################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AgeI Aor51HI AscI Asp718I AspI AviII BcgI BciVI BclI BglII BseAI BsiWI BsmBI BsmI BspDI BspEI BssHII Bst1107I BstAPI BstZ17I ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI MfeI MluI MroI MunI NaeI NgoMIV NheI NotI NruI NsiI PacI PflFI PinAI PmeI PshAI Psp1406I RsrII SalI SapI SbfI SfiI SgrAI SnaBI SpeI SphI Sse8387I SwaI Tth111I ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AflII 3674 CTTAAG C^TTAAG AhdI 1992 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 510 GTGCAC G^TGCAC ApaLI 510 GTGCAC G^TGCAC AseI 3476 ATTAAT AT^TAAT AsnI 3476 ATTAAT AT^TAAT AspEI 1992 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 3013 CCTAGG C^CTAGG BamHI 1950 GGATCC G^GATCC BbrPI 168 CACGTG CAC^GTG BfrI 3674 CTTAAG C^TTAAG BlnI 3013 CCTAGG C^CTAGG BsaAI 168 CACGTG [CT]AC^GT[GA] BsaBI 932 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 2880 GGTCTC GGTCTC(1/5) BsaWI 3397 ACCGGA [AT]^CCGG[AT] BsiEI 38 CGATCG CG[GA][CT]^CG BspLU11I 838 ACATGT A^CATGT BsrDI 3760 CATTGC GCAATG(2/0) BsrFI 1732 ACCGGC [GA]^CCGG[CT] BstBI 2219 TTCGAA TT^CGAA BstEII 1883 GGTTACC G^GT[ACGT]ACC BstPI 1883 GGTTACC G^GT[ACGT]ACC Cfr10I 1732 ACCGGC [GA]^CCGG[CT] DraIII 115 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1992 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 1883 GGTTACC G^GT[ACGT]ACC EcoRI 3788 GAATTC G^AATTC KspI 1387 CCGCGG CCGC^GG MamI 932 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NdeI 664 CATATG CA^TATG NspV 2219 TTCGAA TT^CGAA PflMI 3072 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 168 CACGTG CAC^GTG PmlI 168 CACGTG CAC^GTG PshBI 3476 ATTAAT AT^TAAT PvuI 38 CGATCG CGAT^CG SacII 1387 CCGCGG CCGC^GG ScaI 806 AGTACT AGT^ACT SfuI 2219 TTCGAA TT^CGAA SspI 531 AATATT AAT^ATT StuI 2686 AGGCCT AGG^CCT Van91I 3072 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AflIII 643 ACACGT A^C[GA][CT]GT 838 ACATGT Asp700I 308 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1192 GAACAAGTTC BalI 1718 TGGCCA TGG^CCA 3989 TGGCCA BbvCI 4379 CCTCAGC CCTCAGC(-5/-2) 188 GCTGAGG BspMI 2957 ACCTGC ACCTGC(4/8) 3038 ACCTGC BssSI 1487 CACGAG CACGAG(-5/-1) 444 CTCGTG HgaI 392 GACGC GACGC(5/10) 4555 GCGTC HincII 842 GTCAAC GT[CT]^[GA]AC 4689 GTTGAC HindII 842 GTCAAC GT[CT]^[GA]AC 4689 GTTGAC MluNI 1718 TGGCCA TGG^CCA 3989 TGGCCA MscI 1718 TGGCCA TGG^CCA 3989 TGGCCA MslI 2183 CACGGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 4835 CACTGGCGTG NspI 838 ACATGT [GA]CATG^[CT] 4507 GCATGT PvuII 285 CAGCTG CAG^CTG 2237 CAGCTG SacI 2336 GAGCTC GAGCT^C 4292 GAGCTC SexAI 400 ACCTGGT A^CC[AT]GGT 4785 ACCTGGT SmaI 29 CCCGGG CCC^GGG 1556 CCCGGG XbaI 3467 TCTAGA T^CTAGA 3982 TCTAGA XmaCI 29 CCCGGG C^CCGGG 1556 CCCGGG XmaI 29 CCCGGG C^CCGGG 1556 CCCGGG XmnI 308 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1192 GAACAAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BanI BanII BbeI BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspHI BsrBI BsrGI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV PaeR7I PleI PpuMI PstI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoI XhoII