##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BbeI BclI BlnI BlpI Bpu1102I
BsaHI BsaWI BseAI BsgI BsiWI
BspDI BspEI BsrFI BssHII BssSI
Bst1107I BstBI BstZ17I CelII Cfr10I
ClaI CpoI DraIII DrdI Eam1105I
EcoRV FbaI FseI FspI Hin1I
KasI KpnI KspI MluI MroI
NaeI NarI NgoMIV NotI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI Sse8387I Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccI 3829 GTCTAC GT^[AC][GT]AC
Alw44I 5561 GTGCAC G^TGCAC
Aor51HI 156 AGCGCT AGC^GCT
ApaI 807 GGGCCC GGGCC^C
ApaLI 5561 GTGCAC G^TGCAC
BbvCI 2528 GCTGAGG CCTCAGC(-5/-2)
BcgI 5583 CGAAGCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1220 GGATAC GTATCC(6/5)
BglI 1659 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI 1018 CGGCCG CG[GA][CT]^CG
BsmI 754 GCATTC GAATGC(1/-1)
BspHI 81 TCATGA T^CATGA
BspLU11I 3823 ACATGT A^CATGT
BspMI 3196 GCAGGT ACCTGC(4/8)
BstAPI 5426 GCATGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI 1018 CGGCCG C^GGCCG
EclXI 1018 CGGCCG C^GGCCG
Eco47III 156 AGCGCT AGC^GCT
Eco52I 1018 CGGCCG C^GGCCG
EcoT22I 752 ATGCAT ATGCA^T
HaeII 156 AGCGCT [GA]GCGC^[CT]
MfeI 5776 CAATTG C^AATTG
MunI 5776 CAATTG C^AATTG
NheI 3972 GCTAGC G^CTAGC
NruI 3875 TCGCGA TCG^CGA
NsiI 752 ATGCAT ATGCA^T
PaeR7I 648 CTCGAG C^TCGAG
PshAI 5774 GACAATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 81 TCATGA T^CATGA
SmaI 1071 CCCGGG CCC^GGG
SpeI 3383 ACTAGT A^CTAGT
SphI 173 GCATGC GCATG^C
XhoI 648 CTCGAG C^TCGAG
XmaCI 1071 CCCGGG C^CCGGG
XmaI 1071 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 381 CGCG CG^CG
3876 CGCG
AflII 4739 CTTAAG C^TTAAG
5100 CTTAAG
AocI 4410 CCTCAGG CC^T[ACGT]AGG
4589 CCTCAGG
Asp700I 2941 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3690 GAATCCTTTC
BbrPI 1427 CACGTG CAC^GTG
4447 CACGTG
BfrI 4739 CTTAAG C^TTAAG
5100 CTTAAG
BglII 1282 AGATCT A^GATCT
1303 AGATCT
BsaI 1036 GAGACC GGTCTC(1/5)
5316 GGTCTC
BsmBI 1453 CGTCTC CGTCTC(1/5)
1101 GAGACG
BsrBI 970 CCGCTC CCGCTC(-3/-3)
4812 GAGCGG
BstEII 4720 GGTCACC G^GT[ACGT]ACC
5439 GGTTACC
BstPI 4720 GGTCACC G^GT[ACGT]ACC
5439 GGTTACC
BstUI 381 CGCG CG^CG
3876 CGCG
Bsu36I 4410 CCTCAGG CC^T[ACGT]AGG
4589 CCTCAGG
CfoI 157 GCGC GCG^C
5328 GCGC
Eco81I 4410 CCTCAGG CC^T[ACGT]AGG
4589 CCTCAGG
EcoNI 1607 CCTCGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1619 CCTGCCCAAGG
EcoO65I 4720 GGTCACC G^GT[ACGT]ACC
5439 GGTTACC
HhaI 157 GCGC GCG^C
5328 GCGC
HinP1I 157 GCGC G^CGC
5328 GCGC
HindIII 4653 AAGCTT A^AGCTT
4918 AAGCTT
HpaI 1013 GTTAAC GTT^AAC
4153 GTTAAC
MvnI 381 CGCG CG^CG
3876 CGCG
NdeI 2374 CATATG CA^TATG
2845 CATATG
PmaCI 1427 CACGTG CAC^GTG
4447 CACGTG
PmlI 1427 CACGTG CAC^GTG
4447 CACGTG
SacI 3857 GAGCTC GAGCT^C
4693 GAGCTC
ScaI 1199 AGTACT AGT^ACT
1908 AGTACT
SspI 2992 AATATT AAT^ATT
4609 AATATT
StuI 4414 AGGCCT AGG^CCT
5386 AGGCCT
XmnI 2941 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3690 GAATCCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaBI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StyI
SwaI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII