##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccIII AcyI AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
AspEI AvaI AviII BamHI BanII
BbeI BbrPI BcgI BciVI BclI
BglI BsaAI BsaHI BsaWI BseAI
BsiWI BsmBI BsoBI BspDI BspEI
BssHII BssSI Bst1107I BstBI BstZ17I
ClaI CpoI DrdI Eam1105I Eco47III
FbaI FseI FspI HaeII HgaI
Hin1I HpaI KasI KspI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NheI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
PstI PvuI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 2121 GGTACC G^GTACC
AclI 2187 AACGTT AA^CGTT
Alw44I 1503 GTGCAC G^TGCAC
AlwNI 3920 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1503 GTGCAC G^TGCAC
Asp718I 2121 GGTACC G^GTACC
AspI 3190 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BlpI 136 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 136 GCTGAGC GC^T[ACGT]AGC
BsrFI 2968 GCCGGT [GA]^CCGG[CT]
BstAPI 4834 GCATAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 793 GGTCACC G^GT[ACGT]ACC
BstPI 793 GGTCACC G^GT[ACGT]ACC
CelII 136 GCTGAGC GC^T[ACGT]AGC
CfoI 5344 GCGC GCG^C
Cfr10I 2968 GCCGGT [GA]^CCGG[CT]
DsaI 4405 CCATGG C^C[GA][CT]GG
EcoO65I 793 GGTCACC G^GT[ACGT]ACC
HhaI 5344 GCGC GCG^C
HinP1I 5344 GCGC G^CGC
HincII 4311 GTCAAC GT[CT]^[GA]AC
HindII 4311 GTCAAC GT[CT]^[GA]AC
KpnI 2121 GGTACC GGTAC^C
NcoI 4405 CCATGG C^CATGG
NdeI 5445 CATATG CA^TATG
NotI 5693 GCGGCCGC GC^GGCCGC
NruI 5687 TCGCGA TCG^CGA
PflFI 3190 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2758 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 5399 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2187 AACGTT AA^CGTT
SapI 334 GCTCTTC GCTCTTC(1/4)
ScaI 2556 AGTACT AGT^ACT
SpeI 2112 ACTAGT A^CTAGT
SwaI 3634 ATTTAAAT ATTT^AAAT
Tth111I 3190 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2758 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 2627 GTAGAC GT^[AC][GT]AC
4116 GTCTAC
AccII 5343 CGCG CG^CG
5688 CGCG
AflII 2140 CTTAAG C^TTAAG
3446 CTTAAG
AflIII 100 ACATGT A^C[GA][CT]GT
4142 ACATGT
AvaII 1892 GGACC G^G[AT]CC
3972 GGTCC
AvrII 2359 CCTAGG C^CTAGG
2608 CCTAGG
BbvCI 2783 CCTCAGC CCTCAGC(-5/-2)
3280 CCTCAGC
BcnI 2118 CCGGG CC^[GC]GG
2964 CCCGG
BfrI 2140 CTTAAG C^TTAAG
3446 CTTAAG
BglII 318 AGATCT A^GATCT
4548 AGATCT
BlnI 2359 CCTAGG C^CTAGG
2608 CCTAGG
BsaBI 313 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1378 GATACACATC
BseRI 2753 CTCCTC GAGGAG(10/8)
4 GAGGAG
BsiEI 2966 CGGCCG CG[GA][CT]^CG
5694 CGGCCG
BspHI 1635 TCATGA T^CATGA
3501 TCATGA
BspLU11I 100 ACATGT A^CATGT
4142 ACATGT
BsrBI 2094 CCGCTC CCGCTC(-3/-3)
5691 GAGCGG
BstUI 5343 CGCG CG^CG
5688 CGCG
EagI 2966 CGGCCG C^GGCCG
5694 CGGCCG
EclXI 2966 CGGCCG C^GGCCG
5694 CGGCCG
Eco52I 2966 CGGCCG C^GGCCG
5694 CGGCCG
EcoNI 771 CCTTACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5499 CCTGCCTCAGG
EcoRV 3717 GATATC GAT^ATC
3908 GATATC
EcoT22I 282 ATGCAT ATGCA^T
3674 ATGCAT
FauI 2829 CCCGC CCCGC(4/6)
5168 GCGGG
MaeII 1898 ACGT A^CGT
2188 ACGT
MamI 313 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1378 GATACACATC
MvnI 5343 CGCG CG^CG
5688 CGCG
NciI 2118 CCGGG CC^[GC]GG
2964 CCCGG
NsiI 282 ATGCAT ATGCA^T
3674 ATGCAT
PpuMI 1891 GGGACCT [GA]G^G[AT]CC[CT]
3971 GGGTCCC
RcaI 1635 TCATGA T^CATGA
3501 TCATGA
SphI 284 GCATGC GCATG^C
2813 GCATGC
SspI 4197 AATATT AAT^ATT
4867 AATATT
TaqI 3385 TCGA T^CGA
4340 TCGA
TthHB8I 3385 TCGA T^CGA
4340 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI Asp700I AspHI BalI
BanI BbsI BbvI BfaI BmyI
BpmI BpuAI BsaI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspMI BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraI DraII DraIII
EaeI EarI Eco81I EcoO109I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XbaI XhoII
XmnI