##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AgeI Aor51HI AscI
AseI AsnI Asp700I Asp718I AviII
AvrII BbeI BbrPI BcgI BciVI
BclI BglII BlnI BsaAI BsaBI
BsiEI BsiWI Bsp1407I BspDI BsrGI
BssHII Bst1107I BstXI BstZ17I ClaI
CpoI DraIII EagI EclXI Eco47III
Eco52I EcoRV EcoT22I FbaI FseI
FspI HpaI KasI KpnI MamI
MfeI MluI MunI NarI NotI
NruI NsiI PacI PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RsrII SalI ScaI SfiI SfoI
SnaBI SpeI SspBI SwaI XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 2021 TCCGGA T^CCGGA
AclI 5002 AACGTT AA^CGTT
AcyI 236 GACGCC G[GA]^CG[CT]C
AflII 2038 CTTAAG C^TTAAG
AhdI 2361 GACTCGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 965 GTGCAC G^TGCAC
ApaLI 965 GTGCAC G^TGCAC
AspEI 2361 GACTCGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 3218 TGGCCA TGG^CCA
BamHI 558 GGATCC G^GATCC
BanI 126 GGCACC G^G[CT][GA]CC
BfrI 2038 CTTAAG C^TTAAG
BsaHI 236 GACGCC G[GA]^CG[CT]C
BsaWI 2021 TCCGGA [AT]^CCGG[AT]
BseAI 2021 TCCGGA T^CCGGA
BsmBI 2281 GAGACG CGTCTC(1/5)
BspEI 2021 TCCGGA T^CCGGA
BspHI 4517 TCATGA T^CATGA
BspMI 4904 GCAGGT ACCTGC(4/8)
BsrDI 3681 CATTGC GCAATG(2/0)
BssSI 3730 CTCGTG CACGAG(-5/-1)
BstBI 858 TTCGAA TT^CGAA
BstEII 3822 GGTGACC G^GT[ACGT]ACC
BstPI 3822 GGTGACC G^GT[ACGT]ACC
DraI 5039 TTTAAA TTT^AAA
DrdI 160 GACCATGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2361 GACTCGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3822 GGTGACC G^GT[ACGT]ACC
Hin1I 236 GACGCC G[GA]^CG[CT]C
KspI 955 CCGCGG CCGC^GG
MluNI 3218 TGGCCA TGG^CCA
MroI 2021 TCCGGA T^CCGGA
MscI 3218 TGGCCA TGG^CCA
NaeI 2324 GCCGGC GCC^GGC
NcoI 162 CCATGG C^CATGG
NdeI 1054 CATATG CA^TATG
NgoMIV 2324 GCCGGC G^CCGGC
NheI 591 GCTAGC G^CTAGC
NspV 858 TTCGAA TT^CGAA
Psp1406I 5002 AACGTT AA^CGTT
RcaI 4517 TCATGA T^CATGA
SacII 955 CCGCGG CCGC^GG
SapI 4360 GCTCTTC GCTCTTC(1/4)
SbfI 2880 CCTGCAGG CCTGCA^GG
SexAI 3107 ACCAGGT A^CC[AT]GGT
SfuI 858 TTCGAA TT^CGAA
SgrAI 2323 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 2880 CCTGCAGG CCTGCA^GG
SspI 5048 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AfaI 263 GTAC GT^AC
1680 GTAC
AspI 928 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3658 GACAGTGTC
BsmI 2460 GAATGC GAATGC(1/-1)
3811 GCATTC
BsrFI 2324 GCCGGC [GA]^CCGG[CT]
4920 GCCGGT
Cfr10I 2324 GCCGGC [GA]^CCGG[CT]
4920 GCCGGT
EcoRI 2317 GAATTC G^AATTC
3652 GAATTC
HincII 677 GTCAAC GT[CT]^[GA]AC
1605 GTTGAC
HindII 677 GTCAAC GT[CT]^[GA]AC
1605 GTTGAC
HindIII 3593 AAGCTT A^AGCTT
4065 AAGCTT
PflFI 928 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3658 GACAGTGTC
RsaI 263 GTAC GT^AC
1680 GTAC
SphI 581 GCATGC GCATG^C
1712 GCATGC
StuI 530 AGGCCT AGG^CCT
3764 AGGCCT
Tth111I 928 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3658 GACAGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AccII AciI AcsI AflIII
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspLU11I BsrBI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PaeR7I PflMI PleI PpuMI PstI
PvuII SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoI XhoII XmaCI XmaI