################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AatII AccI AccIII AflII AflIII AgeI AscI AseI AsnI AvrII BfrI BglII BlnI BsaBI BseAI BsiWI BspDI BspEI BspLU11I BsrDI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI EcoO65I EcoRV EcoT22I FseI HindIII KspI MamI MfeI MluI MroI MunI NdeI NotI NruI NsiI NspV PacI PinAI PmeI PshAI PshBI PvuI RsrII SacII SalI ScaI SfiI SfuI SgrAI SnaBI SwaI XbaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 1421 GGTACC G^GTACC AclI 3189 AACGTT AA^CGTT AhdI 4788 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 2251 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1421 GGTACC G^GTACC AspEI 4788 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 275 TGCGCA TGC^GCA BbrPI 271 CACGTG CAC^GTG BcgI 3061 CGACGGCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1849 GGATAC GTATCC(6/5) BsaAI 271 CACGTG [CT]AC^GT[GA] BsmI 4996 GAATGC GAATGC(1/-1) Bsp1407I 4781 TGTACA T^GTACA BspHI 72 TCATGA T^CATGA BsrGI 4781 TGTACA T^GTACA BssHII 1225 GCGCGC G^CGCGC DrdI 76 GACCTATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 614 CGGCCG C^GGCCG Eam1105I 4788 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 614 CGGCCG C^GGCCG Eco52I 614 CGGCCG C^GGCCG EcoRI 5109 GAATTC G^AATTC FspI 275 TGCGCA TGC^GCA HpaI 4250 GTTAAC GTT^AAC KpnI 1421 GGTACC GGTAC^C NheI 4038 GCTAGC G^CTAGC PaeR7I 3513 CTCGAG C^TCGAG PmaCI 271 CACGTG CAC^GTG PmlI 271 CACGTG CAC^GTG Psp1406I 3189 AACGTT AA^CGTT RcaI 72 TCATGA T^CATGA SapI 2759 GCTCTTC GCTCTTC(1/4) SbfI 569 CCTGCAGG CCTGCA^GG SpeI 1480 ACTAGT A^CTAGT SphI 4192 GCATGC GCATG^C Sse8387I 569 CCTGCAGG CCTGCA^GG SspBI 4781 TGTACA T^GTACA SspI 4681 AATATT AAT^ATT XhoI 3513 CTCGAG C^TCGAG XmnI 2251 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### Alw44I 296 GTGCAC G^TGCAC 3155 GTGCAC Aor51HI 309 AGCGCT AGC^GCT 1306 AGCGCT ApaLI 296 GTGCAC G^TGCAC 3155 GTGCAC BclI 2241 TGATCA T^GATCA 5431 TGATCA BlpI 1309 GCTCAGC GC^T[ACGT]AGC 2684 GCTGAGC Bpu1102I 1309 GCTCAGC GC^T[ACGT]AGC 2684 GCTGAGC BssSI 1973 CACGAG CACGAG(-5/-1) 2381 CACGAG CelII 1309 GCTCAGC GC^T[ACGT]AGC 2684 GCTGAGC DraIII 299 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG 3617 CACCTGGTG Eco47III 309 AGCGCT AGC^GCT 1306 AGCGCT FbaI 2241 TGATCA T^GATCA 5431 TGATCA HincII 3783 GTTGAC GT[CT]^[GA]AC 4250 GTTAAC HindII 3783 GTTGAC GT[CT]^[GA]AC 4250 GTTAAC NaeI 1863 GCCGGC GCC^GGC 4553 GCCGGC NgoMIV 1863 GCCGGC G^CCGGC 4553 GCCGGC SexAI 771 ACCTGGT A^CC[AT]GGT 3618 ACCTGGT ######################################### # 3 OR MORE CUT ENZYMES ( 142 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AspI AvaI AvaII BalI BamHI BanI BanII BbeI BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmaCI XmaI