################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AatII AccI AclI AflII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI AspI BbrPI BfrI BglII BsiEI BsiWI BspDI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI HindIII HpaI MfeI MluI MunI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SalI SapI SbfI SfuI SgrAI SnaBI SpeI Sse8387I SwaI TfiI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccIII 2296 TCCGGA T^CCGGA AcsI 4400 AAATTC [GA]^AATT[CT] AhdI 501 GACCCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1718 CCTGAGG CC^T[ACGT]AGG ApoI 4400 AAATTC [GA]^AATT[CT] Asp700I 4396 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 501 GACCCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 3364 GAGCAC G[AT]GC[AT]^C AvrII 3533 CCTAGG C^CTAGG BbeI 2733 GGCGCC GGCGC^C BciVI 2409 GGATAC GTATCC(6/5) BclI 2935 TGATCA T^GATCA BlnI 3533 CCTAGG C^CTAGG BlpI 3415 GCTCAGC GC^T[ACGT]AGC Bpu1102I 3415 GCTCAGC GC^T[ACGT]AGC BsaAI 1068 TACGTG [CT]AC^GT[GA] BsaWI 2296 TCCGGA [AT]^CCGG[AT] BseAI 2296 TCCGGA T^CCGGA BsiHKAI 3364 GAGCAC G[AT]GC[AT]^C BsmI 2506 GCATTC GAATGC(1/-1) Bsp1407I 2565 TGTACA T^GTACA BspEI 2296 TCCGGA T^CCGGA BspHI 3044 TCATGA T^CATGA BspLU11I 3018 ACATGT A^CATGT BsrDI 1671 GCAATG GCAATG(2/0) BsrGI 2565 TGTACA T^GTACA BssHII 2456 GCGCGC G^CGCGC BssSI 306 CTCGTG CACGAG(-5/-1) Bsu36I 1718 CCTGAGG CC^T[ACGT]AGG CelII 3415 GCTCAGC GC^T[ACGT]AGC DraIII 3937 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 501 GACCCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1238 GAAGAG CTCTTC(1/4) Eco81I 1718 CCTGAGG CC^T[ACGT]AGG FbaI 2935 TGATCA T^GATCA KasI 2733 GGCGCC G^GCGCC Ksp632I 1238 GAAGAG CTCTTC(1/4) MroI 2296 TCCGGA T^CCGGA MseI 3004 TTAA T^TAA NarI 2733 GGCGCC GG^CGCC NdeI 3593 CATATG CA^TATG PaeR7I 1641 CTCGAG C^TCGAG RcaI 3044 TCATGA T^CATGA ScaI 973 AGTACT AGT^ACT SexAI 1858 ACCTGGT A^CC[AT]GGT SfoI 2733 GGCGCC GGC^GCC SspBI 2565 TGTACA T^GTACA SspI 3822 AATATT AAT^ATT Tru9I 3004 TTAA T^TAA XhoI 1641 CTCGAG C^TCGAG XmnI 4396 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### Acc65I 2107 GGTACC G^GTACC 2383 GGTACC Asp718I 2107 GGTACC G^GTACC 2383 GGTACC BcgI 1907 CGACCCGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2186 GCAGCGGCCTCG BsaBI 1448 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1943 GATCCCCATC HaeII 2733 GGCGCC [GA]GCGC^[CT] 3719 GGCGCT HincII 837 GTCAAC GT[CT]^[GA]AC 1848 GTTGAC HindII 837 GTCAAC GT[CT]^[GA]AC 1848 GTTGAC KpnI 2107 GGTACC GGTAC^C 2383 GGTACC KspI 4024 CCGCGG CCGC^GG 4096 CCGCGG MamI 1448 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1943 GATCCCCATC PstI 284 CTGCAG CTGCA^G 3271 CTGCAG SacII 4024 CCGCGG CCGC^GG 4096 CCGCGG SmaI 1346 CCCGGG CCC^GGG 3901 CCCGGG SphI 1762 GCATGC GCATG^C 1786 GCATGC XmaCI 1346 CCCGGG C^CCGGG 3901 CCCGGG XmaI 1346 CCCGGG C^CCGGG 3901 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI ApaI AvaI AvaII AviII BalI BamHI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PshAI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SmlI StuI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII