##################################
# NO CUT ENZYMES (   70 enzymes) #
##################################

AatII       AccI        AclI        AflII       AgeI        
Alw44I      Aor51HI     ApaLI       AscI        AseI        
AsnI        AspI        BbrPI       BfrI        BglII       
BsiEI       BsiWI       BspDI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstZ17I     ClaI        
CpoI        DraI        DrdI        EagI        EclXI       
Eco47III    Eco52I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        HindIII     HpaI        MfeI        
MluI        MunI        NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RsrII       SacI        SalI        SapI        
SbfI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SwaI        TfiI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccIII       2296  TCCGGA               T^CCGGA
AcsI         4400  AAATTC               [GA]^AATT[CT]
AhdI          501  GACCCGCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1718  CCTGAGG              CC^T[ACGT]AGG
ApoI         4400  AAATTC               [GA]^AATT[CT]
Asp700I      4396  GAATAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         501  GACCCGCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        3364  GAGCAC               G[AT]GC[AT]^C
AvrII        3533  CCTAGG               C^CTAGG
BbeI         2733  GGCGCC               GGCGC^C
BciVI        2409  GGATAC               GTATCC(6/5)
BclI         2935  TGATCA               T^GATCA
BlnI         3533  CCTAGG               C^CTAGG
BlpI         3415  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     3415  GCTCAGC              GC^T[ACGT]AGC
BsaAI        1068  TACGTG               [CT]AC^GT[GA]
BsaWI        2296  TCCGGA               [AT]^CCGG[AT]
BseAI        2296  TCCGGA               T^CCGGA
BsiHKAI      3364  GAGCAC               G[AT]GC[AT]^C
BsmI         2506  GCATTC               GAATGC(1/-1)
Bsp1407I     2565  TGTACA               T^GTACA
BspEI        2296  TCCGGA               T^CCGGA
BspHI        3044  TCATGA               T^CATGA
BspLU11I     3018  ACATGT               A^CATGT
BsrDI        1671  GCAATG               GCAATG(2/0)
BsrGI        2565  TGTACA               T^GTACA
BssHII       2456  GCGCGC               G^CGCGC
BssSI         306  CTCGTG               CACGAG(-5/-1)
Bsu36I       1718  CCTGAGG              CC^T[ACGT]AGG
CelII        3415  GCTCAGC              GC^T[ACGT]AGC
DraIII       3937  CACCGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      501  GACCCGCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1238  GAAGAG               CTCTTC(1/4)
Eco81I       1718  CCTGAGG              CC^T[ACGT]AGG
FbaI         2935  TGATCA               T^GATCA
KasI         2733  GGCGCC               G^GCGCC
Ksp632I      1238  GAAGAG               CTCTTC(1/4)
MroI         2296  TCCGGA               T^CCGGA
MseI         3004  TTAA                 T^TAA
NarI         2733  GGCGCC               GG^CGCC
NdeI         3593  CATATG               CA^TATG
PaeR7I       1641  CTCGAG               C^TCGAG
RcaI         3044  TCATGA               T^CATGA
ScaI          973  AGTACT               AGT^ACT
SexAI        1858  ACCTGGT              A^CC[AT]GGT
SfoI         2733  GGCGCC               GGC^GCC
SspBI        2565  TGTACA               T^GTACA
SspI         3822  AATATT               AAT^ATT
Tru9I        3004  TTAA                 T^TAA
XhoI         1641  CTCGAG               C^TCGAG
XmnI         4396  GAATAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

Acc65I       2107  GGTACC               G^GTACC
             2383  GGTACC              
Asp718I      2107  GGTACC               G^GTACC
             2383  GGTACC              
BcgI         1907  CGACCCGGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2186  GCAGCGGCCTCG        
BsaBI        1448  GATCCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1943  GATCCCCATC          
HaeII        2733  GGCGCC               [GA]GCGC^[CT]
             3719  GGCGCT              
HincII        837  GTCAAC               GT[CT]^[GA]AC
             1848  GTTGAC              
HindII        837  GTCAAC               GT[CT]^[GA]AC
             1848  GTTGAC              
KpnI         2107  GGTACC               GGTAC^C
             2383  GGTACC              
KspI         4024  CCGCGG               CCGC^GG
             4096  CCGCGG              
MamI         1448  GATCCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1943  GATCCCCATC          
PstI          284  CTGCAG               CTGCA^G
             3271  CTGCAG              
SacII        4024  CCGCGG               CCGC^GG
             4096  CCGCGG              
SmaI         1346  CCCGGG               CCC^GGG
             3901  CCCGGG              
SphI         1762  GCATGC               GCATG^C
             1786  GCATGC              
XmaCI        1346  CCCGGG               C^CCGGG
             3901  CCCGGG              
XmaI         1346  CCCGGG               C^CCGGG
             3901  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  121 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApaI        AvaI        
AvaII       AviII       BalI        BamHI       BanI        
BanII       BbsI        BbvCI       BbvI        BcnI        
BfaI        BglI        BmyI        BpmI        BpuAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        FspI        HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NciI        NcoI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PshAI       PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfiI        SmlI        
StuI        StyI        TaqI        TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoII