##################################
# NO CUT ENZYMES (   62 enzymes) #
##################################

AatII       Acc65I      AccIII      AgeI        Alw44I      
ApaLI       AscI        AseI        AsnI        Asp718I     
AspI        AviII       BamHI       BsaBI       BseAI       
BsiWI       Bsp1407I    BspDI       BspEI       BspLU11I    
BsrGI       BssHII      BssSI       Bst1107I    BstZ17I     
ClaI        DraIII      DrdI        EcoRV       EcoT22I     
FseI        FspI        HpaI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MunI        
NheI        NotI        NruI        NsiI        PacI        
PflFI       PinAI       PmeI        PshBI       PvuI        
SacII       SbfI        ScaI        SexAI       SfiI        
SgrAI       SpeI        SphI        Sse8387I    SspBI       
SwaI        Tth111I     


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccI          289  GTCGAC               GT^[AC][GT]AC
AclI         5452  AACGTT               AA^CGTT
AflII        3280  CTTAAG               C^TTAAG
AhdI         1579  GACCATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI         3105  GGATC                GGATC(4/5)
AocI         1997  CCTGAGG              CC^T[ACGT]AGG
AspEI        1579  GACCATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BclI         4727  TGATCA               T^GATCA
BfrI         3280  CTTAAG               C^TTAAG
BglII        4321  AGATCT               A^GATCT
BlpI         2254  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2254  GCTCAGC              GC^T[ACGT]AGC
BsaWI         657  ACCGGA               [AT]^CCGG[AT]
BspHI        4223  TCATGA               T^CATGA
BstBI         849  TTCGAA               TT^CGAA
BstEII       1669  GGTCACC              G^GT[ACGT]ACC
BstPI        1669  GGTCACC              G^GT[ACGT]ACC
Bsu36I       1997  CCTGAGG              CC^T[ACGT]AGG
CelII        2254  GCTCAGC              GC^T[ACGT]AGC
CpoI         1164  CGGACCG              CG^G[AT]CCG
DraI         5465  TTTAAA               TTT^AAA
Eam1105I     1579  GACCATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1997  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      1669  GGTCACC              G^GT[ACGT]ACC
EcoRI        4586  GAATTC               G^AATTC
FbaI         4727  TGATCA               T^GATCA
NdeI          870  CATATG               CA^TATG
NspV          849  TTCGAA               TT^CGAA
PaeR7I       1177  CTCGAG               C^TCGAG
Psp1406I     5452  AACGTT               AA^CGTT
RcaI         4223  TCATGA               T^CATGA
RsrII        1164  CGGACCG              CG^G[AT]CCG
SalI          289  GTCGAC               G^TCGAC
SfuI          849  TTCGAA               TT^CGAA
SmaI         2096  CCCGGG               CCC^GGG
SnaBI        4804  TACGTA               TAC^GTA
XbaI         3024  TCTAGA               T^CTAGA
XhoI         1177  CTCGAG               C^TCGAG
XmaCI        2096  CCCGGG               C^CCGGG
XmaI         2096  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AcsI         3605  AAATTT               [GA]^AATT[CT]
             4586  GAATTC              
AflIII        726  ACGTGT               A^C[GA][CT]GT
             2363  ACGTGT              
ApoI         3605  AAATTT               [GA]^AATT[CT]
             4586  GAATTC              
Asp700I       697  GAAGGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3291  GAACAGTTTC          
AvrII        1856  CCTAGG               C^CTAGG
             4168  CCTAGG              
BbeI          386  GGCGCC               GGCGC^C
             1784  GGCGCC              
BbrPI        1079  CACGTG               CAC^GTG
             4257  CACGTG              
BcgI          383  CGAGGCGCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              841  GCAGCAGGTTCG        
BciVI        1864  GGATAC               GTATCC(6/5)
             4308  GGATAC              
BlnI         1856  CCTAGG               C^CTAGG
             4168  CCTAGG              
BsaI         2971  GGTCTC               GGTCTC(1/5)
             4423  GGTCTC              
BsmI           10  GAATGC               GAATGC(1/-1)
             4050  GCATTC              
BsrBI        1648  CCGCTC               CCGCTC(-3/-3)
             2110  CCGCTC              
BsrDI        4266  CATTGC               GCAATG(2/0)
             1931  GCAATG              
BstAPI       1699  GCACAGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2346  GCAGGCTTTGC         
BstYI        3105  GGATCT               [GA]^GATC[CT]
             4321  AGATCT              
HincII        289  GTCGAC               GT[CT]^[GA]AC
             1576  GTTGAC              
HindII        289  GTCGAC               GT[CT]^[GA]AC
             1576  GTTGAC              
HindIII      3619  AAGCTT               A^AGCTT
             3839  AAGCTT              
KasI          386  GGCGCC               G^GCGCC
             1784  GGCGCC              
MflI         3105  GGATCT               [GA]^GATC[CT]
             4321  AGATCT              
NarI          386  GGCGCC               GG^CGCC
             1784  GGCGCC              
NspI          999  GCATGT               [GA]CATG^[CT]
             3704  ACATGC              
PflMI        1224  CCATTTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3781  CCAGCCCTTGG         
PmaCI        1079  CACGTG               CAC^GTG
             4257  CACGTG              
PmlI         1079  CACGTG               CAC^GTG
             4257  CACGTG              
PshAI          19  GACTTGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3591  GACCCAAGTC          
PstI         1711  CTGCAG               CTGCA^G
             4136  CTGCAG              
SapI         2505  GCTCTTC              GCTCTTC(1/4)
             3530  GCTCTTC             
SfoI          386  GGCGCC               GGC^GCC
             1784  GGCGCC              
Van91I       1224  CCATTTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3781  CCAGCCCTTGG         
XcmI          672  CCAGCTACCAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1960  CCAGCCCCTCACTGG     
XhoII        3105  GGATCT               [GA]^GATC[CT]
             4321  AGATCT              
XmnI          697  GAAGGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3291  GAACAGTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  122 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       Aor51HI     ApaI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbsI        
BbvCI       BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BpuAI       BsaAI       BsaHI       
BsaJI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EagI        
EarI        EclXI       Eco47III    Eco52I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NcoI        NdeII       NgoMIV      NlaIII      NlaIV       
PleI        PpuMI       PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I