##################################
# NO CUT ENZYMES (   70 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        Alw44I      
Aor51HI     ApaLI       AscI        Asp718I     AviII       
AvrII       BbeI        BciVI       BglII       BlnI        
BsaBI       BseAI       BsiEI       BsiWI       BsmBI       
BspDI       BspEI       BsrBI       BssHII      BssSI       
Bst1107I    BstEII      BstPI       BstZ17I     ClaI        
CpoI        EagI        EclXI       Eco47III    Eco52I      
EcoO65I     EcoRV       FseI        FspI        HpaI        
KasI        KpnI        MamI        MfeI        MluI        
MroI        MunI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        PacI        
PmeI        PshAI       Psp1406I    PvuI        RsrII       
SacI        SalI        SapI        ScaI        SfiI        
SfoI        SgrAI       SnaBI       SpeI        SwaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccI         4633  GTAGAC               GT^[AC][GT]AC
AcyI         3401  GGCGTC               G[GA]^CG[CT]C
AflII         512  CTTAAG               C^TTAAG
AgeI         2310  ACCGGT               A^CCGGT
ApaI         1795  GGGCCC               GGGCC^C
AseI          560  ATTAAT               AT^TAAT
AsnI          560  ATTAAT               AT^TAAT
Asp700I      3752  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI         3580  TGGCCA               TGG^CCA
BbvCI        3016  GCTGAGG              CCTCAGC(-5/-2)
BclI         1447  TGATCA               T^GATCA
BfrI          512  CTTAAG               C^TTAAG
BglI         1354  GCCTGCAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI        3401  GGCGTC               G[GA]^CG[CT]C
BsrFI        2310  ACCGGT               [GA]^CCGG[CT]
BstBI         652  TTCGAA               TT^CGAA
Cfr10I       2310  ACCGGT               [GA]^CCGG[CT]
EcoT22I      2293  ATGCAT               ATGCA^T
FbaI         1447  TGATCA               T^GATCA
HgaI         3402  GCGTC                GACGC(5/10)
Hin1I        3401  GGCGTC               G[GA]^CG[CT]C
HindIII      3961  AAGCTT               A^AGCTT
KspI         1504  CCGCGG               CCGC^GG
MluNI        3580  TGGCCA               TGG^CCA
MscI         3580  TGGCCA               TGG^CCA
NsiI         2293  ATGCAT               ATGCA^T
NspV          652  TTCGAA               TT^CGAA
PaeR7I       1034  CTCGAG               C^TCGAG
PinAI        2310  ACCGGT               A^CCGGT
PshBI         560  ATTAAT               AT^TAAT
SacII        1504  CCGCGG               CCGC^GG
SbfI         1355  CCTGCAGG             CCTGCA^GG
SexAI        1664  ACCAGGT              A^CC[AT]GGT
SfuI          652  TTCGAA               TT^CGAA
SphI         2352  GCATGC               GCATG^C
Sse8387I     1355  CCTGCAGG             CCTGCA^GG
XhoI         1034  CTCGAG               C^TCGAG
XmnI         3752  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AhdI         2306  GACAACCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4766  GACATGGAGTC         
AocI         1295  CCTCAGG              CC^T[ACGT]AGG
             4178  CCTCAGG             
AspEI        2306  GACAACCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4766  GACATGGAGTC         
AspHI        3361  GAGCAC               G[AT]GC[AT]^C
             3928  GAGCAC              
BbrPI        1861  CACGTG               CAC^GTG
             1910  CACGTG              
BpmI          817  CTGGAG               CTGGAG(16/14)
              991  CTGGAG              
BsaAI        1861  CACGTG               [CT]AC^GT[GA]
             1910  CACGTG              
BsaI         5614  GAGACC               GGTCTC(1/5)
             4419  GGTCTC              
BsaWI         204  ACCGGA               [AT]^CCGG[AT]
             2310  ACCGGT              
BsiHKAI      3361  GAGCAC               G[AT]GC[AT]^C
             3928  GAGCAC              
BspLU11I     3932  ACATGT               A^CATGT
             4605  ACATGT              
BstXI        2189  CCACAGAAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2569  CCAACCTTATGG        
Bsu36I       1295  CCTCAGG              CC^T[ACGT]AGG
             4178  CCTCAGG             
Eam1105I     2306  GACAACCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4766  GACATGGAGTC         
Eco81I       1295  CCTCAGG              CC^T[ACGT]AGG
             4178  CCTCAGG             
HincII       2717  GTTGAC               GT[CT]^[GA]AC
             3527  GTCAAC              
HindII       2717  GTTGAC               GT[CT]^[GA]AC
             3527  GTCAAC              
PflMI        1839  CCATTGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5671  CCATCTTGTGG         
PmaCI        1861  CACGTG               CAC^GTG
             1910  CACGTG              
PmlI         1861  CACGTG               CAC^GTG
             1910  CACGTG              
SmaI          315  CCCGGG               CCC^GGG
             1095  CCCGGG              
Van91I       1839  CCATTGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5671  CCATCTTGTGG         
XcmI         2219  CCAGAGTCTGTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4138  CCAGCGTTCAGATGG     
XmaCI         315  CCCGGG               C^CCGGG
             1095  CCCGGG              
XmaI          315  CCCGGG               C^CCGGG
             1095  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  125 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        AspI        
AvaI        AvaII       BamHI       BanI        BanII       
BbsI        BbvI        BcgI        BcnI        BfaI        
BlpI        BmyI        Bpu1102I    BpuAI       BsaJI       
BseRI       BsgI        BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspHI       BspMI       BsrDI       BsrGI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstYI       Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRI       EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PflFI       PleI        PpuMI       
PstI        PvuII       RcaI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspBI       SspI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     XbaI        XhoII