################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII AccI AclI AgeI Alw44I Aor51HI ApaLI AscI AviII AvrII BalI BamHI BclI BglI BlnI BsaBI BsiEI BsiWI BsmBI BspDI BspLU11I BspMI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI EaeI EagI EclXI Eco47III Eco52I EcoNI EcoO65I FbaI FseI FspI HgaI HpaI KspI MamI MluI MluNI MscI NdeI NheI NotI NruI NspI NspV PaeR7I PinAI PmeI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfuI SgrAI SnaBI SphI Sse8387I SwaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 2603 GGTACC G^GTACC AccIII 4920 TCCGGA T^CCGGA AcyI 86 GGCGCC G[GA]^CG[CT]C AhdI 4790 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 458 CCTCAGG CC^T[ACGT]AGG AseI 4526 ATTAAT AT^TAAT AsnI 4526 ATTAAT AT^TAAT Asp700I 2760 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2603 GGTACC G^GTACC AspEI 4790 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1023 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 86 GGCGCC GGCGC^C BbrPI 447 CACGTG CAC^GTG BciVI 906 GGATAC GTATCC(6/5) BsaAI 447 CACGTG [CT]AC^GT[GA] BsaHI 86 GGCGCC G[GA]^CG[CT]C BseAI 4920 TCCGGA T^CCGGA BspEI 4920 TCCGGA T^CCGGA BsrDI 2598 GCAATG GCAATG(2/0) BstXI 3451 CCAGAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 458 CCTCAGG CC^T[ACGT]AGG Eam1105I 4790 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 458 CCTCAGG CC^T[ACGT]AGG EcoRV 3705 GATATC GAT^ATC Hin1I 86 GGCGCC G[GA]^CG[CT]C KasI 86 GGCGCC G^GCGCC KpnI 2603 GGTACC GGTAC^C MfeI 2310 CAATTG C^AATTG MroI 4920 TCCGGA T^CCGGA MunI 2310 CAATTG C^AATTG NaeI 150 GCCGGC GCC^GGC NarI 86 GGCGCC GG^CGCC NcoI 4257 CCATGG C^CATGG NgoMIV 150 GCCGGC G^CCGGC PacI 864 TTAATTAA TTAAT^TAA PflFI 1023 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 292 CCAATGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 447 CACGTG CAC^GTG PmlI 447 CACGTG CAC^GTG PshBI 4526 ATTAAT AT^TAAT SexAI 1454 ACCTGGT A^CC[AT]GGT SfoI 86 GGCGCC GGC^GCC SpeI 3668 ACTAGT A^CTAGT Tth111I 1023 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 292 CCAATGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 2760 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccII 20 CGCG CG^CG 67 CGCG AflII 2514 CTTAAG C^TTAAG 4732 CTTAAG AflIII 446 ACACGT A^C[GA][CT]GT 2320 ACGTGT BanI 86 GGCGCC G^G[CT][GA]CC 2603 GGTACC BcgI 3526 CGAGTGATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4440 GCAGAGGTCTCG BfrI 2514 CTTAAG C^TTAAG 4732 CTTAAG BlpI 103 GCTGAGC GC^T[ACGT]AGC 2223 GCTGAGC Bpu1102I 103 GCTGAGC GC^T[ACGT]AGC 2223 GCTGAGC BsaI 2735 GGTCTC GGTCTC(1/5) 4445 GGTCTC BsaWI 507 ACCGGA [AT]^CCGG[AT] 4920 TCCGGA Bsp1407I 731 TGTACA T^GTACA 2206 TGTACA BsrBI 98 GAGCGG CCGCTC(-3/-3) 106 GAGCGG BsrFI 150 GCCGGC [GA]^CCGG[CT] 316 ACCGGC BsrGI 731 TGTACA T^GTACA 2206 TGTACA BssSI 1821 CACGAG CACGAG(-5/-1) 34 CTCGTG BstUI 20 CGCG CG^CG 67 CGCG CelII 103 GCTGAGC GC^T[ACGT]AGC 2223 GCTGAGC Cfr10I 150 GCCGGC [GA]^CCGG[CT] 316 ACCGGC DraIII 475 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG 3523 CACCGAGTG DrdI 1530 GACAATGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2315 GACAAACGTGTC DsaI 331 CCGTGG C^C[GA][CT]GG 4257 CCATGG EcoRI 949 GAATTC G^AATTC 1543 GAATTC HindIII 1400 AAGCTT A^AGCTT 4714 AAGCTT MaeII 448 ACGT A^CGT 2320 ACGT MvnI 20 CGCG CG^CG 67 CGCG SapI 257 GAAGAGC GCTCTTC(1/4) 2869 GCTCTTC SmaI 28 CCCGGG CCC^GGG 90 CCCGGG SspBI 731 TGTACA T^GTACA 2206 TGTACA StuI 2941 AGGCCT AGG^CCT 3540 AGGCCT XmaCI 28 CCCGGG C^CCGGG 90 CCCGGG XmaI 28 CCCGGG C^CCGGG 90 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII BanII BbsI BbvCI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NsiI PleI PpuMI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII