##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AflIII      
AgeI        Alw44I      Aor51HI     ApaLI       Asp718I     
AspI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BlnI        BsaAI       BsaHI       
BsiEI       BsiWI       Bsp1407I    BspDI       BspLU11I    
BsrGI       Bst1107I    BstBI       BstXI       BstZ17I     
ClaI        CpoI        DrdI        EagI        EclXI       
Eco47III    Eco52I      EcoRI       EcoRV       EcoT22I     
FseI        FspI        Hin1I       KasI        KpnI        
KspI        MluI        MluNI       MscI        NaeI        
NarI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SphI        Sse8387I    SspBI       SwaI        
Tth111I     XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AscI         4291  GGCGCGCC             GG^CGCGCC
BbvCI        2047  GCTGAGG              CCTCAGC(-5/-2)
BcgI           94  CGATTCTCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI         1146  GCCTCCAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI        3472  GATCCATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrFI        4010  ACCGGC               [GA]^CCGG[CT]
BssHII       4292  GCGCGC               G^CGCGC
BssSI         508  CACGAG               CACGAG(-5/-1)
Cfr10I       4010  ACCGGC               [GA]^CCGG[CT]
DraIII       1751  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         5490  CGGCCA               [CT]^GGCC[GA]
HaeII         306  GGCGCT               [GA]GCGC^[CT]
HgaI          513  GCGTC                GACGC(5/10)
HincII       3919  GTTAAC               GT[CT]^[GA]AC
HindII       3919  GTTAAC               GT[CT]^[GA]AC
HpaI         3919  GTTAAC               GTT^AAC
MamI         3472  GATCCATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1559  CAATTG               C^AATTG
MunI         1559  CAATTG               C^AATTG
NcoI          592  CCATGG               C^CATGG
PaeR7I       2507  CTCGAG               C^TCGAG
PflMI        5251  CCAGGATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         662  AGGACCC              [GA]G^G[AT]CC[CT]
SacI         3025  GAGCTC               GAGCT^C
SapI         4572  GAAGAGC              GCTCTTC(1/4)
SpeI          627  ACTAGT               A^CTAGT
SspI         1079  AATATT               AAT^ATT
Van91I       5251  CCAGGATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         2507  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AccI         4222  GTCTAC               GT^[AC][GT]AC
             4532  GTAGAC              
AccII         263  CGCG                 CG^CG
             4293  CGCG                
AccIII       1977  TCCGGA               T^CCGGA
             5024  TCCGGA              
AflII        3544  CTTAAG               C^TTAAG
             4614  CTTAAG              
AhdI         1225  GACATCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1674  GACCCCAGGTC         
AseI         1987  ATTAAT               AT^TAAT
             5185  ATTAAT              
AsnI         1987  ATTAAT               AT^TAAT
             5185  ATTAAT              
Asp700I       530  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4275  GAAACCCTTC          
AspEI        1225  GACATCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1674  GACCCCAGGTC         
AvaI         2507  CTCGAG               C^[CT]CG[GA]G
             4897  CCCGAG              
BanI         3124  GGCACC               G^G[CT][GA]CC
             4105  GGTGCC              
BciVI        5154  GGATAC               GTATCC(6/5)
             1101  GTATCC              
BclI          783  TGATCA               T^GATCA
             4941  TGATCA              
BfrI         3544  CTTAAG               C^TTAAG
             4614  CTTAAG              
BglII         448  AGATCT               A^GATCT
             5339  AGATCT              
BseAI        1977  TCCGGA               T^CCGGA
             5024  TCCGGA              
BsmBI         514  CGTCTC               CGTCTC(1/5)
             5082  CGTCTC              
BsoBI        2507  CTCGAG               C^[CT]CG[GA]G
             4897  CCCGAG              
BspEI        1977  TCCGGA               T^CCGGA
             5024  TCCGGA              
BsrBI         539  CCGCTC               CCGCTC(-3/-3)
              667  CCGCTC              
BstEII       1446  GGTTACC              G^GT[ACGT]ACC
             1723  GGTCACC             
BstPI        1446  GGTTACC              G^GT[ACGT]ACC
             1723  GGTCACC             
BstUI         263  CGCG                 CG^CG
             4293  CGCG                
Eam1105I     1225  GACATCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1674  GACCCCAGGTC         
EcoO65I      1446  GGTTACC              G^GT[ACGT]ACC
             1723  GGTCACC             
FbaI          783  TGATCA               T^GATCA
             4941  TGATCA              
MaeII        1778  ACGT                 A^CGT
             4877  ACGT                
MroI         1977  TCCGGA               T^CCGGA
             5024  TCCGGA              
MvnI          263  CGCG                 CG^CG
             4293  CGCG                
NspI         3676  GCATGT               [GA]CATG^[CT]
             4751  ACATGC              
PshBI        1987  ATTAAT               AT^TAAT
             5185  ATTAAT              
SexAI        2307  ACCAGGT              A^CC[AT]GGT
             2477  ACCAGGT             
XbaI         1188  TCTAGA               T^CTAGA
             3507  TCTAGA              
XcmI          763  CCAGCAGACCCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              991  CCAGCTCAGCGTTGG     
XmnI          530  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4275  GAAACCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  111 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       AocI        ApaI        ApoI        AspHI       
AvaII       BanII       BbsI        BbvI        BcnI        
BfaI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    BspHI       BspMI       
BsrDI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstYI       Bsu36I      Cac8I       CelII       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DraII       DsaI        EarI        Eco81I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeI        
NdeII       NlaIII      NlaIV       PleI        PshAI       
PstI        PvuII       RcaI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII