##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
AscI AseI AsnI Asp718I AspEI
AspI BciVI BfrI BglII BsaBI
BsiWI BsrDI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I CpoI
Eam1105I EcoO65I EcoRV EcoT22I HindIII
HpaI KpnI MamI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SapI SbfI SexAI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 74 GTCGAC GT^[AC][GT]AC
AccIII 3334 TCCGGA T^CCGGA
Asp700I 2390 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 3212 CACGTG CAC^GTG
BbvCI 3223 GCTGAGG CCTCAGC(-5/-2)
BcgI 4007 GCACTGTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4211 TGATCA T^GATCA
BsaAI 3212 CACGTG [CT]AC^GT[GA]
BseAI 3334 TCCGGA T^CCGGA
BsiEI 1932 CGGCCG CG[GA][CT]^CG
BsmI 2888 GAATGC GAATGC(1/-1)
Bsp1407I 735 TGTACA T^GTACA
BspDI 2609 ATCGAT AT^CGAT
BspEI 3334 TCCGGA T^CCGGA
BspHI 4932 TCATGA T^CATGA
BspLU11I 261 ACATGT A^CATGT
BsrGI 735 TGTACA T^GTACA
ClaI 2609 ATCGAT AT^CGAT
DraI 4940 TTTAAA TTT^AAA
EagI 1932 CGGCCG C^GGCCG
EclXI 1932 CGGCCG C^GGCCG
Eco52I 1932 CGGCCG C^GGCCG
EcoRI 2649 GAATTC G^AATTC
FbaI 4211 TGATCA T^GATCA
FseI 388 GGCCGGCC GGCCGG^CC
MroI 3334 TCCGGA T^CCGGA
NcoI 4382 CCATGG C^CATGG
PaeR7I 1151 CTCGAG C^TCGAG
PmaCI 3212 CACGTG CAC^GTG
PmlI 3212 CACGTG CAC^GTG
RcaI 4932 TCATGA T^CATGA
SacI 2508 GAGCTC GAGCT^C
SalI 74 GTCGAC G^TCGAC
ScaI 4090 AGTACT AGT^ACT
SspBI 735 TGTACA T^GTACA
XhoI 1151 CTCGAG C^TCGAG
XmnI 2390 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 422 GACGTC GACGT^C
2462 GACGTC
Alw44I 527 GTGCAC G^TGCAC
2171 GTGCAC
Aor51HI 1116 AGCGCT AGC^GCT
3201 AGCGCT
ApaLI 527 GTGCAC G^TGCAC
2171 GTGCAC
AviII 2025 TGCGCA TGC^GCA
2298 TGCGCA
AvrII 2565 CCTAGG C^CTAGG
2836 CCTAGG
BamHI 108 GGATCC G^GATCC
509 GGATCC
BbeI 891 GGCGCC GGCGC^C
2735 GGCGCC
BlnI 2565 CCTAGG C^CTAGG
2836 CCTAGG
BsmBI 2623 CGTCTC CGTCTC(1/5)
1385 GAGACG
BsrBI 2862 CCGCTC CCGCTC(-3/-3)
2354 GAGCGG
BssSI 1193 CACGAG CACGAG(-5/-1)
2174 CACGAG
DrdI 2708 GACGCAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3826 GACATTTGCGTC
Eco47III 1116 AGCGCT AGC^GCT
3201 AGCGCT
FspI 2025 TGCGCA TGC^GCA
2298 TGCGCA
HincII 74 GTCGAC GT[CT]^[GA]AC
2963 GTCAAC
HindII 74 GTCGAC GT[CT]^[GA]AC
2963 GTCAAC
KasI 891 GGCGCC G^GCGCC
2735 GGCGCC
KspI 115 CCGCGG CCGC^GG
4780 CCGCGG
NarI 891 GGCGCC GG^CGCC
2735 GGCGCC
SacII 115 CCGCGG CCGC^GG
4780 CCGCGG
SfiI 959 GGCCCTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4357 GGCCTCCACGGCC
SfoI 891 GGCGCC GGC^GCC
2735 GGCGCC
SmlI 1151 CTCGAG C^T[CT][GA]AG
2341 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI