##################################
# NO CUT ENZYMES (   92 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcyI        
AgeI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        Asp718I     AspI        AvaI        AviII       
BanI        BbeI        BbrPI       BcgI        BcnI        
BglI        BsaAI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsoBI       BspDI       
BspEI       BsrFI       BssHII      BssSI       BstUI       
Cfr10I      ClaI        CpoI        DsaI        EagI        
EarI        EclXI       Eco47III    Eco52I      FseI        
FspI        HapII       HgaI        Hin1I       HpaI        
HpaII       KasI        KpnI        Ksp632I     KspI        
MluI        MroI        MspI        MvnI        NaeI        
NarI        NciI        NcoI        NgoMIV      NotI        
NruI        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PvuI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SwaI        Tth111I     XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AflII        3910  CTTAAG               C^TTAAG
Asp700I      3876  GAACATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII        4519  CCTAGG               C^CTAGG
BalI          215  TGGCCA               TGG^CCA
BbvCI        1294  CCTCAGC              CCTCAGC(-5/-2)
BfrI         3910  CTTAAG               C^TTAAG
BlnI         4519  CCTAGG               C^CTAGG
BlpI          262  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      262  GCTCAGC              GC^T[ACGT]AGC
BsaBI        1894  GATGTATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI        5306  CGTCTC               CGTCTC(1/5)
BsrBI         522  GAGCGG               CCGCTC(-3/-3)
Bst1107I     1202  GTATAC               GTA^TAC
BstBI         348  TTCGAA               TT^CGAA
BstXI        3736  CCAAAAATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1202  GTATAC               GTA^TAC
CelII         262  GCTCAGC              GC^T[ACGT]AGC
DraII        4031  AGGTCCT              [GA]G^G[ACGT]CC[CT]
DraIII        128  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         3139  GACTTTCCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          215  TGGCCA               [CT]^GGCC[GA]
EcoNI        4364  CCTGAAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     4031  AGGTCCT              [GA]G^G[ACGT]CC[CT]
FauI         1680  CCCGC                CCCGC(4/6)
HaeII        4381  GGCGCT               [GA]GCGC^[CT]
MamI         1894  GATGTATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         3150  CAATTG               C^AATTG
MluNI         215  TGGCCA               TGG^CCA
MscI          215  TGGCCA               TGG^CCA
MunI         3150  CAATTG               C^AATTG
NheI         2310  GCTAGC               G^CTAGC
NspV          348  TTCGAA               TT^CGAA
PflMI        1753  CCATTCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        4031  AGGTCCT              [GA]G^G[AT]CC[CT]
PshAI         814  GACTCAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI          185  GAGCTC               GAGCT^C
SfuI          348  TTCGAA               TT^CGAA
SphI         4770  GCATGC               GCATG^C
StuI         3749  AGGCCT               AGG^CCT
Van91I       1753  CCATTCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         3135  TCTAGA               T^CTAGA
XmnI         3876  GAACATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AclI         3435  AACGTT               AA^CGTT
             4638  AACGTT              
AflIII        681  ACATGT               A^C[GA][CT]GT
             3419  ACATGT              
AhdI         1417  GACGTTCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2448  GACTCAAAGTC         
AseI         4694  ATTAAT               AT^TAAT
             5267  ATTAAT              
AsnI         4694  ATTAAT               AT^TAAT
             5267  ATTAAT              
AspEI        1417  GACGTTCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2448  GACTCAAAGTC         
BamHI        1762  GGATCC               G^GATCC
             4373  GGATCC              
BanII         185  GAGCTC               G[GA]GC[CT]^C
             4535  GAGCCC              
BbsI         2049  GTCTTC               GAAGAC(2/6)
             4659  GTCTTC              
BglII        2683  AGATCT               A^GATCT
             3027  AGATCT              
BpuAI        2049  GTCTTC               GAAGAC(2/6)
             4659  GTCTTC              
BsmI           48  GAATGC               GAATGC(1/-1)
             1444  GAATGC              
BspHI         687  TCATGA               T^CATGA
             5000  TCATGA              
BspLU11I      681  ACATGT               A^CATGT
             3419  ACATGT              
BstAPI        577  GCATCTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3539  GCATTATGTGC         
BstEII        426  GGTAACC              G^GT[ACGT]ACC
             3969  GGTCACC             
BstPI         426  GGTAACC              G^GT[ACGT]ACC
             3969  GGTCACC             
CfoI         1562  GCGC                 GCG^C
             4382  GCGC                
Eam1105I     1417  GACGTTCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2448  GACTCAAAGTC         
EcoO65I       426  GGTAACC              G^GT[ACGT]ACC
             3969  GGTCACC             
EcoT14I       405  CCAAGG               C^C[AT][AT]GG
             4519  CCTAGG              
EcoT22I       775  ATGCAT               ATGCA^T
             3537  ATGCAT              
HhaI         1562  GCGC                 GCG^C
             4382  GCGC                
HinP1I       1562  GCGC                 G^CGC
             4382  GCGC                
HincII       2655  GTCAAC               GT[CT]^[GA]AC
             4563  GTTGAC              
HindII       2655  GTCAAC               GT[CT]^[GA]AC
             4563  GTTGAC              
NdeI         1661  CATATG               CA^TATG
             4571  CATATG              
NsiI          775  ATGCAT               ATGCA^T
             3537  ATGCAT              
PshBI        4694  ATTAAT               AT^TAAT
             5267  ATTAAT              
Psp1406I     3435  AACGTT               AA^CGTT
             4638  AACGTT              
RcaI          687  TCATGA               T^CATGA
             5000  TCATGA              
StyI          405  CCAAGG               C^C[AT][AT]GG
             4519  CCTAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   92 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       AocI        ApoI        AspHI       
AvaII       BbvI        BciVI       BclI        BfaI        
BmyI        BpmI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    Bsp1407I    BspMI       BsrDI       BsrGI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Bsu36I      Cac8I       Cfr13I      DdeI        DpnII       
DraI        Eco81I      EcoRI       EcoRII      EcoRV       
FbaI        Fnu4HI      FokI        HaeIII      HindIII     
HinfI       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MvaI        MwoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SexAI       SfaNI       SfcI        SmlI        
SspBI       SspI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        XhoII