##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AclI AgeI AhdI Alw44I
ApaLI AscI AspEI AspI AviII
BbrPI BcgI BlpI Bpu1102I BsiWI
BsrBI BssHII BstBI CelII EagI
Eam1105I EclXI Eco52I EcoRV FseI
FspI HindIII MfeI MluI MunI
NaeI NgoMIV NheI NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I SalI
SbfI SfuI SgrAI SmaI SnaBI
Sse8387I Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 69 GGTACC G^GTACC
AccIII 2606 TCCGGA T^CCGGA
AflII 6304 CTTAAG C^TTAAG
Aor51HI 3153 AGCGCT AGC^GCT
ApaI 6764 GGGCCC GGGCC^C
Asp718I 69 GGTACC G^GTACC
BbeI 2833 GGCGCC GGCGC^C
BclI 3015 TGATCA T^GATCA
BfrI 6304 CTTAAG C^TTAAG
BsaBI 2352 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2606 TCCGGA [AT]^CCGG[AT]
BseAI 2606 TCCGGA T^CCGGA
BsiEI 1421 CGATCG CG[GA][CT]^CG
BspEI 2606 TCCGGA T^CCGGA
Bst1107I 1982 GTATAC GTA^TAC
BstEII 2189 GGTTACC G^GT[ACGT]ACC
BstPI 2189 GGTTACC G^GT[ACGT]ACC
BstZ17I 1982 GTATAC GTA^TAC
CpoI 3097 CGGACCG CG^G[AT]CCG
DraIII 5186 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 3153 AGCGCT AGC^GCT
EcoO65I 2189 GGTTACC G^GT[ACGT]ACC
FbaI 3015 TGATCA T^GATCA
KasI 2833 GGCGCC G^GCGCC
KpnI 69 GGTACC GGTAC^C
KspI 2695 CCGCGG CCGC^GG
MamI 2352 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2606 TCCGGA T^CCGGA
NarI 2833 GGCGCC GG^CGCC
NcoI 5682 CCATGG C^CATGG
NdeI 4744 CATATG CA^TATG
PaeR7I 6222 CTCGAG C^TCGAG
PpuMI 2110 AGGTCCC [GA]G^G[AT]CC[CT]
PvuI 1421 CGATCG CGAT^CG
RsrII 3097 CGGACCG CG^G[AT]CCG
SacII 2695 CCGCGG CCGC^GG
SexAI 1617 ACCAGGT A^CC[AT]GGT
SfiI 6313 GGCCTGAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2833 GGCGCC GGC^GCC
SpeI 5876 ACTAGT A^CTAGT
XbaI 5584 TCTAGA T^CTAGA
XhoI 6222 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 2833 GGCGCC G[GA]^CG[CT]C
3373 GGCGTC
AseI 5295 ATTAAT AT^TAAT
5765 ATTAAT
AsnI 5295 ATTAAT AT^TAAT
5765 ATTAAT
AvrII 5321 CCTAGG C^CTAGG
6528 CCTAGG
BbsI 1232 GAAGAC GAAGAC(2/6)
3551 GAAGAC
BbvCI 2194 CCTCAGC CCTCAGC(-5/-2)
3033 CCTCAGC
BciVI 3076 GGATAC GTATCC(6/5)
361 GTATCC
BglI 4625 GCCAGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
6314 GCCTGAAAGGC
BlnI 5321 CCTAGG C^CTAGG
6528 CCTAGG
BpuAI 1232 GAAGAC GAAGAC(2/6)
3551 GAAGAC
BsaAI 3481 TACGTG [CT]AC^GT[GA]
5304 CACGTA
BsaHI 2833 GGCGCC G[GA]^CG[CT]C
3373 GGCGTC
BsmBI 2818 CGTCTC CGTCTC(1/5)
647 GAGACG
BsmI 1430 GCATTC GAATGC(1/-1)
4297 GCATTC
BspDI 1423 ATCGAT AT^CGAT
3199 ATCGAT
BspHI 3129 TCATGA T^CATGA
6645 TCATGA
BsrFI 306 ACCGGC [GA]^CCGG[CT]
2919 GCCGGT
BssSI 4591 CACGAG CACGAG(-5/-1)
2821 CTCGTG
Cfr10I 306 ACCGGC [GA]^CCGG[CT]
2919 GCCGGT
ClaI 1423 ATCGAT AT^CGAT
3199 ATCGAT
DrdI 3210 GACCTCGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3391 GACCCAGGTGTC
DsaI 2695 CCGCGG C^C[GA][CT]GG
5682 CCATGG
EcoNI 838 CCTTTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
6604 CCTCATAAAGG
EcoT22I 3134 ATGCAT ATGCA^T
3754 ATGCAT
HaeII 2833 GGCGCC [GA]GCGC^[CT]
3153 AGCGCT
Hin1I 2833 GGCGCC G[GA]^CG[CT]C
3373 GGCGTC
NsiI 3134 ATGCAT ATGCA^T
3754 ATGCAT
PflMI 1734 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4648 CCATCACATGG
PshBI 5295 ATTAAT AT^TAAT
5765 ATTAAT
RcaI 3129 TCATGA T^CATGA
6645 TCATGA
ScaI 4917 AGTACT AGT^ACT
7172 AGTACT
SphI 2261 GCATGC GCATG^C
6561 GCATGC
SwaI 5635 ATTTAAAT ATTT^AAAT
5693 ATTTAAAT
Van91I 1734 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4648 CCATCACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI Asp700I AspHI AvaI AvaII
BalI BamHI BanI BanII BbvI
BcnI BfaI BglII BmyI BpmI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspLU11I BspMI BsrDI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EaeI EarI Eco81I
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI