################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AatII Acc65I AccIII AclI Alw44I Aor51HI ApaLI AscI Asp718I AviII AvrII BbeI BciVI BglII BlnI BsaBI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrBI BssHII BssSI Bst1107I BstEII BstPI BstZ17I ClaI CpoI EagI EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI HpaI KasI KpnI MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PmeI PshAI Psp1406I PvuI RsrII SacI SalI SapI ScaI SfiI SfoI SgrAI SnaBI SpeI SwaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 4633 GTAGAC GT^[AC][GT]AC AcyI 3401 GGCGTC G[GA]^CG[CT]C AflII 512 CTTAAG C^TTAAG AgeI 2310 ACCGGT A^CCGGT ApaI 1795 GGGCCC GGGCC^C AseI 560 ATTAAT AT^TAAT AsnI 560 ATTAAT AT^TAAT Asp700I 3752 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 3580 TGGCCA TGG^CCA BbvCI 3016 GCTGAGG CCTCAGC(-5/-2) BclI 1447 TGATCA T^GATCA BfrI 512 CTTAAG C^TTAAG BglI 1354 GCCTGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 3401 GGCGTC G[GA]^CG[CT]C BsrFI 2310 ACCGGT [GA]^CCGG[CT] BstBI 652 TTCGAA TT^CGAA Cfr10I 2310 ACCGGT [GA]^CCGG[CT] EcoT22I 2293 ATGCAT ATGCA^T FbaI 1447 TGATCA T^GATCA HgaI 3402 GCGTC GACGC(5/10) Hin1I 3401 GGCGTC G[GA]^CG[CT]C HindIII 3961 AAGCTT A^AGCTT KspI 1504 CCGCGG CCGC^GG MluNI 3580 TGGCCA TGG^CCA MscI 3580 TGGCCA TGG^CCA NsiI 2293 ATGCAT ATGCA^T NspV 652 TTCGAA TT^CGAA PaeR7I 1034 CTCGAG C^TCGAG PinAI 2310 ACCGGT A^CCGGT PshBI 560 ATTAAT AT^TAAT SacII 1504 CCGCGG CCGC^GG SbfI 1355 CCTGCAGG CCTGCA^GG SexAI 1664 ACCAGGT A^CC[AT]GGT SfuI 652 TTCGAA TT^CGAA SphI 2352 GCATGC GCATG^C Sse8387I 1355 CCTGCAGG CCTGCA^GG XhoI 1034 CTCGAG C^TCGAG XmnI 3752 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AhdI 2306 GACAACCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4766 GACATGGAGTC AocI 1295 CCTCAGG CC^T[ACGT]AGG 4178 CCTCAGG AspEI 2306 GACAACCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4766 GACATGGAGTC AspHI 3361 GAGCAC G[AT]GC[AT]^C 3928 GAGCAC BbrPI 1861 CACGTG CAC^GTG 1910 CACGTG BpmI 817 CTGGAG CTGGAG(16/14) 991 CTGGAG BsaAI 1861 CACGTG [CT]AC^GT[GA] 1910 CACGTG BsaI 5614 GAGACC GGTCTC(1/5) 4419 GGTCTC BsaWI 204 ACCGGA [AT]^CCGG[AT] 2310 ACCGGT BsiHKAI 3361 GAGCAC G[AT]GC[AT]^C 3928 GAGCAC BspLU11I 3932 ACATGT A^CATGT 4605 ACATGT BstXI 2189 CCACAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2569 CCAACCTTATGG Bsu36I 1295 CCTCAGG CC^T[ACGT]AGG 4178 CCTCAGG Eam1105I 2306 GACAACCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4766 GACATGGAGTC Eco81I 1295 CCTCAGG CC^T[ACGT]AGG 4178 CCTCAGG HincII 2717 GTTGAC GT[CT]^[GA]AC 3527 GTCAAC HindII 2717 GTTGAC GT[CT]^[GA]AC 3527 GTCAAC PflMI 1839 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5671 CCATCTTGTGG PmaCI 1861 CACGTG CAC^GTG 1910 CACGTG PmlI 1861 CACGTG CAC^GTG 1910 CACGTG SmaI 315 CCCGGG CCC^GGG 1095 CCCGGG Van91I 1839 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5671 CCATCTTGTGG XcmI 2219 CCAGAGTCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4138 CCAGCGTTCAGATGG XmaCI 315 CCCGGG C^CCGGG 1095 CCCGGG XmaI 315 CCCGGG C^CCGGG 1095 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspI AvaI AvaII BamHI BanI BanII BbsI BbvI BcgI BcnI BfaI BlpI BmyI Bpu1102I BpuAI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspHI BspMI BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DraIII DrdI DsaI EaeI EarI EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XbaI XhoII