##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AccI AccIII AclI AgeI AhdI
AscI AseI AsnI AspEI AviII
BbrPI BcgI BclI BsaAI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BsrGI Bst1107I BstBI BstZ17I
ClaI CpoI EagI Eam1105I EclXI
Eco52I EcoNI EcoRI EcoRV FbaI
FseI FspI KspI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI XbaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 4189 GACGTC GACGT^C
Acc65I 2503 GGTACC G^GTACC
AflII 2668 CTTAAG C^TTAAG
AflIII 1673 ACATGT A^C[GA][CT]GT
Aor51HI 4305 AGCGCT AGC^GCT
Asp700I 3980 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2503 GGTACC G^GTACC
AvrII 1455 CCTAGG C^CTAGG
BamHI 141 GGATCC G^GATCC
BbvCI 1984 GCTGAGG CCTCAGC(-5/-2)
BciVI 5018 GTATCC GTATCC(6/5)
BfrI 2668 CTTAAG C^TTAAG
BglII 3475 AGATCT A^GATCT
BlnI 1455 CCTAGG C^CTAGG
BsaBI 2473 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 4258 CGACCG CG[GA][CT]^CG
BspLU11I 1673 ACATGT A^CATGT
BsrBI 3725 GAGCGG CCGCTC(-3/-3)
BssHII 4360 GCGCGC G^CGCGC
BstEII 149 GGTAACC G^GT[ACGT]ACC
BstPI 149 GGTAACC G^GT[ACGT]ACC
DraIII 2658 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 182 GACTCCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4305 AGCGCT AGC^GCT
EcoO65I 149 GGTAACC G^GT[ACGT]ACC
HpaI 129 GTTAAC GTT^AAC
KpnI 2503 GGTACC GGTAC^C
MamI 2473 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 4863 GCCGGC GCC^GGC
NcoI 234 CCATGG C^CATGG
NgoMIV 4863 GCCGGC G^CCGGC
PaeR7I 4503 CTCGAG C^TCGAG
SapI 3483 GCTCTTC GCTCTTC(1/4)
ScaI 2836 AGTACT AGT^ACT
SwaI 1715 ATTTAAAT ATTT^AAAT
XhoI 4503 CTCGAG C^TCGAG
XmnI 3980 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 984 GTGCAC G^TGCAC
3156 GTGCAC
AocI 1131 CCTGAGG CC^T[ACGT]AGG
5056 CCTTAGG
ApaLI 984 GTGCAC G^TGCAC
3156 GTGCAC
AspI 5 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4631 GACCGTGTC
BbeI 1240 GGCGCC GGCGC^C
1956 GGCGCC
BbsI 2755 GAAGAC GAAGAC(2/6)
3928 GAAGAC
BglI 1232 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3591 GCCTGGGCGGC
BpuAI 2755 GAAGAC GAAGAC(2/6)
3928 GAAGAC
BsrFI 4260 ACCGGC [GA]^CCGG[CT]
4863 GCCGGC
Bsu36I 1131 CCTGAGG CC^T[ACGT]AGG
5056 CCTTAGG
Cfr10I 4260 ACCGGC [GA]^CCGG[CT]
4863 GCCGGC
Eco81I 1131 CCTGAGG CC^T[ACGT]AGG
5056 CCTTAGG
HgaI 3341 GACGC GACGC(5/10)
4583 GCGTC
KasI 1240 GGCGCC G^GCGCC
1956 GGCGCC
NarI 1240 GGCGCC GG^CGCC
1956 GGCGCC
PflFI 5 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4631 GACCGTGTC
PshAI 1555 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3014 GACATCTGTC
SbfI 4081 CCTGCAGG CCTGCA^GG
4588 CCTGCAGG
SfoI 1240 GGCGCC GGC^GCC
1956 GGCGCC
Sse8387I 4081 CCTGCAGG CCTGCA^GG
4588 CCTGCAGG
Tth111I 5 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4631 GACCGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrDI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI