##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AccIII AclI AgeI AhdI Aor51HI
AscI AspEI AspI AviII BbeI
BcgI BsaBI BseAI BsiWI BsmBI
BspDI BspEI BsrBI BssHII BssSI
BstAPI ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FseI FspI HaeII
HgaI KasI KspI MamI MluI
MroI NarI NheI NotI NruI
NsiI PacI PflFI PinAI PmeI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfoI SgrAI
SnaBI SphI Sse8387I SwaI Tth111I
XbaI XcmI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AatII 3342 GACGTC GACGT^C
Acc65I 684 GGTACC G^GTACC
AccII 1549 CGCG CG^CG
AcyI 3342 GACGTC G[GA]^CG[CT]C
AflII 1203 CTTAAG C^TTAAG
AflIII 3891 ACATGT A^C[GA][CT]GT
Alw44I 4808 GTGCAC G^TGCAC
AocI 829 CCTCAGG CC^T[ACGT]AGG
ApaLI 4808 GTGCAC G^TGCAC
AseI 2678 ATTAAT AT^TAAT
AsnI 2678 ATTAAT AT^TAAT
Asp700I 1671 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 684 GGTACC G^GTACC
AvrII 3916 CCTAGG C^CTAGG
BalI 1644 TGGCCA TGG^CCA
BbrPI 2768 CACGTG CAC^GTG
BciVI 937 GTATCC GTATCC(6/5)
BfrI 1203 CTTAAG C^TTAAG
BglI 2327 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1673 AGATCT A^GATCT
BlnI 3916 CCTAGG C^CTAGG
BsaHI 3342 GACGTC G[GA]^CG[CT]C
BsiEI 3191 CGGTCG CG[GA][CT]^CG
BspLU11I 3891 ACATGT A^CATGT
BspMI 4804 GCAGGT ACCTGC(4/8)
BsrFI 3968 GCCGGC [GA]^CCGG[CT]
Bst1107I 4544 GTATAC GTA^TAC
BstBI 1433 TTCGAA TT^CGAA
BstUI 1549 CGCG CG^CG
BstZ17I 4544 GTATAC GTA^TAC
Bsu36I 829 CCTCAGG CC^T[ACGT]AGG
CfoI 1550 GCGC GCG^C
Cfr10I 3968 GCCGGC [GA]^CCGG[CT]
Eco81I 829 CCTCAGG CC^T[ACGT]AGG
HhaI 1550 GCGC GCG^C
Hin1I 3342 GACGTC G[GA]^CG[CT]C
HinP1I 1550 GCGC G^CGC
HindIII 4814 AAGCTT A^AGCTT
KpnI 684 GGTACC GGTAC^C
MluNI 1644 TGGCCA TGG^CCA
MscI 1644 TGGCCA TGG^CCA
MvnI 1549 CGCG CG^CG
NaeI 3968 GCCGGC GCC^GGC
NgoMIV 3968 GCCGGC G^CCGGC
NspV 1433 TTCGAA TT^CGAA
PaeR7I 2492 CTCGAG C^TCGAG
PmaCI 2768 CACGTG CAC^GTG
PmlI 2768 CACGTG CAC^GTG
PshBI 2678 ATTAAT AT^TAAT
SacI 2503 GAGCTC GAGCT^C
SapI 2059 GAAGAGC GCTCTTC(1/4)
SexAI 205 ACCTGGT A^CC[AT]GGT
SfuI 1433 TTCGAA TT^CGAA
SmaI 2330 CCCGGG CCC^GGG
SpeI 972 ACTAGT A^CTAGT
SspI 4391 AATATT AAT^ATT
XhoI 2492 CTCGAG C^TCGAG
XmaCI 2330 CCCGGG C^CCGGG
XmaI 2330 CCCGGG C^CCGGG
XmnI 1671 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
ApaI 996 GGGCCC GGGCC^C
3717 GGGCCC
BanI 684 GGTACC G^G[CT][GA]CC
5109 GGCACC
BclI 321 TGATCA T^GATCA
2012 TGATCA
BlpI 3309 GCTGAGC GC^T[ACGT]AGC
5559 GCTGAGC
Bpu1102I 3309 GCTGAGC GC^T[ACGT]AGC
5559 GCTGAGC
BsaAI 2602 TACGTG [CT]AC^GT[GA]
2768 CACGTG
BsaI 901 GGTCTC GGTCTC(1/5)
3394 GGTCTC
BsaWI 2587 TCCGGT [AT]^CCGG[AT]
3674 ACCGGA
BseRI 1208 GAGGAG GAGGAG(10/8)
1472 GAGGAG
Bsp1407I 4969 TGTACA T^GTACA
5602 TGTACA
BsrGI 4969 TGTACA T^GTACA
5602 TGTACA
BstEII 2440 GGTGACC G^GT[ACGT]ACC
4563 GGTCACC
BstPI 2440 GGTGACC G^GT[ACGT]ACC
4563 GGTCACC
CelII 3309 GCTGAGC GC^T[ACGT]AGC
5559 GCTGAGC
EcoNI 1585 CCTGTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2470 CCTACACAAGG
EcoO65I 2440 GGTGACC G^GT[ACGT]ACC
4563 GGTCACC
FbaI 321 TGATCA T^GATCA
2012 TGATCA
NdeI 4074 CATATG CA^TATG
5481 CATATG
PstI 1958 CTGCAG CTGCA^G
2113 CTGCAG
ScaI 4494 AGTACT AGT^ACT
4879 AGTACT
SspBI 4969 TGTACA T^GTACA
5602 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BamHI BanII BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspHI BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MnlI MseI MslI
MspA1I MspI MunI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII