################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AocI ApoI AscI AseI AsnI Asp718I AspI BciVI BclI BfrI BglII BsaWI BseAI BsiWI BsmBI BsmI BspDI BspEI BspHI Bst1107I BstBI BstZ17I Bsu36I ClaI DraI Eco81I EcoRI FbaI FseI HindIII HpaI KpnI MfeI MroI MunI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI SalI ScaI SfuI SgrAI SnaBI SpeI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AhdI 4772 GACAAGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 954 AGCGCT AGC^GCT Asp700I 870 GAAGGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 4772 GACAAGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 2482 CCTAGG C^CTAGG BamHI 860 GGATCC G^GATCC BlnI 2482 CCTAGG C^CTAGG BsaBI 2514 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 2200 GAGACC GGTCTC(1/5) BsiEI 26 CGGCCG CG[GA][CT]^CG Bsp1407I 4100 TGTACA T^GTACA BsrGI 4100 TGTACA T^GTACA BssSI 1927 CTCGTG CACGAG(-5/-1) BstEII 1011 GGTCACC G^GT[ACGT]ACC BstPI 1011 GGTCACC G^GT[ACGT]ACC CpoI 4304 CGGACCG CG^G[AT]CCG DrdI 700 GACAACCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 26 CGGCCG C^GGCCG Eam1105I 4772 GACAAGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 26 CGGCCG C^GGCCG Eco47III 954 AGCGCT AGC^GCT Eco52I 26 CGGCCG C^GGCCG EcoNI 392 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1011 GGTCACC G^GT[ACGT]ACC EcoRV 1446 GATATC GAT^ATC HincII 3898 GTCAAC GT[CT]^[GA]AC HindII 3898 GTCAAC GT[CT]^[GA]AC MamI 2514 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 368 ACGCGT A^CGCGT MseI 5121 TTAA T^TAA RsrII 4304 CGGACCG CG^G[AT]CCG SacI 289 GAGCTC GAGCT^C SapI 1683 GCTCTTC GCTCTTC(1/4) SexAI 704 ACCAGGT A^CC[AT]GGT SspBI 4100 TGTACA T^GTACA SspI 5117 AATATT AAT^ATT Tru9I 5121 TTAA T^TAA XmnI 870 GAAGGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AviII 186 TGCGCA TGC^GCA 4346 TGCGCA BbrPI 1272 CACGTG CAC^GTG 4054 CACGTG BbvCI 2253 GCTGAGG CCTCAGC(-5/-2) 2850 GCTGAGG BspLU11I 2810 ACATGT A^CATGT 4586 ACATGT BssHII 349 GCGCGC G^CGCGC 456 GCGCGC EcoT22I 4332 ATGCAT ATGCA^T 4811 ATGCAT FspI 186 TGCGCA TGC^GCA 4346 TGCGCA KspI 135 CCGCGG CCGC^GG 879 CCGCGG NsiI 4332 ATGCAT ATGCA^T 4811 ATGCAT PmaCI 1272 CACGTG CAC^GTG 4054 CACGTG PmlI 1272 CACGTG CAC^GTG 4054 CACGTG PshAI 2315 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3004 GACTGCAGTC SacII 135 CCGCGG CCGC^GG 879 CCGCGG SbfI 1387 CCTGCAGG CCTGCA^GG 3969 CCTGCAGG SfiI 140 GGCCAGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 2227 GGCCTCAGTGGCC SmlI 670 CTCAAG C^T[CT][GA]AG 2506 CTCAAG SphI 2666 GCATGC GCATG^C 2783 GCATGC Sse8387I 1387 CCTGCAGG CCTGCA^GG 3969 CCTGCAGG Tsp509I 797 AATT ^AATT 2794 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 133 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI ApaI ApaLI AspHI AvaI AvaII BalI BanI BanII BbeI BbsI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI