##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AccIII AclI AflII AhdI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspEI AviII BalI BciVI
BclI BfrI BseAI BsiWI BspDI
BspEI BspHI BspLU11I BssHII Bst1107I
BstEII BstPI BstZ17I ClaI Eam1105I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HpaI KpnI MluI
MluNI MroI MscI NaeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PmeI PshBI Psp1406I PvuI
RcaI ScaI SexAI SfiI SgrAI
SnaBI SpeI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AgeI 1783 ACCGGT A^CCGGT
Aor51HI 2033 AGCGCT AGC^GCT
Asp700I 9 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4305 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3022 CCTAGG C^CTAGG
BbrPI 385 CACGTG CAC^GTG
BcgI 2118 GCATCTGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 5110 AGATCT A^GATCT
BlnI 3022 CCTAGG C^CTAGG
BsaBI 654 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 120 CGGCCG CG[GA][CT]^CG
BsmI 3374 GCATTC GAATGC(1/-1)
Bsp1407I 1508 TGTACA T^GTACA
BsrGI 1508 TGTACA T^GTACA
BstBI 3560 TTCGAA TT^CGAA
CpoI 1123 CGGACCG CG^G[AT]CCG
DraIII 2019 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 120 CGGCCG C^GGCCG
EclXI 120 CGGCCG C^GGCCG
Eco47III 2033 AGCGCT AGC^GCT
Eco52I 120 CGGCCG C^GGCCG
MamI 654 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4995 CAATTG C^AATTG
MunI 4995 CAATTG C^AATTG
NdeI 2279 CATATG CA^TATG
NspV 3560 TTCGAA TT^CGAA
PflFI 4305 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1783 ACCGGT A^CCGGT
PmaCI 385 CACGTG CAC^GTG
PmlI 385 CACGTG CAC^GTG
RsrII 1123 CGGACCG CG^G[AT]CCG
SalI 3836 GTCGAC G^TCGAC
SfuI 3560 TTCGAA TT^CGAA
SspBI 1508 TGTACA T^GTACA
Tth111I 4305 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3147 TCTAGA T^CTAGA
XmnI 9 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AatII 1930 GACGTC GACGT^C
5379 GACGTC
AccI 328 GTCTAC GT^[AC][GT]AC
3836 GTCGAC
AflIII 2303 ACACGT A^C[GA][CT]GT
3641 ACGTGT
ApaI 3625 GGGCCC GGGCC^C
4791 GGGCCC
BamHI 480 GGATCC G^GATCC
566 GGATCC
BglI 2435 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4616 GCCAGAGAGGC
BlpI 867 GCTGAGC GC^T[ACGT]AGC
3362 GCTGAGC
Bpu1102I 867 GCTGAGC GC^T[ACGT]AGC
3362 GCTGAGC
BsaAI 385 CACGTG [CT]AC^GT[GA]
3640 TACGTG
BsaWI 727 ACCGGA [AT]^CCGG[AT]
1783 ACCGGT
BsrDI 380 CATTGC GCAATG(2/0)
1962 CATTGC
CelII 867 GCTGAGC GC^T[ACGT]AGC
3362 GCTGAGC
HindIII 11 AAGCTT A^AGCTT
5054 AAGCTT
MslI 3192 CACCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
5524 CATTCTTGTG
PflMI 3774 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4029 CCAGGGCCTGG
SbfI 1176 CCTGCAGG CCTGCA^GG
1488 CCTGCAGG
SmlI 3512 CTCAAG C^T[CT][GA]AG
5262 CTCAAG
Sse8387I 1176 CCTGCAGG CCTGCA^GG
1488 CCTGCAGG
TaqI 3561 TCGA T^CGA
3837 TCGA
TthHB8I 3561 TCGA T^CGA
3837 TCGA
Van91I 3774 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4029 CCAGGGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DrdI DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PshAI PstI
PvuII RsaI SacI SacII SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SphI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XhoII XmaCI XmaI