################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AatII Acc65I AclI AcyI AflIII AgeI Alw44I Aor51HI ApaLI Asp718I AspI AviII AvrII BalI BamHI BbeI BbrPI BlnI BsaAI BsaHI BsiEI BsiWI Bsp1407I BspDI BspLU11I BsrGI Bst1107I BstBI BstXI BstZ17I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FseI FspI Hin1I KasI KpnI KspI MluI MluNI MscI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AscI 4291 GGCGCGCC GG^CGCGCC BbvCI 2047 GCTGAGG CCTCAGC(-5/-2) BcgI 94 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 1146 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 3472 GATCCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsrFI 4010 ACCGGC [GA]^CCGG[CT] BssHII 4292 GCGCGC G^CGCGC BssSI 508 CACGAG CACGAG(-5/-1) Cfr10I 4010 ACCGGC [GA]^CCGG[CT] DraIII 1751 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 5490 CGGCCA [CT]^GGCC[GA] HaeII 306 GGCGCT [GA]GCGC^[CT] HgaI 513 GCGTC GACGC(5/10) HincII 3919 GTTAAC GT[CT]^[GA]AC HindII 3919 GTTAAC GT[CT]^[GA]AC HpaI 3919 GTTAAC GTT^AAC MamI 3472 GATCCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1559 CAATTG C^AATTG MunI 1559 CAATTG C^AATTG NcoI 592 CCATGG C^CATGG PaeR7I 2507 CTCGAG C^TCGAG PflMI 5251 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 662 AGGACCC [GA]G^G[AT]CC[CT] SacI 3025 GAGCTC GAGCT^C SapI 4572 GAAGAGC GCTCTTC(1/4) SpeI 627 ACTAGT A^CTAGT SspI 1079 AATATT AAT^ATT Van91I 5251 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 2507 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccI 4222 GTCTAC GT^[AC][GT]AC 4532 GTAGAC AccII 263 CGCG CG^CG 4293 CGCG AccIII 1977 TCCGGA T^CCGGA 5024 TCCGGA AflII 3544 CTTAAG C^TTAAG 4614 CTTAAG AhdI 1225 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1674 GACCCCAGGTC AseI 1987 ATTAAT AT^TAAT 5185 ATTAAT AsnI 1987 ATTAAT AT^TAAT 5185 ATTAAT Asp700I 530 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4275 GAAACCCTTC AspEI 1225 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1674 GACCCCAGGTC AvaI 2507 CTCGAG C^[CT]CG[GA]G 4897 CCCGAG BanI 3124 GGCACC G^G[CT][GA]CC 4105 GGTGCC BciVI 5154 GGATAC GTATCC(6/5) 1101 GTATCC BclI 783 TGATCA T^GATCA 4941 TGATCA BfrI 3544 CTTAAG C^TTAAG 4614 CTTAAG BglII 448 AGATCT A^GATCT 5339 AGATCT BseAI 1977 TCCGGA T^CCGGA 5024 TCCGGA BsmBI 514 CGTCTC CGTCTC(1/5) 5082 CGTCTC BsoBI 2507 CTCGAG C^[CT]CG[GA]G 4897 CCCGAG BspEI 1977 TCCGGA T^CCGGA 5024 TCCGGA BsrBI 539 CCGCTC CCGCTC(-3/-3) 667 CCGCTC BstEII 1446 GGTTACC G^GT[ACGT]ACC 1723 GGTCACC BstPI 1446 GGTTACC G^GT[ACGT]ACC 1723 GGTCACC BstUI 263 CGCG CG^CG 4293 CGCG Eam1105I 1225 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1674 GACCCCAGGTC EcoO65I 1446 GGTTACC G^GT[ACGT]ACC 1723 GGTCACC FbaI 783 TGATCA T^GATCA 4941 TGATCA MaeII 1778 ACGT A^CGT 4877 ACGT MroI 1977 TCCGGA T^CCGGA 5024 TCCGGA MvnI 263 CGCG CG^CG 4293 CGCG NspI 3676 GCATGT [GA]CATG^[CT] 4751 ACATGC PshBI 1987 ATTAAT AT^TAAT 5185 ATTAAT SexAI 2307 ACCAGGT A^CC[AT]GGT 2477 ACCAGGT XbaI 1188 TCTAGA T^CTAGA 3507 TCTAGA XcmI 763 CCAGCAGACCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 991 CCAGCTCAGCGTTGG XmnI 530 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4275 GAAACCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaII BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV PleI PshAI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII