##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccIII AcyI AflII AgeI
Alw44I Aor51HI ApaLI AscI AspI
AviII AvrII BamHI BbeI BfrI
BglI BlnI BsaBI BsaHI BseAI
BsiWI BsmBI BspDI BspEI BspHI
BssHII BstBI BstEII BstPI ClaI
CpoI DraIII DrdI EagI EclXI
Eco47III Eco52I EcoO65I EcoRI FseI
FspI HaeII Hin1I HpaI KasI
KspI MamI MluI MroI NarI
NheI NotI NruI NspV PacI
PflFI PflMI PinAI PmeI PvuI
RcaI RsrII SacII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SspI Tth111I Van91I
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 5217 GTATAC GT^[AC][GT]AC
AccII 4559 CGCG CG^CG
AclI 3597 AACGTT AA^CGTT
AhdI 707 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 5591 GGATC GGATC(4/5)
AseI 5517 ATTAAT AT^TAAT
AsnI 5517 ATTAAT AT^TAAT
AspEI 707 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 2962 CACGTG CAC^GTG
BcgI 1553 GCATCAAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1710 AGATCT A^GATCT
BsaAI 2962 CACGTG [CT]AC^GT[GA]
BsiEI 2446 CGACCG CG[GA][CT]^CG
BsmI 4554 GCATTC GAATGC(1/-1)
BsrBI 1608 CCGCTC CCGCTC(-3/-3)
Bst1107I 5217 GTATAC GTA^TAC
BstUI 4559 CGCG CG^CG
BstZ17I 5217 GTATAC GTA^TAC
CfoI 4560 GCGC GCG^C
DraI 5455 TTTAAA TTT^AAA
Eam1105I 707 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HhaI 4560 GCGC GCG^C
HinP1I 4560 GCGC G^CGC
MvnI 4559 CGCG CG^CG
NdeI 4436 CATATG CA^TATG
PmaCI 2962 CACGTG CAC^GTG
PmlI 2962 CACGTG CAC^GTG
PshAI 1679 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5517 ATTAAT AT^TAAT
Psp1406I 3597 AACGTT AA^CGTT
SacI 3416 GAGCTC GAGCT^C
SbfI 2711 CCTGCAGG CCTGCA^GG
SmaI 1390 CCCGGG CCC^GGG
SpeI 4253 ACTAGT A^CTAGT
SphI 4941 GCATGC GCATG^C
Sse8387I 2711 CCTGCAGG CCTGCA^GG
SwaI 5454 ATTTAAAT ATTT^AAAT
XmaCI 1390 CCCGGG C^CCGGG
XmaI 1390 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AocI 2763 CCTCAGG CC^T[ACGT]AGG
3888 CCTGAGG
Asp700I 222 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
675 GAAGCATTTC
AspHI 3059 GAGCAC G[AT]GC[AT]^C
3416 GAGCTC
BalI 4419 TGGCCA TGG^CCA
5712 TGGCCA
BclI 1166 TGATCA T^GATCA
4370 TGATCA
BsaWI 3028 ACCGGA [AT]^CCGG[AT]
5297 ACCGGA
BsiHKAI 3059 GAGCAC G[AT]GC[AT]^C
3416 GAGCTC
Bsp1407I 3317 TGTACA T^GTACA
5608 TGTACA
BspLU11I 734 ACATGT A^CATGT
4177 ACATGT
BsrFI 2870 GCCGGC [GA]^CCGG[CT]
4858 GCCGGC
BsrGI 3317 TGTACA T^GTACA
5608 TGTACA
BssSI 4697 CACGAG CACGAG(-5/-1)
3920 CTCGTG
BstYI 1710 AGATCT [GA]^GATC[CT]
5591 GGATCT
Bsu36I 2763 CCTCAGG CC^T[ACGT]AGG
3888 CCTGAGG
Cfr10I 2870 GCCGGC [GA]^CCGG[CT]
4858 GCCGGC
Eco81I 2763 CCTCAGG CC^T[ACGT]AGG
3888 CCTGAGG
EcoRV 1094 GATATC GAT^ATC
1380 GATATC
FbaI 1166 TGATCA T^GATCA
4370 TGATCA
MfeI 4723 CAATTG C^AATTG
5708 CAATTG
MflI 1710 AGATCT [GA]^GATC[CT]
5591 GGATCT
MluNI 4419 TGGCCA TGG^CCA
5712 TGGCCA
MscI 4419 TGGCCA TGG^CCA
5712 TGGCCA
MunI 4723 CAATTG C^AATTG
5708 CAATTG
NaeI 2870 GCCGGC GCC^GGC
4858 GCCGGC
NgoMIV 2870 GCCGGC G^CCGGC
4858 GCCGGC
PaeR7I 1318 CTCGAG C^TCGAG
1561 CTCGAG
PpuMI 1368 AGGACCC [GA]G^G[AT]CC[CT]
4919 AGGTCCT
SapI 540 GAAGAGC GCTCTTC(1/4)
3207 GCTCTTC
SspBI 3317 TGTACA T^GTACA
5608 TGTACA
XbaI 972 TCTAGA T^CTAGA
3113 TCTAGA
XhoI 1318 CTCGAG C^TCGAG
1561 CTCGAG
XhoII 1710 AGATCT [GA]^GATC[CT]
5591 GGATCT
XmnI 222 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
675 GAAGCATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AflIII
AluI AlwNI ApaI ApoI Asp718I
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BciVI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstXI Cac8I CelII
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeIII HapII HgaI HincII HindII
HindIII HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NsiI
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI