##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcyI        
AflII       AgeI        AhdI        AocI        Aor51HI     
ApaI        AscI        Asp718I     AspEI       AspI        
AviII       AvrII       BanI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BfrI        BlnI        
BsaAI       BsaHI       BseAI       BsiEI       BsiWI       
BspDI       BspEI       BsrBI       BsrFI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DraII       DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO109I    
EcoO65I     EcoRV       FauI        FseI        FspI        
HaeII       HgaI        Hin1I       KasI        KpnI        
KspI        MfeI        MluI        MroI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmlI        
PpuMI       PvuI        RsrII       SacII       SalI        
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SnaBI       SphI        Sse8387I    SwaI        
Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccI         3375  GTAGAC               GT^[AC][GT]AC
AclI         3603  AACGTT               AA^CGTT
Asp700I       626  GAAGACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI         1149  TGGCCA               TGG^CCA
BamHI        1514  GGATCC               G^GATCC
BglI          239  GCCCTCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         5684  GCTAAGC              GC^T[ACGT]AGC
BpmI         5372  CTGGAG               CTGGAG(16/14)
Bpu1102I     5684  GCTAAGC              GC^T[ACGT]AGC
BsaBI        4810  GATTGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          321  GGTCTC               GGTCTC(1/5)
BsaWI        1299  ACCGGA               [AT]^CCGG[AT]
BsmBI        1920  CGTCTC               CGTCTC(1/5)
BsrDI        3202  CATTGC               GCAATG(2/0)
BssSI        3042  CTCGTG               CACGAG(-5/-1)
BstAPI       1597  GCAAATCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII        5684  GCTAAGC              GC^T[ACGT]AGC
DraIII       3736  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         1149  TGGCCA               [CT]^GGCC[GA]
HindIII      4084  AAGCTT               A^AGCTT
HpaI         3456  GTTAAC               GTT^AAC
MamI         4810  GATTGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1149  TGGCCA               TGG^CCA
MscI         1149  TGGCCA               TGG^CCA
NheI         3650  GCTAGC               G^CTAGC
PmeI         2462  GTTTAAAC             GTTT^AAAC
PshAI        2227  GACATGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     3603  AACGTT               AA^CGTT
SacI         2769  GAGCTC               GAGCT^C
SexAI         570  ACCTGGT              A^CC[AT]GGT
SmaI          210  CCCGGG               CCC^GGG
StuI         5368  AGGCCT               AGG^CCT
XbaI         4026  TCTAGA               T^CTAGA
XcmI         2089  CCATCTGCGGATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         210  CCCGGG               C^CCGGG
XmaI          210  CCCGGG               C^CCGGG
XmnI          626  GAAGACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

Alw44I       4179  GTGCAC               G^TGCAC
             4437  GTGCAC              
ApaLI        4179  GTGCAC               G^TGCAC
             4437  GTGCAC              
AseI         3428  ATTAAT               AT^TAAT
             4922  ATTAAT              
AsnI         3428  ATTAAT               AT^TAAT
             4922  ATTAAT              
AvaI           31  CTCGGG               C^[CT]CG[GA]G
              210  CCCGGG              
BglII         673  AGATCT               A^GATCT
             5154  AGATCT              
BsgI         2169  CTGCAC               GTGCAG(16/14)
             2222  GTGCAG              
BsoBI          31  CTCGGG               C^[CT]CG[GA]G
              210  CCCGGG              
BspHI        3249  TCATGA               T^CATGA
             4914  TCATGA              
CfoI           53  GCGC                 GCG^C
              230  GCGC                
EcoNI        2104  CCTCTACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             5371  CCTGGAGAAGG         
EcoRI         910  GAATTC               G^AATTC
             4932  GAATTC              
EcoT14I      1459  CCAAGG               C^C[AT][AT]GG
             5569  CCAAGG              
HhaI           53  GCGC                 GCG^C
              230  GCGC                
HinP1I         53  GCGC                 G^CGC
              230  GCGC                
PshBI        3428  ATTAAT               AT^TAAT
             4922  ATTAAT              
PvuII          90  CAGCTG               CAG^CTG
             2335  CAGCTG              
RcaI         3249  TCATGA               T^CATGA
             4914  TCATGA              
SapI         2278  GAAGAGC              GCTCTTC(1/4)
             2374  GAAGAGC             
SpeI         3565  ACTAGT               A^CTAGT
             3617  ACTAGT              
StyI         1459  CCAAGG               C^C[AT][AT]GG
             5569  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   98 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        AspHI       AvaII       
BanII       BbsI        BbvI        BclI        BcnI        
BfaI        BmyI        BpuAI       BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    Bsp1407I    BspLU11I    BspMI       
BsrGI       BsrI        BssKI       BstF5I      BstNI       
BstXI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraI        EarI        EcoRII      EcoT22I     
FbaI        Fnu4HI      FokI        HaeIII      HapII       
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PflMI       PleI        PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspBI       SspI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XhoII