CGGCCG C^GGCCG
Eco47III 5341 AGCGCT AGC^GCT
Eco52I 2759 CGGCCG C^GGCCG
EcoRI 3144 GAATTC G^AATTC
EcoRV 4412 GATATC GAT^ATC
FbaI 577 TGATCA T^GATCA
HincII 4924 GTTGAC GT[CT]^[GA]AC
HindII 4924 GTTGAC GT[CT]^[GA]AC
HindIII 2580 AAGCTT A^AGCTT
MamI 2126 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 841 TCCGGA T^CCGGA
NcoI 5307 CCATGG C^CATGG
NdeI 2609 CATATG CA^TATG
RcaI 511 TCATGA T^CATGA
ScaI 4377 AGTACT AGT^ACT
SexAI 2391 ACCAGGT A^CC[AT]GGT
SphI 917 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 3721 GTCTAC GT^[AC][GT]AC
4198 GTAGAC
AclI 1617 AACGTT AA^CGTT
3133 AACGTT
AcsI 2699 GAATTT [GA]^AATT[CT]
3144 GAATTC
Alw44I 2337 GTGCAC G^TGCAC
4239 GTGCAC
AlwNI 19 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
784 CAGTGACTG
AocI 3016 CCTAAGG CC^T[ACGT]AGG
4304 CCTGAGG
ApaLI 2337 GTGCAC G^TGCAC
4239 GTGCAC
ApoI 2699 GAATTT [GA]^AATT[CT]
3144 GAATTC
BamHI 3510 GGATCC G^GATCC
3788 GGATCC
BbeI 2049 GGCGCC GGCGC^C
2137 GGCGCC
Bsp1407I 607 TGTACA T^GTACA
3752 TGTACA
BsrGI 607 TGTACA T^GTACA
3752 TGTACA
BstAPI 1698 GCACTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5104 GCAGAGGATGC
Bsu36I 3016 CCTAAGG CC^T[ACGT]AGG
4304 CCTGAGG
DrdI 3661 GACGACCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5272 GACCAAGGAGTC
Eco81I 3016 CCTAAGG CC^T[ACGT]AGG
4304 CCTGAGG
KasI 2049 GGCGCC G^GCGCC
2137 GGCGCC
NarI 2049 GGCGCC GG^CGCC
2137 GGCGCC
NspI 917 GCATGC [GA]CATG^[CT]
2480 ACATGT
Psp1406I 1617 AACGTT AA^CGTT
3133 AACGTT
SfiI 398 GGCCGCCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1970 GGCCCGGGTGGCC
SfoI 2049 GGCGCC GGC^GCC
2137 GGCGCC
SmaI 1972 CCCGGG CCC^GGG
4641 CCCGGG
SspBI 607 TGTACA T^GTACA
3752 TGTACA
XmaCI 1972 CCCGGG C^CCGGG
4641 CCCGGG
XmaI 1972 CCCGGG C^CCGGG
4641 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI Asp700I AspHI AspI
AvaI AvaII BalI BanI BanII
BbrPI BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PflFI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmnI