##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Aor51HI AscI
Asp718I AspEI AspI AvaI AviII
BalI BanI BbeI BbrPI BcgI
BciVI BcnI BglI BlpI Bpu1102I
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI BspEI
BspHI BsrBI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII CpoI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV FauI FseI
FspI HaeII HgaI Hin1I HincII
HindII HpaI KasI KpnI KspI
MluI MluNI MroI MscI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Alw44I 3752 GTGCAC G^TGCAC
AlwNI 1761 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 208 GGGCCC GGGCC^C
ApaLI 3752 GTGCAC G^TGCAC
AspHI 3752 GTGCAC G[AT]GC[AT]^C
AvrII 79 CCTAGG C^CTAGG
BanII 208 GGGCCC G[GA]GC[CT]^C
BbvCI 1454 CCTCAGC CCTCAGC(-5/-2)
BlnI 79 CCTAGG C^CTAGG
BsiHKAI 3752 GTGCAC G[AT]GC[AT]^C
BspDI 3197 ATCGAT AT^CGAT
BspLU11I 3698 ACATGT A^CATGT
BsrFI 2194 GCCGGT [GA]^CCGG[CT]
CfoI 941 GCGC GCG^C
Cfr10I 2194 GCCGGT [GA]^CCGG[CT]
ClaI 3197 ATCGAT AT^CGAT
DsaI 582 CCATGG C^C[GA][CT]GG
EcoRI 3737 GAATTC G^AATTC
EcoT22I 1922 ATGCAT ATGCA^T
HapII 2195 CCGG C^CGG
HhaI 941 GCGC GCG^C
HinP1I 941 GCGC G^CGC
HpaII 2195 CCGG C^CGG
MfeI 5446 CAATTG C^AATTG
MspA1I 3314 CAGCTG C[AC]G^C[GT]G
MspI 2195 CCGG C^CGG
MunI 5446 CAATTG C^AATTG
NcoI 582 CCATGG C^CATGG
NsiI 1922 ATGCAT ATGCA^T
NspI 3698 ACATGT [GA]CATG^[CT]
PflMI 131 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3076 GACTAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 3314 CAGCTG CAG^CTG
SapI 3425 GAAGAGC GCTCTTC(1/4)
Van91I 131 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1690 TCTAGA T^CTAGA
XcmI 4078 CCACTAGACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AflIII 3698 ACATGT A^C[GA][CT]GT
4822 ACACGT
AocI 737 CCTAAGG CC^T[ACGT]AGG
4381 CCTAAGG
AseI 3833 ATTAAT AT^TAAT
4674 ATTAAT
AsnI 3833 ATTAAT AT^TAAT
4674 ATTAAT
BclI 2515 TGATCA T^GATCA
3481 TGATCA
BsaI 2197 GGTCTC GGTCTC(1/5)
5417 GGTCTC
BspMI 5374 ACCTGC ACCTGC(4/8)
2231 GCAGGT
BstAPI 1924 GCATATAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3872 GCAGCATTTGC
Bsu36I 737 CCTAAGG CC^T[ACGT]AGG
4381 CCTAAGG
Eco81I 737 CCTAAGG CC^T[ACGT]AGG
4381 CCTAAGG
EcoNI 1490 CCTAAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3769 CCTTGGAGAGG
FbaI 2515 TGATCA T^GATCA
3481 TGATCA
NdeI 1585 CATATG CA^TATG
2376 CATATG
PshBI 3833 ATTAAT AT^TAAT
4674 ATTAAT
TaqI 2948 TCGA T^CGA
3198 TCGA
TthHB8I 2948 TCGA T^CGA
3198 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwI ApoI Asp700I AvaII
BamHI BbsI BbvI BfaI BfrI
BglII BmyI BpmI BpuAI BsaBI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SpeI
SspBI SspI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII XmnI