##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI Asp718I AspEI AviII
BamHI BbeI BglI BsaHI BsaWI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BsrBI BsrFI BsrGI BssHII
BstBI Bsu36I Cfr10I ClaI CpoI
EagI Eam1105I EclXI Eco52I Eco81I
EcoRV EcoT22I FseI FspI Hin1I
KasI KpnI KspI MluI MroI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PaeR7I PinAI
PmeI Psp1406I PvuI RsrII SacII
SalI ScaI SexAI SfiI SfoI
SfuI SgrAI SspBI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 5236 CGCG CG^CG
Aor51HI 2000 AGCGCT AGC^GCT
AseI 4474 ATTAAT AT^TAAT
AsnI 4474 ATTAAT AT^TAAT
AvaI 5443 CCCGGG C^[CT]CG[GA]G
AvrII 2930 CCTAGG C^CTAGG
BbrPI 2992 CACGTG CAC^GTG
BcgI 4710 CGAGGGCGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 842 TGATCA T^GATCA
BlnI 2930 CCTAGG C^CTAGG
BlpI 787 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 787 GCTGAGC GC^T[ACGT]AGC
BsaBI 3370 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 4627 CGTCTC CGTCTC(1/5)
BsoBI 5443 CCCGGG C^[CT]CG[GA]G
BstAPI 1518 GCAACTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2888 GGTGACC G^GT[ACGT]ACC
BstPI 2888 GGTGACC G^GT[ACGT]ACC
BstUI 5236 CGCG CG^CG
CelII 787 GCTGAGC GC^T[ACGT]AGC
DraIII 4266 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4127 GACTCCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2000 AGCGCT AGC^GCT
EcoO65I 2888 GGTGACC G^GT[ACGT]ACC
EcoRI 651 GAATTC G^AATTC
FauI 168 GCGGG CCCGC(4/6)
FbaI 842 TGATCA T^GATCA
HincII 4492 GTTAAC GT[CT]^[GA]AC
HindII 4492 GTTAAC GT[CT]^[GA]AC
HpaI 4492 GTTAAC GTT^AAC
MamI 3370 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 5236 CGCG CG^CG
PmaCI 2992 CACGTG CAC^GTG
PmlI 2992 CACGTG CAC^GTG
PpuMI 3322 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 4474 ATTAAT AT^TAAT
PvuII 2732 CAGCTG CAG^CTG
SapI 862 GCTCTTC GCTCTTC(1/4)
SbfI 656 CCTGCAGG CCTGCA^GG
SmaI 5443 CCCGGG CCC^GGG
SnaBI 2275 TACGTA TAC^GTA
SpeI 3831 ACTAGT A^CTAGT
SphI 3577 GCATGC GCATG^C
Sse8387I 656 CCTGCAGG CCTGCA^GG
SwaI 4508 ATTTAAAT ATTT^AAAT
XmaCI 5443 CCCGGG C^CCGGG
XmaI 5443 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Asp700I 859 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2290 GAAATCATTC
BanI 2665 GGTGCC G^G[CT][GA]CC
5033 GGCACC
BglII 1496 AGATCT A^GATCT
5052 AGATCT
BsaAI 2275 TACGTA [CT]AC^GT[GA]
2992 CACGTG
BseRI 2110 GAGGAG GAGGAG(10/8)
5077 GAGGAG
Bst1107I 401 GTATAC GTA^TAC
496 GTATAC
BstXI 67 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2720 CCATCAGCCTGG
BstZ17I 401 GTATAC GTA^TAC
496 GTATAC
DraII 2023 GGGGCCT [GA]G^G[ACGT]CC[CT]
3322 GGGTCCT
DsaI 608 CCATGG C^C[GA][CT]GG
3012 CCATGG
EcoNI 3100 CCTCCACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4825 CCTAAGTAAGG
EcoO109I 2023 GGGGCCT [GA]G^G[ACGT]CC[CT]
3322 GGGTCCT
HgaI 713 GACGC GACGC(5/10)
1855 GCGTC
MfeI 1641 CAATTG C^AATTG
2697 CAATTG
MspA1I 2732 CAGCTG C[AC]G^C[GT]G
5479 CCGCTG
MunI 1641 CAATTG C^AATTG
2697 CAATTG
NcoI 608 CCATGG C^CATGG
3012 CCATGG
PacI 727 TTAATTAA TTAAT^TAA
3616 TTAATTAA
PshAI 272 GACATAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1091 GACCTAAGTC
SacI 2755 GAGCTC GAGCT^C
3309 GAGCTC
StuI 3132 AGGCCT AGG^CCT
5083 AGGCCT
XcmI 2781 CCAGCCAATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4687 CCAAAGCAAGACTGG
XmnI 859 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2290 GAAATCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApoI
AspHI AspI AvaII BalI BanII
BbsI BbvCI BbvI BciVI BcnI
BfaI BfrI BmyI BpmI BpuAI
BsaI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspHI BspLU11I BspMI BsrDI BsrI
BssKI BssSI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI EaeI EarI EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspI
MvaI MwoI NciI NdeI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XbaI XhoII