################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AccII AccIII AcyI AflII AgeI AhdI AocI Aor51HI ApaI AscI Asp718I AspEI AspI AviII AvrII BanI BbeI BbrPI BbvCI BcgI BciVI BfrI BlnI BsaAI BsaHI BseAI BsiEI BsiWI BspDI BspEI BsrBI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRV FauI FseI FspI HaeII HgaI Hin1I KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmlI PpuMI PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 3375 GTAGAC GT^[AC][GT]AC AclI 3603 AACGTT AA^CGTT Asp700I 626 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 1149 TGGCCA TGG^CCA BamHI 1514 GGATCC G^GATCC BglI 239 GCCCTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 5684 GCTAAGC GC^T[ACGT]AGC BpmI 5372 CTGGAG CTGGAG(16/14) Bpu1102I 5684 GCTAAGC GC^T[ACGT]AGC BsaBI 4810 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 321 GGTCTC GGTCTC(1/5) BsaWI 1299 ACCGGA [AT]^CCGG[AT] BsmBI 1920 CGTCTC CGTCTC(1/5) BsrDI 3202 CATTGC GCAATG(2/0) BssSI 3042 CTCGTG CACGAG(-5/-1) BstAPI 1597 GCAAATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 5684 GCTAAGC GC^T[ACGT]AGC DraIII 3736 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 1149 TGGCCA [CT]^GGCC[GA] HindIII 4084 AAGCTT A^AGCTT HpaI 3456 GTTAAC GTT^AAC MamI 4810 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1149 TGGCCA TGG^CCA MscI 1149 TGGCCA TGG^CCA NheI 3650 GCTAGC G^CTAGC PmeI 2462 GTTTAAAC GTTT^AAAC PshAI 2227 GACATGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 3603 AACGTT AA^CGTT SacI 2769 GAGCTC GAGCT^C SexAI 570 ACCTGGT A^CC[AT]GGT SmaI 210 CCCGGG CCC^GGG StuI 5368 AGGCCT AGG^CCT XbaI 4026 TCTAGA T^CTAGA XcmI 2089 CCATCTGCGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 210 CCCGGG C^CCGGG XmaI 210 CCCGGG C^CCGGG XmnI 626 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Alw44I 4179 GTGCAC G^TGCAC 4437 GTGCAC ApaLI 4179 GTGCAC G^TGCAC 4437 GTGCAC AseI 3428 ATTAAT AT^TAAT 4922 ATTAAT AsnI 3428 ATTAAT AT^TAAT 4922 ATTAAT AvaI 31 CTCGGG C^[CT]CG[GA]G 210 CCCGGG BglII 673 AGATCT A^GATCT 5154 AGATCT BsgI 2169 CTGCAC GTGCAG(16/14) 2222 GTGCAG BsoBI 31 CTCGGG C^[CT]CG[GA]G 210 CCCGGG BspHI 3249 TCATGA T^CATGA 4914 TCATGA CfoI 53 GCGC GCG^C 230 GCGC EcoNI 2104 CCTCTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5371 CCTGGAGAAGG EcoRI 910 GAATTC G^AATTC 4932 GAATTC EcoT14I 1459 CCAAGG C^C[AT][AT]GG 5569 CCAAGG HhaI 53 GCGC GCG^C 230 GCGC HinP1I 53 GCGC G^CGC 230 GCGC PshBI 3428 ATTAAT AT^TAAT 4922 ATTAAT PvuII 90 CAGCTG CAG^CTG 2335 CAGCTG RcaI 3249 TCATGA T^CATGA 4914 TCATGA SapI 2278 GAAGAGC GCTCTTC(1/4) 2374 GAAGAGC SpeI 3565 ACTAGT A^CTAGT 3617 ACTAGT StyI 1459 CCAAGG C^C[AT][AT]GG 5569 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaII BanII BbsI BbvI BclI BcnI BfaI BmyI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspLU11I BspMI BsrGI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraI EarI EcoRII EcoT22I FbaI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII