##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspI AvaI AviII
BanI BbeI BbrPI BcgI BcnI
BglI BsaAI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsoBI BspDI
BspEI BsrFI BssHII BssSI BstUI
Cfr10I ClaI CpoI DsaI EagI
EarI EclXI Eco47III Eco52I FseI
FspI HapII HgaI Hin1I HpaI
HpaII KasI KpnI Ksp632I KspI
MluI MroI MspI MvnI NaeI
NarI NciI NcoI NgoMIV NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PvuI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AflII 3910 CTTAAG C^TTAAG
Asp700I 3876 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 4519 CCTAGG C^CTAGG
BalI 215 TGGCCA TGG^CCA
BbvCI 1294 CCTCAGC CCTCAGC(-5/-2)
BfrI 3910 CTTAAG C^TTAAG
BlnI 4519 CCTAGG C^CTAGG
BlpI 262 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 262 GCTCAGC GC^T[ACGT]AGC
BsaBI 1894 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 5306 CGTCTC CGTCTC(1/5)
BsrBI 522 GAGCGG CCGCTC(-3/-3)
Bst1107I 1202 GTATAC GTA^TAC
BstBI 348 TTCGAA TT^CGAA
BstXI 3736 CCAAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1202 GTATAC GTA^TAC
CelII 262 GCTCAGC GC^T[ACGT]AGC
DraII 4031 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 128 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3139 GACTTTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 215 TGGCCA [CT]^GGCC[GA]
EcoNI 4364 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 4031 AGGTCCT [GA]G^G[ACGT]CC[CT]
FauI 1680 CCCGC CCCGC(4/6)
HaeII 4381 GGCGCT [GA]GCGC^[CT]
MamI 1894 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3150 CAATTG C^AATTG
MluNI 215 TGGCCA TGG^CCA
MscI 215 TGGCCA TGG^CCA
MunI 3150 CAATTG C^AATTG
NheI 2310 GCTAGC G^CTAGC
NspV 348 TTCGAA TT^CGAA
PflMI 1753 CCATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 4031 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 814 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 185 GAGCTC GAGCT^C
SfuI 348 TTCGAA TT^CGAA
SphI 4770 GCATGC GCATG^C
StuI 3749 AGGCCT AGG^CCT
Van91I 1753 CCATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3135 TCTAGA T^CTAGA
XmnI 3876 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AclI 3435 AACGTT AA^CGTT
4638 AACGTT
AflIII 681 ACATGT A^C[GA][CT]GT
3419 ACATGT
AhdI 1417 GACGTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2448 GACTCAAAGTC
AseI 4694 ATTAAT AT^TAAT
5267 ATTAAT
AsnI 4694 ATTAAT AT^TAAT
5267 ATTAAT
AspEI 1417 GACGTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2448 GACTCAAAGTC
BamHI 1762 GGATCC G^GATCC
4373 GGATCC
BanII 185 GAGCTC G[GA]GC[CT]^C
4535 GAGCCC
BbsI 2049 GTCTTC GAAGAC(2/6)
4659 GTCTTC
BglII 2683 AGATCT A^GATCT
3027 AGATCT
BpuAI 2049 GTCTTC GAAGAC(2/6)
4659 GTCTTC
BsmI 48 GAATGC GAATGC(1/-1)
1444 GAATGC
BspHI 687 TCATGA T^CATGA
5000 TCATGA
BspLU11I 681 ACATGT A^CATGT
3419 ACATGT
BstAPI 577 GCATCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3539 GCATTATGTGC
BstEII 426 GGTAACC G^GT[ACGT]ACC
3969 GGTCACC
BstPI 426 GGTAACC G^GT[ACGT]ACC
3969 GGTCACC
CfoI 1562 GCGC GCG^C
4382 GCGC
Eam1105I 1417 GACGTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2448 GACTCAAAGTC
EcoO65I 426 GGTAACC G^GT[ACGT]ACC
3969 GGTCACC
EcoT14I 405 CCAAGG C^C[AT][AT]GG
4519 CCTAGG
EcoT22I 775 ATGCAT ATGCA^T
3537 ATGCAT
HhaI 1562 GCGC GCG^C
4382 GCGC
HinP1I 1562 GCGC G^CGC
4382 GCGC
HincII 2655 GTCAAC GT[CT]^[GA]AC
4563 GTTGAC
HindII 2655 GTCAAC GT[CT]^[GA]AC
4563 GTTGAC
NdeI 1661 CATATG CA^TATG
4571 CATATG
NsiI 775 ATGCAT ATGCA^T
3537 ATGCAT
PshBI 4694 ATTAAT AT^TAAT
5267 ATTAAT
Psp1406I 3435 AACGTT AA^CGTT
4638 AACGTT
RcaI 687 TCATGA T^CATGA
5000 TCATGA
StyI 405 CCAAGG C^C[AT][AT]GG
4519 CCTAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaII BbvI BciVI BclI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspMI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraI Eco81I EcoRI EcoRII EcoRV
FbaI Fnu4HI FokI HaeIII HindIII
HinfI HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
SspBI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII