##################################
# NO CUT ENZYMES (   95 enzymes) #
##################################

AatII       AccII       AccIII      AcyI        AgeI        
AhdI        AlwNI       AocI        Aor51HI     ApaI        
AscI        AspEI       AvaI        AviII       BamHI       
BanII       BbeI        BbvCI       BcgI        BcnI        
BglI        BlpI        Bpu1102I    BsaHI       BseAI       
BsiEI       BsiWI       BsmBI       BsoBI       BspDI       
BspEI       BsrBI       BsrFI       BssHII      BssSI       
BstEII      BstPI       BstUI       Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     FauI        FseI        FspI        
HaeII       HhaI        Hin1I       HinP1I      HpaI        
KasI        KspI        MluI        MroI        MvnI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NotI        NruI        PacI        PaeR7I      PinAI       
PmeI        PshAI       PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SfiI        
SfoI        SgrAI       SmaI        SnaBI       Sse8387I    
SwaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I        398  GGTACC               G^GTACC
AciI         1132  CCGC                 CCGC(-3/-1)
AflII        4265  CTTAAG               C^TTAAG
Alw44I       4950  GTGCAC               G^TGCAC
ApaLI        4950  GTGCAC               G^TGCAC
Asp718I       398  GGTACC               G^GTACC
AspI         2349  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        5271  CCTAGG               C^CTAGG
BbrPI         705  CACGTG               CAC^GTG
BbsI         2226  GTCTTC               GAAGAC(2/6)
BclI         1540  TGATCA               T^GATCA
BfrI         4265  CTTAAG               C^TTAAG
BlnI         5271  CCTAGG               C^CTAGG
BpuAI        2226  GTCTTC               GAAGAC(2/6)
BsaBI         515  GATCCGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        2034  TCCGGT               [AT]^CCGG[AT]
Bst1107I     2302  GTATAC               GTA^TAC
BstBI         474  TTCGAA               TT^CGAA
BstXI          29  CCAAGACCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      2302  GTATAC               GTA^TAC
DraII        3798  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoNI        5190  CCTCAAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     3798  AGGTCCT              [GA]G^G[ACGT]CC[CT]
FbaI         1540  TGATCA               T^GATCA
HapII        2035  CCGG                 C^CGG
HgaI         2939  GACGC                GACGC(5/10)
HpaII        2035  CCGG                 C^CGG
KpnI          398  GGTACC               GGTAC^C
MamI          515  GATCCGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I        792  CAGCTG               C[AC]G^C[GT]G
MspI         2035  CCGG                 C^CGG
NspV          474  TTCGAA               TT^CGAA
PflFI        2349  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         705  CACGTG               CAC^GTG
PmlI          705  CACGTG               CAC^GTG
PpuMI        3798  AGGTCCT              [GA]G^G[AT]CC[CT]
PstI         3214  CTGCAG               CTGCA^G
PvuII         792  CAGCTG               CAG^CTG
SexAI        3105  ACCTGGT              A^CC[AT]GGT
SfuI          474  TTCGAA               TT^CGAA
SpeI         3259  ACTAGT               A^CTAGT
SphI         4257  GCATGC               GCATG^C
StuI         2237  AGGCCT               AGG^CCT
Tth111I      2349  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

BanI          236  GGTGCC               G^G[CT][GA]CC
              398  GGTACC              
BpmI         3170  CTCCAG               CTGGAG(16/14)
             4758  CTCCAG              
BsaI         1128  GAGACC               GGTCTC(1/5)
             3167  GGTCTC              
BspHI        1232  TCATGA               T^CATGA
             4403  TCATGA              
BspMI         233  GCAGGT               ACCTGC(4/8)
             1625  GCAGGT              
BstAPI        428  GCATCTATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1012  GCAAAAGTTGC         
DraIII       1199  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3104  CACCTGGTG           
DsaI           35  CCATGG               C^C[GA][CT]GG
             3074  CCATGG              
EcoRI        1752  GAATTC               G^AATTC
             4365  GAATTC              
HincII       1116  GTCAAC               GT[CT]^[GA]AC
             5143  GTCAAC              
HindII       1116  GTCAAC               GT[CT]^[GA]AC
             5143  GTCAAC              
HindIII       242  AAGCTT               A^AGCTT
             2607  AAGCTT              
MfeI         4344  CAATTG               C^AATTG
             5092  CAATTG              
MunI         4344  CAATTG               C^AATTG
             5092  CAATTG              
NcoI           35  CCATGG               C^CATGG
             3074  CCATGG              
NheI         2666  GCTAGC               G^CTAGC
             4149  GCTAGC              
PflMI        2345  CCAAGACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3069  CCATGCCATGG         
RcaI         1232  TCATGA               T^CATGA
             4403  TCATGA              
ScaI         3337  AGTACT               AGT^ACT
             3542  AGTACT              
SmlI          949  CTCAAG               C^T[CT][GA]AG
             4265  CTTAAG              
SspI          959  AATATT               AAT^ATT
             5222  AATATT              
TaqI          475  TCGA                 T^CGA
              871  TCGA                
TthHB8I       475  TCGA                 T^CGA
              871  TCGA                
Van91I       2345  CCAAGACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3069  CCATGCCATGG         
XbaI         1069  TCTAGA               T^CTAGA
             1814  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   94 enzymes) #
#########################################

AccI        AclI        AcsI        AfaI        AflIII      
AluI        AlwI        ApoI        AseI        AsnI        
Asp700I     AspHI       AvaII       BalI        BbvI        
BciVI       BfaI        BglII       BmyI        BsaAI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
Bsp1407I    BspLU11I    BsrDI       BsrGI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraI        EaeI        
EarI        EcoRII      EcoRV       EcoT14I     EcoT22I     
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       NsiI        NspI        
PleI        PshBI       Psp1406I    RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SspBI       
StyI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       XhoII       XmnI