##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AccII AccIII AcyI AflII
Aor51HI ApaI AscI AspI AviII
AvrII BamHI BbeI BbrPI BcgI
BcnI BfrI BglI BlnI BsaAI
BsaHI BseAI BsiEI BsiWI BspDI
BspEI BspLU11I BsrBI BssHII BstEII
BstPI BstUI ClaI CpoI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
FauI FseI FspI HaeII HgaI
Hin1I KasI KspI MfeI MluI
MroI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NotI NruI
PacI PflFI PmaCI PmeI PmlI
PvuI RsrII SacII SapI SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI Sse8387I SwaI Tth111I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AgeI 750 ACCGGT A^CCGGT
AhdI 5375 GACTTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2828 CCTCAGG CC^T[ACGT]AGG
AseI 847 ATTAAT AT^TAAT
AsnI 847 ATTAAT AT^TAAT
AspEI 5375 GACTTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 2202 CCTCAGC CCTCAGC(-5/-2)
BsaI 1882 GAGACC GGTCTC(1/5)
Bsp1407I 5502 TGTACA T^GTACA
BspMI 2839 ACCTGC ACCTGC(4/8)
BsrGI 5502 TGTACA T^GTACA
BstBI 1201 TTCGAA TT^CGAA
Bsu36I 2828 CCTCAGG CC^T[ACGT]AGG
CfoI 4825 GCGC GCG^C
DrdI 1295 GACTGGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 5375 GACTTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2828 CCTCAGG CC^T[ACGT]AGG
HhaI 4825 GCGC GCG^C
HinP1I 4825 GCGC G^CGC
NspV 1201 TTCGAA TT^CGAA
PaeR7I 3020 CTCGAG C^TCGAG
PinAI 750 ACCGGT A^CCGGT
PshBI 847 ATTAAT AT^TAAT
SalI 3865 GTCGAC G^TCGAC
ScaI 3400 AGTACT AGT^ACT
SfuI 1201 TTCGAA TT^CGAA
SspBI 5502 TGTACA T^GTACA
XhoI 3020 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 2041 GGTACC G^GTACC
3012 GGTACC
AclI 2297 AACGTT AA^CGTT
3300 AACGTT
Alw44I 3033 GTGCAC G^TGCAC
4507 GTGCAC
ApaLI 3033 GTGCAC G^TGCAC
4507 GTGCAC
Asp700I 406 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1960 GAATATATTC
Asp718I 2041 GGTACC G^GTACC
3012 GGTACC
BalI 2712 TGGCCA TGG^CCA
2944 TGGCCA
BanII 5125 GAGCTC G[GA]GC[CT]^C
5457 GAGCTC
BciVI 3731 GGATAC GTATCC(6/5)
4915 GGATAC
BclI 56 TGATCA T^GATCA
1328 TGATCA
BglII 434 AGATCT A^GATCT
5227 AGATCT
BlpI 907 GCTGAGC GC^T[ACGT]AGC
1516 GCTGAGC
BpmI 772 CTGGAG CTGGAG(16/14)
1687 CTGGAG
Bpu1102I 907 GCTGAGC GC^T[ACGT]AGC
1516 GCTGAGC
BsaWI 750 ACCGGT [AT]^CCGG[AT]
4844 TCCGGT
BseRI 976 CTCCTC GAGGAG(10/8)
4494 GAGGAG
BsgI 2972 CTGCAC GTGCAG(16/14)
2990 CTGCAC
BsmBI 157 GAGACG CGTCTC(1/5)
4573 GAGACG
BsrFI 48 ACCGGC [GA]^CCGG[CT]
750 ACCGGT
BssSI 1055 CTCGTG CACGAG(-5/-1)
5522 CTCGTG
Bst1107I 2366 GTATAC GTA^TAC
3615 GTATAC
BstZ17I 2366 GTATAC GTA^TAC
3615 GTATAC
CelII 907 GCTGAGC GC^T[ACGT]AGC
1516 GCTGAGC
Cfr10I 48 ACCGGC [GA]^CCGG[CT]
750 ACCGGT
DraIII 958 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
3171 CACATAGTG
DsaI 390 CCATGG C^C[GA][CT]GG
2137 CCATGG
EarI 229 CTCTTC CTCTTC(1/4)
4489 GAAGAG
EcoNI 2844 CCTCTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4385 CCTTCTTCAGG
EcoRV 1498 GATATC GAT^ATC
3663 GATATC
FbaI 56 TGATCA T^GATCA
1328 TGATCA
HpaI 2666 GTTAAC GTT^AAC
4566 GTTAAC
KpnI 2041 GGTACC GGTAC^C
3012 GGTACC
Ksp632I 229 CTCTTC CTCTTC(1/4)
4489 GAAGAG
MluNI 2712 TGGCCA TGG^CCA
2944 TGGCCA
MscI 2712 TGGCCA TGG^CCA
2944 TGGCCA
MslI 589 CACTACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2326 CACAGAAATG
NcoI 390 CCATGG C^CATGG
2137 CCATGG
NheI 928 GCTAGC G^CTAGC
4020 GCTAGC
PshAI 491 GACTTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4400 GACTGTGGTC
Psp1406I 2297 AACGTT AA^CGTT
3300 AACGTT
SacI 5125 GAGCTC GAGCT^C
5457 GAGCTC
SpeI 3781 ACTAGT A^CTAGT
4543 ACTAGT
StuI 1691 AGGCCT AGG^CCT
2086 AGGCCT
XcmI 953 CCACACACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1109 CCATACAGCTGCTGG
XmnI 406 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1960 GAATATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BbsI BbvI
BfaI BmyI BpuAI BsaBI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspHI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII EaeI EcoO109I
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HincII HindII HindIII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XhoII