################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AatII AccIII AcyI AflII Aor51HI ApaI AscI AspI AvaI AviII AvrII BbeI BcgI BfrI BglII BlnI BsaBI BsaHI BseAI BsiWI BsmBI BsoBI BspEI BsrBI BssHII Bst1107I BstZ17I CpoI Eco47III EcoNI FseI FspI Hin1I HindIII HpaI KasI KspI MamI MluI MroI NarI NdeI NheI NotI NruI PaeR7I PflFI PmeI RsrII SacII SalI SapI SbfI SfiI SfoI SgrAI SmaI SnaBI Sse8387I SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 4620 GTCTAC GT^[AC][GT]AC AccII 4166 CGCG CG^CG AclI 384 AACGTT AA^CGTT AflIII 1334 ACATGT A^C[GA][CT]GT AgeI 1578 ACCGGT A^CCGGT AhdI 3741 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 536 ATTAAT AT^TAAT AsnI 536 ATTAAT AT^TAAT AspEI 3741 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 3338 CACGTG CAC^GTG BglI 3985 GCCACATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 3338 CACGTG [CT]AC^GT[GA] Bsp1407I 2103 TGTACA T^GTACA BspLU11I 1334 ACATGT A^CATGT BsrDI 3247 GCAATG GCAATG(2/0) BsrGI 2103 TGTACA T^GTACA BssSI 3880 CTCGTG CACGAG(-5/-1) BstAPI 320 GCATTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 643 TTCGAA TT^CGAA BstUI 4166 CGCG CG^CG BstXI 1263 CCAAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 2938 GACTTATATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 3741 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 116 GAAGAG CTCTTC(1/4) HgaI 3851 GACGC GACGC(5/10) HincII 3323 GTCAAC GT[CT]^[GA]AC HindII 3323 GTCAAC GT[CT]^[GA]AC Ksp632I 116 GAAGAG CTCTTC(1/4) MvnI 4166 CGCG CG^CG NaeI 1698 GCCGGC GCC^GGC NgoMIV 1698 GCCGGC G^CCGGC NspV 643 TTCGAA TT^CGAA PacI 533 TTAATTAA TTAAT^TAA PinAI 1578 ACCGGT A^CCGGT PmaCI 3338 CACGTG CAC^GTG PmlI 3338 CACGTG CAC^GTG PshBI 536 ATTAAT AT^TAAT Psp1406I 384 AACGTT AA^CGTT ScaI 3863 AGTACT AGT^ACT SexAI 1105 ACCAGGT A^CC[AT]GGT SfuI 643 TTCGAA TT^CGAA SpeI 4895 ACTAGT A^CTAGT SspBI 2103 TGTACA T^GTACA XbaI 5100 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AocI 732 CCTTAGG CC^T[ACGT]AGG 2335 CCTGAGG BamHI 1292 GGATCC G^GATCC 3566 GGATCC BbsI 2437 GAAGAC GAAGAC(2/6) 4712 GAAGAC BbvCI 2153 CCTCAGC CCTCAGC(-5/-2) 4544 CCTCAGC BclI 2251 TGATCA T^GATCA 2269 TGATCA BpuAI 2437 GAAGAC GAAGAC(2/6) 4712 GAAGAC BspDI 2778 ATCGAT AT^CGAT 3344 ATCGAT BspMI 1322 ACCTGC ACCTGC(4/8) 4235 ACCTGC BstEII 2387 GGTTACC G^GT[ACGT]ACC 2722 GGTCACC BstPI 2387 GGTTACC G^GT[ACGT]ACC 2722 GGTCACC BstYI 1292 GGATCC [GA]^GATC[CT] 3566 GGATCC Bsu36I 732 CCTTAGG CC^T[ACGT]AGG 2335 CCTGAGG ClaI 2778 ATCGAT AT^CGAT 3344 ATCGAT DraI 1723 TTTAAA TTT^AAA 4945 TTTAAA DraIII 3237 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG 4305 CACGCTGTG EagI 15 CGGCCG C^GGCCG 4325 CGGCCG EclXI 15 CGGCCG C^GGCCG 4325 CGGCCG Eco52I 15 CGGCCG C^GGCCG 4325 CGGCCG Eco81I 732 CCTTAGG CC^T[ACGT]AGG 2335 CCTGAGG EcoO65I 2387 GGTTACC G^GT[ACGT]ACC 2722 GGTCACC EcoRV 701 GATATC GAT^ATC 1676 GATATC EcoT22I 189 ATGCAT ATGCA^T 2926 ATGCAT FbaI 2251 TGATCA T^GATCA 2269 TGATCA MfeI 492 CAATTG C^AATTG 2139 CAATTG MflI 1292 GGATCC [GA]^GATC[CT] 3566 GGATCC MunI 492 CAATTG C^AATTG 2139 CAATTG NsiI 189 ATGCAT ATGCA^T 2926 ATGCAT PshAI 3464 GACTATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4152 GACCGGAGTC PstI 706 CTGCAG CTGCA^G 1594 CTGCAG SphI 187 GCATGC GCATG^C 2192 GCATGC StuI 736 AGGCCT AGG^CCT 3255 AGGCCT XhoII 1292 GGATCC [GA]^GATC[CT] 3566 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### Acc65I AciI AcsI AfaI AluI Alw44I AlwI AlwNI ApaLI ApoI Asp700I Asp718I AspHI AvaII BalI BanI BanII BbvI BciVI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BsrFI BsrI BssKI BstF5I BstNI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI KpnI MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PvuI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XmnI