##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AhdI Alw44I AocI Aor51HI ApaLI
AscI AspEI AviII AvrII BbeI
BbvCI BciVI BcnI BlnI BsaBI
BsiWI BspDI BspMI BssHII BssSI
BstBI Bsu36I ClaI CpoI Eam1105I
Eco47III Eco81I FseI FspI HaeII
HpaI KasI KspI MamI MluI
NaeI NarI NciI NdeI NgoMIV
NheI NspV PacI PaeR7I PmeI
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SmaI SnaBI SphI Sse8387I SspI
SwaI TaqI TthHB8I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 2064 GGTACC G^GTACC
AccIII 2697 TCCGGA T^CCGGA
AflII 4031 CTTAAG C^TTAAG
AgeI 2526 ACCGGT A^CCGGT
ApaI 2000 GGGCCC GGGCC^C
AseI 4858 ATTAAT AT^TAAT
AsnI 4858 ATTAAT AT^TAAT
Asp700I 961 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2064 GGTACC G^GTACC
BbrPI 3854 CACGTG CAC^GTG
BfrI 4031 CTTAAG C^TTAAG
BglII 115 AGATCT A^GATCT
BsaAI 3854 CACGTG [CT]AC^GT[GA]
BseAI 2697 TCCGGA T^CCGGA
BsmBI 1985 CGTCTC CGTCTC(1/5)
BspEI 2697 TCCGGA T^CCGGA
BspLU11I 785 ACATGT A^CATGT
BsrBI 5126 GAGCGG CCGCTC(-3/-3)
BsrFI 2526 ACCGGT [GA]^CCGG[CT]
Bst1107I 3894 GTATAC GTA^TAC
BstEII 4652 GGTCACC G^GT[ACGT]ACC
BstPI 4652 GGTCACC G^GT[ACGT]ACC
BstZ17I 3894 GTATAC GTA^TAC
CfoI 2886 GCGC GCG^C
Cfr10I 2526 ACCGGT [GA]^CCGG[CT]
DraI 4297 TTTAAA TTT^AAA
DraIII 2150 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2950 GACAGTCTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 5129 CGGCCG C^GGCCG
EclXI 5129 CGGCCG C^GGCCG
Eco52I 5129 CGGCCG C^GGCCG
EcoO65I 4652 GGTCACC G^GT[ACGT]ACC
EcoRI 3779 GAATTC G^AATTC
EcoRV 3735 GATATC GAT^ATC
EcoT22I 4785 ATGCAT ATGCA^T
HhaI 2886 GCGC GCG^C
HinP1I 2886 GCGC G^CGC
KpnI 2064 GGTACC GGTAC^C
MfeI 4712 CAATTG C^AATTG
MroI 2697 TCCGGA T^CCGGA
MunI 4712 CAATTG C^AATTG
NotI 5128 GCGGCCGC GC^GGCCGC
NruI 5122 TCGCGA TCG^CGA
NsiI 4785 ATGCAT ATGCA^T
PflMI 3698 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2526 ACCGGT A^CCGGT
PmaCI 3854 CACGTG CAC^GTG
PmlI 3854 CACGTG CAC^GTG
PshAI 1351 GACTATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4858 ATTAAT AT^TAAT
SgrAI 2525 CACCGGTG C[GA]^CCGG[CT]G
StuI 443 AGGCCT AGG^CCT
Van91I 3698 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 5116 TCTAGA T^CTAGA
XmnI 961 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AatII 421 GACGTC GACGT^C
2844 GACGTC
AccI 3894 GTATAC GT^[AC][GT]AC
4707 GTAGAC
AccII 528 CGCG CG^CG
5123 CGCG
AclI 1303 AACGTT AA^CGTT
3639 AACGTT
AcsI 3145 GAATTT [GA]^AATT[CT]
3779 GAATTC
AcyI 421 GACGTC G[GA]^CG[CT]C
2844 GACGTC
ApoI 3145 GAATTT [GA]^AATT[CT]
3779 GAATTC
AspI 418 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1515 GACAGAGTC
BamHI 1454 GGATCC G^GATCC
1567 GGATCC
BcgI 420 CGACGTCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3229 GCAGTTTTCTCG
BglI 220 GCCTTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2661 GCCCAAGTGGC
BsaHI 421 GACGTC G[GA]^CG[CT]C
2844 GACGTC
BsaWI 2526 ACCGGT [AT]^CCGG[AT]
2697 TCCGGA
BsiEI 2537 CGGTCG CG[GA][CT]^CG
5129 CGGCCG
BsmI 1980 GAATGC GAATGC(1/-1)
1992 GCATTC
BspHI 3731 TCATGA T^CATGA
4572 TCATGA
BstAPI 1918 GCACCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2149 GCACCGTGTGC
BstUI 528 CGCG CG^CG
5123 CGCG
BstXI 2330 CCAAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3280 CCACAACAATGG
HapII 2527 CCGG C^CGG
2698 CCGG
Hin1I 421 GACGTC G[GA]^CG[CT]C
2844 GACGTC
HincII 1300 GTCAAC GT[CT]^[GA]AC
3061 GTCAAC
HindII 1300 GTCAAC GT[CT]^[GA]AC
3061 GTCAAC
HindIII 997 AAGCTT A^AGCTT
4553 AAGCTT
HpaII 2527 CCGG C^CGG
2698 CCGG
MspI 2527 CCGG C^CGG
2698 CCGG
MvnI 528 CGCG CG^CG
5123 CGCG
PflFI 418 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1515 GACAGAGTC
Psp1406I 1303 AACGTT AA^CGTT
3639 AACGTT
RcaI 3731 TCATGA T^CATGA
4572 TCATGA
SacI 253 GAGCTC GAGCT^C
1677 GAGCTC
SpeI 4336 ACTAGT A^CTAGT
5110 ACTAGT
Tth111I 418 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1515 GACAGAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BclI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HgaI HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MvaI MwoI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspBI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII