##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AclI AgeI AhdI Aor51HI
AscI AseI AsnI AspEI AviII
AvrII BamHI BbrPI BbvCI BcgI
BlnI BsaAI BsaBI BsiEI BsiWI
BspDI BsrFI BssHII BssSI Bst1107I
BstBI BstZ17I Cfr10I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRV FseI FspI HpaI
MamI MfeI MluI MunI NaeI
NgoMIV NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacI
SalI SbfI ScaI SfiI SfuI
SgrAI SmaI SnaBI Sse8387I SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 4449 GGTACC G^GTACC
AccII 23 CGCG CG^CG
AccIII 4725 TCCGGA T^CCGGA
AcyI 18 GGCGCC G[GA]^CG[CT]C
AflII 3192 CTTAAG C^TTAAG
Asp718I 4449 GGTACC G^GTACC
AspI 2877 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2755 CCCGAG C^[CT]CG[GA]G
BbeI 18 GGCGCC GGCGC^C
BfrI 3192 CTTAAG C^TTAAG
BglII 1257 AGATCT A^GATCT
BlpI 960 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 960 GCTGAGC GC^T[ACGT]AGC
BsaHI 18 GGCGCC G[GA]^CG[CT]C
BseAI 4725 TCCGGA T^CCGGA
BsoBI 2755 CCCGAG C^[CT]CG[GA]G
BspEI 4725 TCCGGA T^CCGGA
BspLU11I 2114 ACATGT A^CATGT
BsrBI 1644 GAGCGG CCGCTC(-3/-3)
BstUI 23 CGCG CG^CG
CelII 960 GCTGAGC GC^T[ACGT]AGC
Hin1I 18 GGCGCC G[GA]^CG[CT]C
HincII 1324 GTCAAC GT[CT]^[GA]AC
HindII 1324 GTCAAC GT[CT]^[GA]AC
KasI 18 GGCGCC G^GCGCC
KpnI 4449 GGTACC GGTAC^C
KspI 22 CCGCGG CCGC^GG
MroI 4725 TCCGGA T^CCGGA
MslI 4550 CATAAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 23 CGCG CG^CG
NarI 18 GGCGCC GG^CGCC
NcoI 4318 CCATGG C^CATGG
NheI 5275 GCTAGC G^CTAGC
PflFI 2877 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 3802 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 4577 GACCGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1413 CTGCAG CTGCA^G
SacII 22 CCGCGG CCGC^GG
SapI 5179 GCTCTTC GCTCTTC(1/4)
SexAI 2417 ACCAGGT A^CC[AT]GGT
SfoI 18 GGCGCC GGC^GCC
SphI 2924 GCATGC GCATG^C
SspI 3242 AATATT AAT^ATT
Tth111I 2877 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4073 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccI 1692 GTAGAC GT^[AC][GT]AC
2307 GTCTAC
AflIII 324 ACGTGT A^C[GA][CT]GT
2114 ACATGT
Alw44I 496 GTGCAC G^TGCAC
3486 GTGCAC
AocI 1660 CCTCAGG CC^T[ACGT]AGG
4259 CCTCAGG
ApaI 4393 GGGCCC GGGCC^C
4854 GGGCCC
ApaLI 496 GTGCAC G^TGCAC
3486 GTGCAC
BalI 2726 TGGCCA TGG^CCA
4650 TGGCCA
BciVI 680 GTATCC GTATCC(6/5)
1425 GTATCC
BclI 1972 TGATCA T^GATCA
4882 TGATCA
BglI 4856 GCCCACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5121 GCCACTCTGGC
BsaWI 4578 ACCGGA [AT]^CCGG[AT]
4725 TCCGGA
BsmBI 775 CGTCTC CGTCTC(1/5)
5032 GAGACG
Bsp1407I 1037 TGTACA T^GTACA
3637 TGTACA
BsrGI 1037 TGTACA T^GTACA
3637 TGTACA
BstXI 4383 CCAATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4898 CCACTCATTTGG
BstYI 1257 AGATCT [GA]^GATC[CT]
4000 GGATCT
Bsu36I 1660 CCTCAGG CC^T[ACGT]AGG
4259 CCTCAGG
CfoI 19 GCGC GCG^C
59 GCGC
DraIII 3489 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
4286 CACAAGGTG
DrdI 378 GACAGTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4247 GACAGCGGTGTC
DsaI 22 CCGCGG C^C[GA][CT]GG
4318 CCATGG
Eco81I 1660 CCTCAGG CC^T[ACGT]AGG
4259 CCTCAGG
EcoT22I 1228 ATGCAT ATGCA^T
1337 ATGCAT
FauI 1494 CCCGC CCCGC(4/6)
2920 GCGGG
FbaI 1972 TGATCA T^GATCA
4882 TGATCA
HaeII 18 GGCGCC [GA]GCGC^[CT]
58 AGCGCC
HhaI 19 GCGC GCG^C
59 GCGC
HinP1I 19 GCGC G^CGC
59 GCGC
MflI 1257 AGATCT [GA]^GATC[CT]
4000 GGATCT
MluNI 2726 TGGCCA TGG^CCA
4650 TGGCCA
MscI 2726 TGGCCA TGG^CCA
4650 TGGCCA
NdeI 2681 CATATG CA^TATG
3622 CATATG
NsiI 1228 ATGCAT ATGCA^T
1337 ATGCAT
SpeI 788 ACTAGT A^CTAGT
1892 ACTAGT
SspBI 1037 TGTACA T^GTACA
3637 TGTACA
StuI 584 AGGCCT AGG^CCT
3611 AGGCCT
XcmI 361 CCAGACCCTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1885 CCAACTCACTAGTGG
XhoII 1257 AGATCT [GA]^GATC[CT]
4000 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspHI BspMI BsrDI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
Cac8I Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoO65I
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XmnI