##################################
# NO CUT ENZYMES (   64 enzymes) #
##################################

AatII       AccIII      AcyI        AflII       Aor51HI     
ApaI        AscI        AspI        AvaI        AviII       
AvrII       BbeI        BcgI        BfrI        BglII       
BlnI        BsaBI       BsaHI       BseAI       BsiWI       
BsmBI       BsoBI       BspEI       BsrBI       BssHII      
Bst1107I    BstZ17I     CpoI        Eco47III    EcoNI       
FseI        FspI        Hin1I       HindIII     HpaI        
KasI        KspI        MamI        MluI        MroI        
NarI        NdeI        NheI        NotI        NruI        
PaeR7I      PflFI       PmeI        RsrII       SacII       
SalI        SapI        SbfI        SfiI        SfoI        
SgrAI       SmaI        SnaBI       Sse8387I    SwaI        
Tth111I     XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI         4620  GTCTAC               GT^[AC][GT]AC
AccII        4166  CGCG                 CG^CG
AclI          384  AACGTT               AA^CGTT
AflIII       1334  ACATGT               A^C[GA][CT]GT
AgeI         1578  ACCGGT               A^CCGGT
AhdI         3741  GACTGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI          536  ATTAAT               AT^TAAT
AsnI          536  ATTAAT               AT^TAAT
AspEI        3741  GACTGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI        3338  CACGTG               CAC^GTG
BglI         3985  GCCACATGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        3338  CACGTG               [CT]AC^GT[GA]
Bsp1407I     2103  TGTACA               T^GTACA
BspLU11I     1334  ACATGT               A^CATGT
BsrDI        3247  GCAATG               GCAATG(2/0)
BsrGI        2103  TGTACA               T^GTACA
BssSI        3880  CTCGTG               CACGAG(-5/-1)
BstAPI        320  GCATTTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         643  TTCGAA               TT^CGAA
BstUI        4166  CGCG                 CG^CG
BstXI        1263  CCAAAGAAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI         2938  GACTTATATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     3741  GACTGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          116  GAAGAG               CTCTTC(1/4)
HgaI         3851  GACGC                GACGC(5/10)
HincII       3323  GTCAAC               GT[CT]^[GA]AC
HindII       3323  GTCAAC               GT[CT]^[GA]AC
Ksp632I       116  GAAGAG               CTCTTC(1/4)
MvnI         4166  CGCG                 CG^CG
NaeI         1698  GCCGGC               GCC^GGC
NgoMIV       1698  GCCGGC               G^CCGGC
NspV          643  TTCGAA               TT^CGAA
PacI          533  TTAATTAA             TTAAT^TAA
PinAI        1578  ACCGGT               A^CCGGT
PmaCI        3338  CACGTG               CAC^GTG
PmlI         3338  CACGTG               CAC^GTG
PshBI         536  ATTAAT               AT^TAAT
Psp1406I      384  AACGTT               AA^CGTT
ScaI         3863  AGTACT               AGT^ACT
SexAI        1105  ACCAGGT              A^CC[AT]GGT
SfuI          643  TTCGAA               TT^CGAA
SpeI         4895  ACTAGT               A^CTAGT
SspBI        2103  TGTACA               T^GTACA
XbaI         5100  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AocI          732  CCTTAGG              CC^T[ACGT]AGG
             2335  CCTGAGG             
BamHI        1292  GGATCC               G^GATCC
             3566  GGATCC              
BbsI         2437  GAAGAC               GAAGAC(2/6)
             4712  GAAGAC              
BbvCI        2153  CCTCAGC              CCTCAGC(-5/-2)
             4544  CCTCAGC             
BclI         2251  TGATCA               T^GATCA
             2269  TGATCA              
BpuAI        2437  GAAGAC               GAAGAC(2/6)
             4712  GAAGAC              
BspDI        2778  ATCGAT               AT^CGAT
             3344  ATCGAT              
BspMI        1322  ACCTGC               ACCTGC(4/8)
             4235  ACCTGC              
BstEII       2387  GGTTACC              G^GT[ACGT]ACC
             2722  GGTCACC             
BstPI        2387  GGTTACC              G^GT[ACGT]ACC
             2722  GGTCACC             
BstYI        1292  GGATCC               [GA]^GATC[CT]
             3566  GGATCC              
Bsu36I        732  CCTTAGG              CC^T[ACGT]AGG
             2335  CCTGAGG             
ClaI         2778  ATCGAT               AT^CGAT
             3344  ATCGAT              
DraI         1723  TTTAAA               TTT^AAA
             4945  TTTAAA              
DraIII       3237  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4305  CACGCTGTG           
EagI           15  CGGCCG               C^GGCCG
             4325  CGGCCG              
EclXI          15  CGGCCG               C^GGCCG
             4325  CGGCCG              
Eco52I         15  CGGCCG               C^GGCCG
             4325  CGGCCG              
Eco81I        732  CCTTAGG              CC^T[ACGT]AGG
             2335  CCTGAGG             
EcoO65I      2387  GGTTACC              G^GT[ACGT]ACC
             2722  GGTCACC             
EcoRV         701  GATATC               GAT^ATC
             1676  GATATC              
EcoT22I       189  ATGCAT               ATGCA^T
             2926  ATGCAT              
FbaI         2251  TGATCA               T^GATCA
             2269  TGATCA              
MfeI          492  CAATTG               C^AATTG
             2139  CAATTG              
MflI         1292  GGATCC               [GA]^GATC[CT]
             3566  GGATCC              
MunI          492  CAATTG               C^AATTG
             2139  CAATTG              
NsiI          189  ATGCAT               ATGCA^T
             2926  ATGCAT              
PshAI        3464  GACTATCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             4152  GACCGGAGTC          
PstI          706  CTGCAG               CTGCA^G
             1594  CTGCAG              
SphI          187  GCATGC               GCATG^C
             2192  GCATGC              
StuI          736  AGGCCT               AGG^CCT
             3255  AGGCCT              
XhoII        1292  GGATCC               [GA]^GATC[CT]
             3566  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (  118 enzymes) #
#########################################

Acc65I      AciI        AcsI        AfaI        AluI        
Alw44I      AlwI        AlwNI       ApaLI       ApoI        
Asp700I     Asp718I     AspHI       AvaII       BalI        
BanI        BanII       BbvI        BciVI       BcnI        
BfaI        BlpI        BmyI        BpmI        Bpu1102I    
BsaI        BsaJI       BsaWI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    BspHI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EcoO109I    
EcoRI       EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
KpnI        MaeI        MaeII       MaeIII      MboI        
MboII       MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PvuI        
PvuII       RcaI        RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XmnI