##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AclI AflII AflIII AhdI
Aor51HI AscI AseI AsnI AspEI
BbrPI BclI BfrI BglII BsaBI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI DrdI
Eam1105I Eco47III EcoO65I EcoRV EcoT22I
FbaI FseI HindIII KspI MamI
MfeI MluI MunI NaeI NdeI
NgoMIV NotI NruI NsiI NspV
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI SacII SalI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SphI SspBI SwaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccI 821 GTCTAC GT^[AC][GT]AC
AccIII 2693 TCCGGA T^CCGGA
AgeI 2262 ACCGGT A^CCGGT
Alw44I 672 GTGCAC G^TGCAC
ApaLI 672 GTGCAC G^TGCAC
Asp700I 3173 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1047 TGCGCA TGC^GCA
BamHI 4614 GGATCC G^GATCC
BcgI 3108 CGATACTTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 446 TACGTG [CT]AC^GT[GA]
BseAI 2693 TCCGGA T^CCGGA
BsiEI 706 CGGCCG CG[GA][CT]^CG
BsiWI 685 CGTACG C^GTACG
BspEI 2693 TCCGGA T^CCGGA
DraIII 4086 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 706 CGGCCG C^GGCCG
EclXI 706 CGGCCG C^GGCCG
Eco52I 706 CGGCCG C^GGCCG
EcoNI 4372 CCTCACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3589 GAATTC G^AATTC
FspI 1047 TGCGCA TGC^GCA
HpaI 4160 GTTAAC GTT^AAC
MroI 2693 TCCGGA T^CCGGA
NheI 1265 GCTAGC G^CTAGC
PacI 5032 TTAATTAA TTAAT^TAA
PinAI 2262 ACCGGT A^CCGGT
PshAI 1786 GACGCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 281 GAAGAGC GCTCTTC(1/4)
SpeI 4444 ACTAGT A^CTAGT
SspI 4482 AATATT AAT^ATT
XbaI 4803 TCTAGA T^CTAGA
XmnI 3173 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 44 CGCG CG^CG
475 CGCG
AcsI 3589 GAATTC [GA]^AATT[CT]
4937 AAATTT
ApoI 3589 GAATTC [GA]^AATT[CT]
4937 AAATTT
BsaI 2653 GAGACC GGTCTC(1/5)
4865 GAGACC
BsaWI 2262 ACCGGT [AT]^CCGG[AT]
2693 TCCGGA
BsmBI 81 CGTCTC CGTCTC(1/5)
1120 CGTCTC
BsmI 1356 GCATTC GAATGC(1/-1)
3413 GCATTC
BsrFI 846 GCCGGT [GA]^CCGG[CT]
2262 ACCGGT
BstUI 44 CGCG CG^CG
475 CGCG
Cfr10I 846 GCCGGT [GA]^CCGG[CT]
2262 ACCGGT
CpoI 2483 CGGTCCG CG^G[AT]CCG
2544 CGGACCG
DraI 4934 TTTAAA TTT^AAA
5022 TTTAAA
EarI 281 GAAGAG CTCTTC(1/4)
4807 GAAGAG
HincII 4160 GTTAAC GT[CT]^[GA]AC
4351 GTTGAC
HindII 4160 GTTAAC GT[CT]^[GA]AC
4351 GTTGAC
Ksp632I 281 GAAGAG CTCTTC(1/4)
4807 GAAGAG
MvnI 44 CGCG CG^CG
475 CGCG
NcoI 364 CCATGG C^CATGG
2033 CCATGG
PpuMI 2024 AGGACCT [GA]G^G[AT]CC[CT]
3022 GGGACCC
RsrII 2483 CGGTCCG CG^G[AT]CCG
2544 CGGACCG
SbfI 748 CCTGCAGG CCTGCA^GG
2089 CCTGCAGG
Sse8387I 748 CCTGCAGG CCTGCA^GG
2089 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
Acc65I AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI Asp718I
AspHI AspI AvaI AvaII AvrII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BciVI BcnI BfaI
BglI BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI KpnI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PaeR7I PflFI
PflMI PleI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoI XhoII XmaCI
XmaI