################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII Acc65I AccII AccIII AcyI AgeI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspI AvaI AviII BanI BbeI BbrPI BcgI BcnI BglI BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsoBI BspDI BspEI BsrFI BssHII BssSI BstUI Cfr10I ClaI CpoI DsaI EagI EarI EclXI Eco47III Eco52I FseI FspI HapII HgaI Hin1I HpaI HpaII KasI KpnI Ksp632I KspI MluI MroI MspI MvnI NaeI NarI NciI NcoI NgoMIV NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AflII 3910 CTTAAG C^TTAAG Asp700I 3876 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 4519 CCTAGG C^CTAGG BalI 215 TGGCCA TGG^CCA BbvCI 1294 CCTCAGC CCTCAGC(-5/-2) BfrI 3910 CTTAAG C^TTAAG BlnI 4519 CCTAGG C^CTAGG BlpI 262 GCTCAGC GC^T[ACGT]AGC Bpu1102I 262 GCTCAGC GC^T[ACGT]AGC BsaBI 1894 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 5306 CGTCTC CGTCTC(1/5) BsrBI 522 GAGCGG CCGCTC(-3/-3) Bst1107I 1202 GTATAC GTA^TAC BstBI 348 TTCGAA TT^CGAA BstXI 3736 CCAAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1202 GTATAC GTA^TAC CelII 262 GCTCAGC GC^T[ACGT]AGC DraII 4031 AGGTCCT [GA]G^G[ACGT]CC[CT] DraIII 128 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 3139 GACTTTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 215 TGGCCA [CT]^GGCC[GA] EcoNI 4364 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 4031 AGGTCCT [GA]G^G[ACGT]CC[CT] FauI 1680 CCCGC CCCGC(4/6) HaeII 4381 GGCGCT [GA]GCGC^[CT] MamI 1894 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3150 CAATTG C^AATTG MluNI 215 TGGCCA TGG^CCA MscI 215 TGGCCA TGG^CCA MunI 3150 CAATTG C^AATTG NheI 2310 GCTAGC G^CTAGC NspV 348 TTCGAA TT^CGAA PflMI 1753 CCATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 4031 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 814 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 185 GAGCTC GAGCT^C SfuI 348 TTCGAA TT^CGAA SphI 4770 GCATGC GCATG^C StuI 3749 AGGCCT AGG^CCT Van91I 1753 CCATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 3135 TCTAGA T^CTAGA XmnI 3876 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AclI 3435 AACGTT AA^CGTT 4638 AACGTT AflIII 681 ACATGT A^C[GA][CT]GT 3419 ACATGT AhdI 1417 GACGTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2448 GACTCAAAGTC AseI 4694 ATTAAT AT^TAAT 5267 ATTAAT AsnI 4694 ATTAAT AT^TAAT 5267 ATTAAT AspEI 1417 GACGTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2448 GACTCAAAGTC BamHI 1762 GGATCC G^GATCC 4373 GGATCC BanII 185 GAGCTC G[GA]GC[CT]^C 4535 GAGCCC BbsI 2049 GTCTTC GAAGAC(2/6) 4659 GTCTTC BglII 2683 AGATCT A^GATCT 3027 AGATCT BpuAI 2049 GTCTTC GAAGAC(2/6) 4659 GTCTTC BsmI 48 GAATGC GAATGC(1/-1) 1444 GAATGC BspHI 687 TCATGA T^CATGA 5000 TCATGA BspLU11I 681 ACATGT A^CATGT 3419 ACATGT BstAPI 577 GCATCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3539 GCATTATGTGC BstEII 426 GGTAACC G^GT[ACGT]ACC 3969 GGTCACC BstPI 426 GGTAACC G^GT[ACGT]ACC 3969 GGTCACC CfoI 1562 GCGC GCG^C 4382 GCGC Eam1105I 1417 GACGTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2448 GACTCAAAGTC EcoO65I 426 GGTAACC G^GT[ACGT]ACC 3969 GGTCACC EcoT14I 405 CCAAGG C^C[AT][AT]GG 4519 CCTAGG EcoT22I 775 ATGCAT ATGCA^T 3537 ATGCAT HhaI 1562 GCGC GCG^C 4382 GCGC HinP1I 1562 GCGC G^CGC 4382 GCGC HincII 2655 GTCAAC GT[CT]^[GA]AC 4563 GTTGAC HindII 2655 GTCAAC GT[CT]^[GA]AC 4563 GTTGAC NdeI 1661 CATATG CA^TATG 4571 CATATG NsiI 775 ATGCAT ATGCA^T 3537 ATGCAT PshBI 4694 ATTAAT AT^TAAT 5267 ATTAAT Psp1406I 3435 AACGTT AA^CGTT 4638 AACGTT RcaI 687 TCATGA T^CATGA 5000 TCATGA StyI 405 CCAAGG C^C[AT][AT]GG 4519 CCTAGG ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaII BbvI BciVI BclI BfaI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraI Eco81I EcoRI EcoRII EcoRV FbaI Fnu4HI FokI HaeIII HindIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI SspBI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII