##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AgeI        Aor51HI     ApaI        AscI        
Asp718I     AvrII       BalI        BbeI        BbrPI       
BcgI        BlnI        BsaHI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BspEI       BsrBI       
BssHII      BssSI       Bst1107I    BstUI       BstZ17I     
CpoI        DraIII      EagI        EclXI       Eco47III    
Eco52I      FseI        HgaI        Hin1I       HindIII     
KasI        KpnI        KspI        MfeI        MluI        
MluNI       MroI        MscI        MunI        MvnI        
NarI        NdeI        NheI        NotI        NruI        
PacI        PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SnaBI       Sse8387I    
StuI        SwaI        Van91I      XcmI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI         3926  GTCTAC               GT^[AC][GT]AC
AflII        3000  CTTAAG               C^TTAAG
AhdI         5252  GACCTGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI        3331  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I      5361  GAAACTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        5252  GACCTGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         3620  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         4754  CTCGAG               C^[CT]CG[GA]G
AviII        3848  TGCGCA               TGC^GCA
BamHI        1683  GGATCC               G^GATCC
BciVI        3647  GGATAC               GTATCC(6/5)
BfrI         3000  CTTAAG               C^TTAAG
BglI          411  GCCTTCGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI         1665  CTCCAG               CTGGAG(16/14)
BsoBI        4754  CTCGAG               C^[CT]CG[GA]G
BspHI        1104  TCATGA               T^CATGA
BsrFI        3990  GCCGGC               [GA]^CCGG[CT]
BstAPI       4148  GCAGATAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        2902  TTCGAA               TT^CGAA
BstEII       3490  GGTTACC              G^GT[ACGT]ACC
BstPI        3490  GGTTACC              G^GT[ACGT]ACC
Cfr10I       3990  GCCGGC               [GA]^CCGG[CT]
DrdI         3632  GACCTTTATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         2930  CCATGG               C^C[GA][CT]GG
EaeI          242  TGGCCG               [CT]^GGCC[GA]
Eam1105I     5252  GACCTGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        4982  CCTCAGATAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      3490  GGTTACC              G^GT[ACGT]ACC
EcoRI        2732  GAATTC               G^AATTC
EcoRV        4538  GATATC               GAT^ATC
FspI         3848  TGCGCA               TGC^GCA
HaeII        1543  AGCGCC               [GA]GCGC^[CT]
NaeI         3990  GCCGGC               GCC^GGC
NcoI         2930  CCATGG               C^CATGG
NgoMIV       3990  GCCGGC               G^CCGGC
NspV         2902  TTCGAA               TT^CGAA
PaeR7I       4754  CTCGAG               C^TCGAG
PflFI        3620  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        4978  AGGTCCT              [GA]G^G[AT]CC[CT]
RcaI         1104  TCATGA               T^CATGA
SapI         3868  GCTCTTC              GCTCTTC(1/4)
ScaI         2511  AGTACT               AGT^ACT
SfuI         2902  TTCGAA               TT^CGAA
SpeI         3178  ACTAGT               A^CTAGT
SphI          362  GCATGC               GCATG^C
Tth111I      3620  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         4754  CTCGAG               C^TCGAG
XmnI         5361  GAAACTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

Alw44I       4110  GTGCAC               G^TGCAC
             5723  GTGCAC              
AocI         1397  CCTCAGG              CC^T[ACGT]AGG
             2432  CCTCAGG             
ApaLI        4110  GTGCAC               G^TGCAC
             5723  GTGCAC              
AseI         2113  ATTAAT               AT^TAAT
             4304  ATTAAT              
AsnI         2113  ATTAAT               AT^TAAT
             4304  ATTAAT              
BanI         1448  GGTGCC               G^G[CT][GA]CC
             2363  GGCACC              
BglII         674  AGATCT               A^GATCT
             2754  AGATCT              
BlpI         3078  GCTTAGC              GC^T[ACGT]AGC
             4657  GCTAAGC             
Bpu1102I     3078  GCTTAGC              GC^T[ACGT]AGC
             4657  GCTAAGC             
BsaAI        1750  CACGTA               [CT]AC^GT[GA]
             2372  CACGTA              
BspDI         441  ATCGAT               AT^CGAT
             1016  ATCGAT              
BspLU11I     4329  ACATGT               A^CATGT
             5056  ACATGT              
Bsu36I       1397  CCTCAGG              CC^T[ACGT]AGG
             2432  CCTCAGG             
CelII        3078  GCTTAGC              GC^T[ACGT]AGC
             4657  GCTAAGC             
ClaI          441  ATCGAT               AT^CGAT
             1016  ATCGAT              
Eco81I       1397  CCTCAGG              CC^T[ACGT]AGG
             2432  CCTCAGG             
EcoT22I       140  ATGCAT               ATGCA^T
              775  ATGCAT              
HpaI         1022  GTTAAC               GTT^AAC
             5443  GTTAAC              
NsiI          140  ATGCAT               ATGCA^T
              775  ATGCAT              
PshBI        2113  ATTAAT               AT^TAAT
             4304  ATTAAT              
PstI         4995  CTGCAG               CTGCA^G
             5265  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (  108 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        ApoI        AspHI       AvaII       BanII       
BbsI        BbvCI       BbvI        BclI        BcnI        
BfaI        BmyI        BpuAI       BsaBI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
Bsp1407I    BspMI       BsrDI       BsrGI       BsrI        
BssKI       BstF5I      BstNI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       EarI        EcoO109I    EcoRII      
EcoT14I     FauI        FbaI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MamI        
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspBI       SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XbaI        XhoII