##################################
# NO CUT ENZYMES (   60 enzymes) #
##################################

AatII       AccI        AccIII      AflII       AflIII      
AgeI        AscI        AseI        AsnI        AvrII       
BfrI        BglII       BlnI        BsaBI       BseAI       
BsiWI       BspDI       BspEI       BspLU11I    BsrDI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
ClaI        CpoI        DraI        EcoO65I     EcoRV       
EcoT22I     FseI        HindIII     KspI        MamI        
MfeI        MluI        MroI        MunI        NdeI        
NotI        NruI        NsiI        NspV        PacI        
PinAI       PmeI        PshAI       PshBI       PvuI        
RsrII       SacII       SalI        ScaI        SfiI        
SfuI        SgrAI       SnaBI       SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

Acc65I       1421  GGTACC               G^GTACC
AclI         3189  AACGTT               AA^CGTT
AhdI         4788  GACAAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I      2251  GAAAGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1421  GGTACC               G^GTACC
AspEI        4788  GACAAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         275  TGCGCA               TGC^GCA
BbrPI         271  CACGTG               CAC^GTG
BcgI         3061  CGACGGCCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1849  GGATAC               GTATCC(6/5)
BsaAI         271  CACGTG               [CT]AC^GT[GA]
BsmI         4996  GAATGC               GAATGC(1/-1)
Bsp1407I     4781  TGTACA               T^GTACA
BspHI          72  TCATGA               T^CATGA
BsrGI        4781  TGTACA               T^GTACA
BssHII       1225  GCGCGC               G^CGCGC
DrdI           76  GACCTATGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          614  CGGCCG               C^GGCCG
Eam1105I     4788  GACAAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         614  CGGCCG               C^GGCCG
Eco52I        614  CGGCCG               C^GGCCG
EcoRI        5109  GAATTC               G^AATTC
FspI          275  TGCGCA               TGC^GCA
HpaI         4250  GTTAAC               GTT^AAC
KpnI         1421  GGTACC               GGTAC^C
NheI         4038  GCTAGC               G^CTAGC
PaeR7I       3513  CTCGAG               C^TCGAG
PmaCI         271  CACGTG               CAC^GTG
PmlI          271  CACGTG               CAC^GTG
Psp1406I     3189  AACGTT               AA^CGTT
RcaI           72  TCATGA               T^CATGA
SapI         2759  GCTCTTC              GCTCTTC(1/4)
SbfI          569  CCTGCAGG             CCTGCA^GG
SpeI         1480  ACTAGT               A^CTAGT
SphI         4192  GCATGC               GCATG^C
Sse8387I      569  CCTGCAGG             CCTGCA^GG
SspBI        4781  TGTACA               T^GTACA
SspI         4681  AATATT               AAT^ATT
XhoI         3513  CTCGAG               C^TCGAG
XmnI         2251  GAAAGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

Alw44I        296  GTGCAC               G^TGCAC
             3155  GTGCAC              
Aor51HI       309  AGCGCT               AGC^GCT
             1306  AGCGCT              
ApaLI         296  GTGCAC               G^TGCAC
             3155  GTGCAC              
BclI         2241  TGATCA               T^GATCA
             5431  TGATCA              
BlpI         1309  GCTCAGC              GC^T[ACGT]AGC
             2684  GCTGAGC             
Bpu1102I     1309  GCTCAGC              GC^T[ACGT]AGC
             2684  GCTGAGC             
BssSI        1973  CACGAG               CACGAG(-5/-1)
             2381  CACGAG              
CelII        1309  GCTCAGC              GC^T[ACGT]AGC
             2684  GCTGAGC             
DraIII        299  CACTTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3617  CACCTGGTG           
Eco47III      309  AGCGCT               AGC^GCT
             1306  AGCGCT              
FbaI         2241  TGATCA               T^GATCA
             5431  TGATCA              
HincII       3783  GTTGAC               GT[CT]^[GA]AC
             4250  GTTAAC              
HindII       3783  GTTGAC               GT[CT]^[GA]AC
             4250  GTTAAC              
NaeI         1863  GCCGGC               GCC^GGC
             4553  GCCGGC              
NgoMIV       1863  GCCGGC               G^CCGGC
             4553  GCCGGC              
SexAI         771  ACCTGGT              A^CC[AT]GGT
             3618  ACCTGGT             


#########################################
# 3 OR MORE CUT ENZYMES (  142 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       AocI        ApaI        
ApoI        AspHI       AspI        AvaI        AvaII       
BalI        BamHI       BanI        BanII       BbeI        
BbsI        BbvCI       BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BpuAI       BsaHI       
BsaI        BsaJI       BsaWI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstUI       BstXI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        Eco81I      EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
KasI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NarI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PflFI       PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfoI        SmaI        
SmlI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     Van91I      XcmI        XhoII       
XmaCI       XmaI