################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII AccII AccIII AcyI AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AspEI AvaI AviII BamHI BanII BbeI BbvCI BcgI BcnI BglI BlpI Bpu1102I BsaHI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BsrBI BsrFI BssHII BssSI BstEII BstPI BstUI Bsu36I CelII CfoI Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I FauI FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KspI MluI MroI MvnI NaeI NarI NciI NdeI NgoMIV NotI NruI PacI PaeR7I PinAI PmeI PshAI PvuI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SgrAI SmaI SnaBI Sse8387I SwaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 398 GGTACC G^GTACC AciI 1132 CCGC CCGC(-3/-1) AflII 4265 CTTAAG C^TTAAG Alw44I 4950 GTGCAC G^TGCAC ApaLI 4950 GTGCAC G^TGCAC Asp718I 398 GGTACC G^GTACC AspI 2349 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 5271 CCTAGG C^CTAGG BbrPI 705 CACGTG CAC^GTG BbsI 2226 GTCTTC GAAGAC(2/6) BclI 1540 TGATCA T^GATCA BfrI 4265 CTTAAG C^TTAAG BlnI 5271 CCTAGG C^CTAGG BpuAI 2226 GTCTTC GAAGAC(2/6) BsaBI 515 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2034 TCCGGT [AT]^CCGG[AT] Bst1107I 2302 GTATAC GTA^TAC BstBI 474 TTCGAA TT^CGAA BstXI 29 CCAAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 2302 GTATAC GTA^TAC DraII 3798 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoNI 5190 CCTCAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 3798 AGGTCCT [GA]G^G[ACGT]CC[CT] FbaI 1540 TGATCA T^GATCA HapII 2035 CCGG C^CGG HgaI 2939 GACGC GACGC(5/10) HpaII 2035 CCGG C^CGG KpnI 398 GGTACC GGTAC^C MamI 515 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 792 CAGCTG C[AC]G^C[GT]G MspI 2035 CCGG C^CGG NspV 474 TTCGAA TT^CGAA PflFI 2349 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 705 CACGTG CAC^GTG PmlI 705 CACGTG CAC^GTG PpuMI 3798 AGGTCCT [GA]G^G[AT]CC[CT] PstI 3214 CTGCAG CTGCA^G PvuII 792 CAGCTG CAG^CTG SexAI 3105 ACCTGGT A^CC[AT]GGT SfuI 474 TTCGAA TT^CGAA SpeI 3259 ACTAGT A^CTAGT SphI 4257 GCATGC GCATG^C StuI 2237 AGGCCT AGG^CCT Tth111I 2349 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### BanI 236 GGTGCC G^G[CT][GA]CC 398 GGTACC BpmI 3170 CTCCAG CTGGAG(16/14) 4758 CTCCAG BsaI 1128 GAGACC GGTCTC(1/5) 3167 GGTCTC BspHI 1232 TCATGA T^CATGA 4403 TCATGA BspMI 233 GCAGGT ACCTGC(4/8) 1625 GCAGGT BstAPI 428 GCATCTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1012 GCAAAAGTTGC DraIII 1199 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG 3104 CACCTGGTG DsaI 35 CCATGG C^C[GA][CT]GG 3074 CCATGG EcoRI 1752 GAATTC G^AATTC 4365 GAATTC HincII 1116 GTCAAC GT[CT]^[GA]AC 5143 GTCAAC HindII 1116 GTCAAC GT[CT]^[GA]AC 5143 GTCAAC HindIII 242 AAGCTT A^AGCTT 2607 AAGCTT MfeI 4344 CAATTG C^AATTG 5092 CAATTG MunI 4344 CAATTG C^AATTG 5092 CAATTG NcoI 35 CCATGG C^CATGG 3074 CCATGG NheI 2666 GCTAGC G^CTAGC 4149 GCTAGC PflMI 2345 CCAAGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3069 CCATGCCATGG RcaI 1232 TCATGA T^CATGA 4403 TCATGA ScaI 3337 AGTACT AGT^ACT 3542 AGTACT SmlI 949 CTCAAG C^T[CT][GA]AG 4265 CTTAAG SspI 959 AATATT AAT^ATT 5222 AATATT TaqI 475 TCGA T^CGA 871 TCGA TthHB8I 475 TCGA T^CGA 871 TCGA Van91I 2345 CCAAGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3069 CCATGCCATGG XbaI 1069 TCTAGA T^CTAGA 1814 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AccI AclI AcsI AfaI AflIII AluI AlwI ApoI AseI AsnI Asp700I AspHI AvaII BalI BbvI BciVI BfaI BglII BmyI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspLU11I BsrDI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI EaeI EarI EcoRII EcoRV EcoT14I EcoT22I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NsiI NspI PleI PshBI Psp1406I RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII XmnI