##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvrII       
BamHI       BbeI        BbrPI       BciVI       BclI        
BfrI        BglII       BlnI        BsaAI       BsaBI       
BsaHI       BseAI       BsiEI       BsiWI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrGI       BssHII      
BstBI       ClaI        CpoI        DraI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HaeII       
HgaI        Hin1I       HindIII     HpaI        KasI        
KpnI        KspI        MamI        MluI        MroI        
NarI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        ScaI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SspBI       
SspI        SwaI        Tth111I     Van91I      XbaI        
XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AccII        1017  CGCG                 CG^CG
AcsI         3061  GAATTT               [GA]^AATT[CT]
ApoI         3061  GAATTT               [GA]^AATT[CT]
AviII         470  TGCGCA               TGC^GCA
BsaWI         166  ACCGGA               [AT]^CCGG[AT]
BsmBI        2921  GAGACG               CGTCTC(1/5)
BsmI         3030  GAATGC               GAATGC(1/-1)
BspHI        1163  TCATGA               T^CATGA
Bst1107I      317  GTATAC               GTA^TAC
BstEII       2146  GGTCACC              G^GT[ACGT]ACC
BstPI        2146  GGTCACC              G^GT[ACGT]ACC
BstUI        1017  CGCG                 CG^CG
BstZ17I       317  GTATAC               GTA^TAC
CfoI          471  GCGC                 GCG^C
DraIII       1937  CACGTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1951  GACCGCTGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      2146  GGTCACC              G^GT[ACGT]ACC
FspI          470  TGCGCA               TGC^GCA
HhaI          471  GCGC                 GCG^C
HinP1I        471  GCGC                 G^CGC
MfeI         2899  CAATTG               C^AATTG
MunI         2899  CAATTG               C^AATTG
MvnI         1017  CGCG                 CG^CG
NaeI         1575  GCCGGC               GCC^GGC
NgoMIV       1575  GCCGGC               G^CCGGC
RcaI         1163  TCATGA               T^CATGA
SacI         2293  GAGCTC               GAGCT^C
SexAI        1975  ACCTGGT              A^CC[AT]GGT
SfiI         2565  GGCCCAGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccI          317  GTATAC               GT^[AC][GT]AC
             3037  GTCTAC              
AocI         2059  CCTGAGG              CC^T[ACGT]AGG
             2191  CCTGAGG             
BalI           90  TGGCCA               TGG^CCA
             1046  TGGCCA              
BsaI          364  GAGACC               GGTCTC(1/5)
             1464  GGTCTC              
BsrFI        1394  ACCGGC               [GA]^CCGG[CT]
             1575  GCCGGC              
BssSI         568  CACGAG               CACGAG(-5/-1)
             1694  CACGAG              
BstAPI       1113  GCACTGTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2788  GCAGGACCTGC         
BstXI        2163  CCAGCTCAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2179  CCAGGATCATGG        
BstYI         573  GGATCT               [GA]^GATC[CT]
             3139  GGATCT              
Bsu36I       2059  CCTGAGG              CC^T[ACGT]AGG
             2191  CCTGAGG             
Cfr10I       1394  ACCGGC               [GA]^CCGG[CT]
             1575  GCCGGC              
Eco81I       2059  CCTGAGG              CC^T[ACGT]AGG
             2191  CCTGAGG             
MflI          573  GGATCT               [GA]^GATC[CT]
             3139  GGATCT              
MluNI          90  TGGCCA               TGG^CCA
             1046  TGGCCA              
MscI           90  TGGCCA               TGG^CCA
             1046  TGGCCA              
MseI         3180  TTAA                 T^TAA
             3279  TTAA                
PaeR7I        844  CTCGAG               C^TCGAG
             2509  CTCGAG              
SphI         1680  GCATGC               GCATG^C
             2862  GCATGC              
StuI          230  AGGCCT               AGG^CCT
             2381  AGGCCT              
Tru9I        3180  TTAA                 T^TAA
             3279  TTAA                
XcmI         1099  CCAGGGGATGATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2466  CCAGCCCTTCACTGG     
XhoI          844  CTCGAG               C^TCGAG
             2509  CTCGAG              
XhoII         573  GGATCT               [GA]^GATC[CT]
             3139  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (  105 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
ApaI        AspHI       AvaI        AvaII       BanI        
BanII       BbsI        BbvCI       BbvI        BcgI        
BcnI        BfaI        BglI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrDI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       CelII       Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MnlI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PpuMI       PstI        
PvuII       RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        SmlI        
StyI        TaqI        TfiI        TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XmaCI       XmaI