##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI AscI Asp718I AspEI
AviII AvrII BbrPI BcgI BlnI
BsaAI BsaWI BseAI BsiWI BsmBI
BspDI BspEI BspHI Bst1107I BstZ17I
ClaI CpoI Eam1105I EcoRI EcoRV
FseI FspI KpnI MfeI MluI
MroI MunI NdeI NheI NotI
NruI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I RcaI
RsrII SalI SfiI SgrAI SnaBI
SpeI SphI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcsI 4598 AAATTC [GA]^AATT[CT]
AflII 5346 CTTAAG C^TTAAG
AocI 2753 CCTGAGG CC^T[ACGT]AGG
ApoI 4598 AAATTC [GA]^AATT[CT]
BalI 5198 TGGCCA TGG^CCA
BbsI 1706 GAAGAC GAAGAC(2/6)
BciVI 3925 GTATCC GTATCC(6/5)
BclI 2109 TGATCA T^GATCA
BfrI 5346 CTTAAG C^TTAAG
BpuAI 1706 GAAGAC GAAGAC(2/6)
BsaBI 4069 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 1941 TGTACA T^GTACA
BsrBI 618 GAGCGG CCGCTC(-3/-3)
BsrGI 1941 TGTACA T^GTACA
BssSI 1439 CTCGTG CACGAG(-5/-1)
BstAPI 2897 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4601 TTCGAA TT^CGAA
BstEII 1681 GGTCACC G^GT[ACGT]ACC
BstPI 1681 GGTCACC G^GT[ACGT]ACC
Bsu36I 2753 CCTGAGG CC^T[ACGT]AGG
DrdI 4832 GACAGAATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2546 CGGCCG C^GGCCG
EclXI 2546 CGGCCG C^GGCCG
Eco52I 2546 CGGCCG C^GGCCG
Eco81I 2753 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1681 GGTCACC G^GT[ACGT]ACC
FbaI 2109 TGATCA T^GATCA
HindIII 4448 AAGCTT A^AGCTT
HpaI 4577 GTTAAC GTT^AAC
MamI 4069 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 5198 TGGCCA TGG^CCA
MscI 5198 TGGCCA TGG^CCA
NspV 4601 TTCGAA TT^CGAA
PvuI 1318 CGATCG CGAT^CG
SacI 2301 GAGCTC GAGCT^C
SapI 3043 GCTCTTC GCTCTTC(1/4)
ScaI 5407 AGTACT AGT^ACT
SexAI 2670 ACCAGGT A^CC[AT]GGT
SfuI 4601 TTCGAA TT^CGAA
SspBI 1941 TGTACA T^GTACA
SspI 4 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
Asp700I 1729 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4034 GAAGATCTTC
BsiEI 1318 CGATCG CG[GA][CT]^CG
2546 CGGCCG
BssHII 324 GCGCGC G^CGCGC
2063 GCGCGC
EcoT22I 1136 ATGCAT ATGCA^T
5388 ATGCAT
KspI 354 CCGCGG CCGC^GG
3929 CCGCGG
NcoI 2020 CCATGG C^CATGG
4089 CCATGG
NsiI 1136 ATGCAT ATGCA^T
5388 ATGCAT
PpuMI 2077 GGGACCT [GA]G^G[AT]CC[CT]
2757 AGGACCC
PvuII 2328 CAGCTG CAG^CTG
3546 CAGCTG
SacII 354 CCGCGG CCGC^GG
3929 CCGCGG
TaqI 1081 TCGA T^CGA
4602 TCGA
TthHB8I 1081 TCGA T^CGA
4602 TCGA
XmnI 1729 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4034 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI Aor51HI
ApaI ApaLI AseI AsnI AspHI
AspI AvaI AvaII BamHI BanI
BanII BbeI BbvCI BbvI BcnI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspLU11I BspMI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI Eco47III
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflFI
PflMI PleI PshBI PstI RsaI
Sau3AI Sau96I SbfI ScrFI SfaNI
SfcI SfoI SmaI SmlI Sse8387I
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I Van91I
XcmI XhoII XmaCI XmaI