##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

AatII       AccI        AclI        AgeI        Alw44I      
Aor51HI     ApaLI       AscI        AviII       AvrII       
BalI        BamHI       BclI        BglI        BlnI        
BsaBI       BsiEI       BsiWI       BsmBI       BspDI       
BspLU11I    BspMI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     ClaI        CpoI        
EaeI        EagI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     FbaI        FseI        FspI        
HgaI        HpaI        KspI        MamI        MluI        
MluNI       MscI        NdeI        NheI        NotI        
NruI        NspI        NspV        PaeR7I      PinAI       
PmeI        PshAI       Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        SfiI        SfuI        
SgrAI       SnaBI       SphI        Sse8387I    SwaI        
XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

Acc65I       2603  GGTACC               G^GTACC
AccIII       4920  TCCGGA               T^CCGGA
AcyI           86  GGCGCC               G[GA]^CG[CT]C
AhdI         4790  GACCTTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          458  CCTCAGG              CC^T[ACGT]AGG
AseI         4526  ATTAAT               AT^TAAT
AsnI         4526  ATTAAT               AT^TAAT
Asp700I      2760  GAAAATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2603  GGTACC               G^GTACC
AspEI        4790  GACCTTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1023  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI           86  GGCGCC               GGCGC^C
BbrPI         447  CACGTG               CAC^GTG
BciVI         906  GGATAC               GTATCC(6/5)
BsaAI         447  CACGTG               [CT]AC^GT[GA]
BsaHI          86  GGCGCC               G[GA]^CG[CT]C
BseAI        4920  TCCGGA               T^CCGGA
BspEI        4920  TCCGGA               T^CCGGA
BsrDI        2598  GCAATG               GCAATG(2/0)
BstXI        3451  CCAGAGGTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        458  CCTCAGG              CC^T[ACGT]AGG
Eam1105I     4790  GACCTTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        458  CCTCAGG              CC^T[ACGT]AGG
EcoRV        3705  GATATC               GAT^ATC
Hin1I          86  GGCGCC               G[GA]^CG[CT]C
KasI           86  GGCGCC               G^GCGCC
KpnI         2603  GGTACC               GGTAC^C
MfeI         2310  CAATTG               C^AATTG
MroI         4920  TCCGGA               T^CCGGA
MunI         2310  CAATTG               C^AATTG
NaeI          150  GCCGGC               GCC^GGC
NarI           86  GGCGCC               GG^CGCC
NcoI         4257  CCATGG               C^CATGG
NgoMIV        150  GCCGGC               G^CCGGC
PacI          864  TTAATTAA             TTAAT^TAA
PflFI        1023  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         292  CCAATGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         447  CACGTG               CAC^GTG
PmlI          447  CACGTG               CAC^GTG
PshBI        4526  ATTAAT               AT^TAAT
SexAI        1454  ACCTGGT              A^CC[AT]GGT
SfoI           86  GGCGCC               GGC^GCC
SpeI         3668  ACTAGT               A^CTAGT
Tth111I      1023  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        292  CCAATGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         2760  GAAAATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccII          20  CGCG                 CG^CG
               67  CGCG                
AflII        2514  CTTAAG               C^TTAAG
             4732  CTTAAG              
AflIII        446  ACACGT               A^C[GA][CT]GT
             2320  ACGTGT              
BanI           86  GGCGCC               G^G[CT][GA]CC
             2603  GGTACC              
BcgI         3526  CGAGTGATGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             4440  GCAGAGGTCTCG        
BfrI         2514  CTTAAG               C^TTAAG
             4732  CTTAAG              
BlpI          103  GCTGAGC              GC^T[ACGT]AGC
             2223  GCTGAGC             
Bpu1102I      103  GCTGAGC              GC^T[ACGT]AGC
             2223  GCTGAGC             
BsaI         2735  GGTCTC               GGTCTC(1/5)
             4445  GGTCTC              
BsaWI         507  ACCGGA               [AT]^CCGG[AT]
             4920  TCCGGA              
Bsp1407I      731  TGTACA               T^GTACA
             2206  TGTACA              
BsrBI          98  GAGCGG               CCGCTC(-3/-3)
              106  GAGCGG              
BsrFI         150  GCCGGC               [GA]^CCGG[CT]
              316  ACCGGC              
BsrGI         731  TGTACA               T^GTACA
             2206  TGTACA              
BssSI        1821  CACGAG               CACGAG(-5/-1)
               34  CTCGTG              
BstUI          20  CGCG                 CG^CG
               67  CGCG                
CelII         103  GCTGAGC              GC^T[ACGT]AGC
             2223  GCTGAGC             
Cfr10I        150  GCCGGC               [GA]^CCGG[CT]
              316  ACCGGC              
DraIII        475  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3523  CACCGAGTG           
DrdI         1530  GACAATGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2315  GACAAACGTGTC        
DsaI          331  CCGTGG               C^C[GA][CT]GG
             4257  CCATGG              
EcoRI         949  GAATTC               G^AATTC
             1543  GAATTC              
HindIII      1400  AAGCTT               A^AGCTT
             4714  AAGCTT              
MaeII         448  ACGT                 A^CGT
             2320  ACGT                
MvnI           20  CGCG                 CG^CG
               67  CGCG                
SapI          257  GAAGAGC              GCTCTTC(1/4)
             2869  GCTCTTC             
SmaI           28  CCCGGG               CCC^GGG
               90  CCCGGG              
SspBI         731  TGTACA               T^GTACA
             2206  TGTACA              
StuI         2941  AGGCCT               AGG^CCT
             3540  AGGCCT              
XmaCI          28  CCCGGG               C^CCGGG
               90  CCCGGG              
XmaI           28  CCCGGG               C^CCGGG
               90  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  109 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApaI        ApoI        AspHI       AvaI        
AvaII       BanII       BbsI        BbvCI       BbvI        
BcnI        BfaI        BglII       BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspHI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DraII       EarI        EcoO109I    EcoRII      EcoT14I     
EcoT22I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NsiI        PleI        PpuMI       
PstI        PvuII       RcaI        RsaI        SacI        
Sau3AI      Sau96I      ScaI        ScrFI       SfaNI       
SfcI        SmlI        SspI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XbaI        XhoII