##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AocI        ApoI        
AscI        AseI        AsnI        Asp718I     AspI        
BciVI       BclI        BfrI        BglII       BsaWI       
BseAI       BsiWI       BsmBI       BsmI        BspDI       
BspEI       BspHI       Bst1107I    BstBI       BstZ17I     
Bsu36I      ClaI        DraI        Eco81I      EcoRI       
FbaI        FseI        HindIII     HpaI        KpnI        
MfeI        MroI        MunI        NdeI        NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshBI       Psp1406I    
PvuI        RcaI        SalI        ScaI        SfuI        
SgrAI       SnaBI       SpeI        StuI        SwaI        
Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AhdI         4772  GACAAGTAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI       954  AGCGCT               AGC^GCT
Asp700I       870  GAAGGCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        4772  GACAAGTAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        2482  CCTAGG               C^CTAGG
BamHI         860  GGATCC               G^GATCC
BlnI         2482  CCTAGG               C^CTAGG
BsaBI        2514  GATTCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         2200  GAGACC               GGTCTC(1/5)
BsiEI          26  CGGCCG               CG[GA][CT]^CG
Bsp1407I     4100  TGTACA               T^GTACA
BsrGI        4100  TGTACA               T^GTACA
BssSI        1927  CTCGTG               CACGAG(-5/-1)
BstEII       1011  GGTCACC              G^GT[ACGT]ACC
BstPI        1011  GGTCACC              G^GT[ACGT]ACC
CpoI         4304  CGGACCG              CG^G[AT]CCG
DrdI          700  GACAACCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI           26  CGGCCG               C^GGCCG
Eam1105I     4772  GACAAGTAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI          26  CGGCCG               C^GGCCG
Eco47III      954  AGCGCT               AGC^GCT
Eco52I         26  CGGCCG               C^GGCCG
EcoNI         392  CCTCCTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1011  GGTCACC              G^GT[ACGT]ACC
EcoRV        1446  GATATC               GAT^ATC
HincII       3898  GTCAAC               GT[CT]^[GA]AC
HindII       3898  GTCAAC               GT[CT]^[GA]AC
MamI         2514  GATTCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI          368  ACGCGT               A^CGCGT
MseI         5121  TTAA                 T^TAA
RsrII        4304  CGGACCG              CG^G[AT]CCG
SacI          289  GAGCTC               GAGCT^C
SapI         1683  GCTCTTC              GCTCTTC(1/4)
SexAI         704  ACCAGGT              A^CC[AT]GGT
SspBI        4100  TGTACA               T^GTACA
SspI         5117  AATATT               AAT^ATT
Tru9I        5121  TTAA                 T^TAA
XmnI          870  GAAGGCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AviII         186  TGCGCA               TGC^GCA
             4346  TGCGCA              
BbrPI        1272  CACGTG               CAC^GTG
             4054  CACGTG              
BbvCI        2253  GCTGAGG              CCTCAGC(-5/-2)
             2850  GCTGAGG             
BspLU11I     2810  ACATGT               A^CATGT
             4586  ACATGT              
BssHII        349  GCGCGC               G^CGCGC
              456  GCGCGC              
EcoT22I      4332  ATGCAT               ATGCA^T
             4811  ATGCAT              
FspI          186  TGCGCA               TGC^GCA
             4346  TGCGCA              
KspI          135  CCGCGG               CCGC^GG
              879  CCGCGG              
NsiI         4332  ATGCAT               ATGCA^T
             4811  ATGCAT              
PmaCI        1272  CACGTG               CAC^GTG
             4054  CACGTG              
PmlI         1272  CACGTG               CAC^GTG
             4054  CACGTG              
PshAI        2315  GACTGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3004  GACTGCAGTC          
SacII         135  CCGCGG               CCGC^GG
              879  CCGCGG              
SbfI         1387  CCTGCAGG             CCTGCA^GG
             3969  CCTGCAGG            
SfiI          140  GGCCAGCGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             2227  GGCCTCAGTGGCC       
SmlI          670  CTCAAG               C^T[CT][GA]AG
             2506  CTCAAG              
SphI         2666  GCATGC               GCATG^C
             2783  GCATGC              
Sse8387I     1387  CCTGCAGG             CCTGCA^GG
             3969  CCTGCAGG            
Tsp509I       797  AATT                 ^AATT
             2794  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (  133 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        Alw44I      AlwI        AlwNI       ApaI        
ApaLI       AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbeI        BbsI        BbvI        
BcgI        BcnI        BfaI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaHI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrBI       BsrDI       BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DraIII      DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeII       NgoMIV      
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
SmaI        StyI        TaqI        TfiI        TseI        
Tsp45I      TspRI       TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI