##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AgeI AocI ApaI AscI
Asp718I AspI AviII BbrPI BbvCI
BsgI BsiEI BsiWI BsmBI BspDI
BspLU11I BsrFI BssHII BstBI Bsu36I
Cfr10I ClaI CpoI EagI EclXI
Eco52I Eco81I EcoRI FseI FspI
KpnI MfeI MluI MunI NaeI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PvuI RsrII SacI
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SpeI SphI
Sse8387I StuI SwaI Tth111I XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 1921 TCCGGA T^CCGGA
AflII 2849 CTTAAG C^TTAAG
AflIII 3017 ACGTGT A^C[GA][CT]GT
AhdI 2319 GACAAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 5844 GTGCAC G^TGCAC
Aor51HI 1975 AGCGCT AGC^GCT
ApaLI 5844 GTGCAC G^TGCAC
AspEI 2319 GACAAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 361 CTCGAG C^[CT]CG[GA]G
AvrII 1739 CCTAGG C^CTAGG
BbeI 1867 GGCGCC GGCGC^C
BcgI 3128 GCAGCTTCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2690 GGATAC GTATCC(6/5)
BclI 5810 TGATCA T^GATCA
BfrI 2849 CTTAAG C^TTAAG
BglI 652 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1739 CCTAGG C^CTAGG
BsaAI 1989 TACGTA [CT]AC^GT[GA]
BsaBI 5416 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1921 TCCGGA T^CCGGA
BsmI 5125 GCATTC GAATGC(1/-1)
BsoBI 361 CTCGAG C^[CT]CG[GA]G
BspEI 1921 TCCGGA T^CCGGA
BspHI 3827 TCATGA T^CATGA
BssSI 2092 CACGAG CACGAG(-5/-1)
Bst1107I 3949 GTATAC GTA^TAC
BstEII 3543 GGTCACC G^GT[ACGT]ACC
BstPI 3543 GGTCACC G^GT[ACGT]ACC
BstZ17I 3949 GTATAC GTA^TAC
DrdI 3055 GACGTGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2319 GACAAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1975 AGCGCT AGC^GCT
EcoNI 5727 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3543 GGTCACC G^GT[ACGT]ACC
EcoRV 1296 GATATC GAT^ATC
FbaI 5810 TGATCA T^GATCA
HgaI 2289 GACGC GACGC(5/10)
KasI 1867 GGCGCC G^GCGCC
MamI 5416 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1921 TCCGGA T^CCGGA
NarI 1867 GGCGCC GG^CGCC
NdeI 4672 CATATG CA^TATG
PaeR7I 361 CTCGAG C^TCGAG
PstI 5849 CTGCAG CTGCA^G
RcaI 3827 TCATGA T^CATGA
SfoI 1867 GGCGCC GGC^GCC
SnaBI 1989 TACGTA TAC^GTA
XhoI 361 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AclI 1597 AACGTT AA^CGTT
4011 AACGTT
Asp700I 45 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3460 GAAGCAATTC
BanI 1086 GGTGCC G^G[CT][GA]CC
1867 GGCGCC
BcnI 341 CCGGG CC^[GC]GG
2081 CCCGG
BsaWI 1921 TCCGGA [AT]^CCGG[AT]
3175 ACCGGA
Bsp1407I 529 TGTACA T^GTACA
3560 TGTACA
BspMI 4128 ACCTGC ACCTGC(4/8)
220 GCAGGT
BsrGI 529 TGTACA T^GTACA
3560 TGTACA
BstAPI 1269 GCAATGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1335 GCAGATCGTGC
DraIII 963 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
4546 CACAAAGTG
EcoT22I 386 ATGCAT ATGCA^T
5482 ATGCAT
HincII 1776 GTTAAC GT[CT]^[GA]AC
4728 GTTAAC
HindII 1776 GTTAAC GT[CT]^[GA]AC
4728 GTTAAC
HindIII 1653 AAGCTT A^AGCTT
5303 AAGCTT
HpaI 1776 GTTAAC GTT^AAC
4728 GTTAAC
NciI 341 CCGGG CC^[GC]GG
2081 CCCGG
NcoI 1477 CCATGG C^CATGG
3366 CCATGG
NsiI 386 ATGCAT ATGCA^T
5482 ATGCAT
PflMI 825 CCACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2418 CCAAGCAGTGG
Psp1406I 1597 AACGTT AA^CGTT
4011 AACGTT
PvuII 2022 CAGCTG CAG^CTG
3027 CAGCTG
SapI 1905 GAAGAGC GCTCTTC(1/4)
5000 GAAGAGC
SspBI 529 TGTACA T^GTACA
3560 TGTACA
SspI 1119 AATATT AAT^ATT
3882 AATATT
Van91I 825 CCACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2418 CCAAGCAGTGG
XmnI 45 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3460 GAAGCAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AatII AccI AccII AciI AcsI
AcyI AfaI AluI AlwI AlwNI
ApoI AseI AsnI AspHI AvaII
BalI BamHI BanII BbsI BbvI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrBI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV NspI
PleI PpuMI PshBI RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII