################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AccIII AflII AgeI AocI Aor51HI AscI AseI AsnI AvrII BbeI BbvCI BciVI BfrI BlnI BlpI Bpu1102I BsaWI BseAI BsiEI BsiWI Bsp1407I BspEI BsrBI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstZ17I Bsu36I CelII CpoI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I FseI HgaI KasI KspI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NruI PacI PinAI PmeI PshBI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI Sse8387I SspBI SspI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 1277 GACGTC GACGT^C Acc65I 467 GGTACC G^GTACC AclI 861 AACGTT AA^CGTT AcyI 1277 GACGTC G[GA]^CG[CT]C AhdI 4283 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 422 GGGCCC GGGCC^C Asp700I 158 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 467 GGTACC G^GTACC AspEI 4283 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1447 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 495 TGCGCA TGC^GCA BbrPI 3835 CACGTG CAC^GTG BclI 687 TGATCA T^GATCA BglI 1205 GCCGTCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1378 AGATCT A^GATCT BsaHI 1277 GACGTC G[GA]^CG[CT]C BsmBI 331 CGTCTC CGTCTC(1/5) BspDI 6868 ATCGAT AT^CGAT BsrFI 2385 ACCGGC [GA]^CCGG[CT] BstBI 4436 TTCGAA TT^CGAA Cfr10I 2385 ACCGGC [GA]^CCGG[CT] ClaI 6868 ATCGAT AT^CGAT Eam1105I 4283 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 4018 GATATC GAT^ATC FbaI 687 TGATCA T^GATCA FspI 495 TGCGCA TGC^GCA HaeII 1698 GGCGCT [GA]GCGC^[CT] Hin1I 1277 GACGTC G[GA]^CG[CT]C HindIII 6056 AAGCTT A^AGCTT KpnI 467 GGTACC GGTAC^C NdeI 4147 CATATG CA^TATG NspV 4436 TTCGAA TT^CGAA PaeR7I 5813 CTCGAG C^TCGAG PflFI 1447 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 3835 CACGTG CAC^GTG PmlI 3835 CACGTG CAC^GTG PshAI 2895 GACTCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 861 AACGTT AA^CGTT SapI 629 GAAGAGC GCTCTTC(1/4) SfuI 4436 TTCGAA TT^CGAA SphI 3241 GCATGC GCATG^C SwaI 6071 ATTTAAAT ATTT^AAAT Tth111I 1447 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 5241 TCTAGA T^CTAGA XhoI 5813 CTCGAG C^TCGAG XmnI 158 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 785 GTCTAC GT^[AC][GT]AC 4297 GTAGAC AccII 374 CGCG CG^CG 2369 CGCG Alw44I 1033 GTGCAC G^TGCAC 5945 GTGCAC ApaLI 1033 GTGCAC G^TGCAC 5945 GTGCAC BamHI 1023 GGATCC G^GATCC 5480 GGATCC BbsI 805 GTCTTC GAAGAC(2/6) 2286 GTCTTC BcgI 701 GCACGGCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 6599 GCAAAGTTCTCG BpuAI 805 GTCTTC GAAGAC(2/6) 2286 GTCTTC BsaAI 788 TACGTG [CT]AC^GT[GA] 3835 CACGTG BsaBI 2774 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4207 GATTGAAATC BsaI 6250 GGTCTC GGTCTC(1/5) 6615 GGTCTC BstAPI 2389 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2451 GCAAAATCTGC BstUI 374 CGCG CG^CG 2369 CGCG DraIII 1740 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG 4094 CACCCTGTG EcoT22I 1601 ATGCAT ATGCA^T 4073 ATGCAT FauI 1900 CCCGC CCCGC(4/6) 2367 CCCGC HpaI 3137 GTTAAC GTT^AAC 5380 GTTAAC MamI 2774 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4207 GATTGAAATC MvnI 374 CGCG CG^CG 2369 CGCG NsiI 1601 ATGCAT ATGCA^T 4073 ATGCAT ScaI 4482 AGTACT AGT^ACT 5817 AGTACT SpeI 3521 ACTAGT A^CTAGT 4966 ACTAGT XcmI 1258 CCAGAGTGACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4960 CCAGTCACTAGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspLU11I BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DrdI DsaI EaeI EarI EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII