##################################
# NO CUT ENZYMES (   79 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AgeI        AhdI        AscI        Asp700I     Asp718I     
AspEI       AviII       BbeI        BbrPI       BcgI        
BglII       BlpI        Bpu1102I    BsaHI       BsaWI       
BseAI       BsiEI       BspDI       BspEI       BspMI       
BsrBI       BsrFI       BssHII      BssSI       BstBI       
CelII       Cfr10I      ClaI        CpoI        EagI        
Eam1105I    EclXI       Eco52I      EcoRV       FseI        
FspI        Hin1I       HpaI        KasI        KpnI        
KspI        MfeI        MluI        MroI        MunI        
NaeI        NarI        NgoMIV      NotI        NruI        
NspV        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PvuI        RsrII       
SacII       SalI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SwaI        XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

Alw44I       1490  GTGCAC               G^TGCAC
ApaLI        1490  GTGCAC               G^TGCAC
BanI          137  GGCACC               G^G[CT][GA]CC
BciVI        1630  GGATAC               GTATCC(6/5)
BclI          445  TGATCA               T^GATCA
BglI         2162  GCCCCCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         170  TACGTA               [CT]AC^GT[GA]
BsaBI        3383  GATGATTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI        4021  CGTCTC               CGTCTC(1/5)
BstEII       3444  GGTCACC              G^GT[ACGT]ACC
BstPI        3444  GGTCACC              G^GT[ACGT]ACC
DraIII       1660  CACCGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1534  GACTGTTTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         1972  CCATGG               C^C[GA][CT]GG
EcoNI        3155  CCTTCCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      3444  GGTCACC              G^GT[ACGT]ACC
EcoT22I      1281  ATGCAT               ATGCA^T
FbaI          445  TGATCA               T^GATCA
HincII        788  GTCAAC               GT[CT]^[GA]AC
HindII        788  GTCAAC               GT[CT]^[GA]AC
MamI         3383  GATGATTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI         1972  CCATGG               C^CATGG
NdeI         3392  CATATG               CA^TATG
NheI         2983  GCTAGC               G^CTAGC
NsiI         1281  ATGCAT               ATGCA^T
PacI         1859  TTAATTAA             TTAAT^TAA
PleI         3182  GAGTC                GAGTC(4/5)
PpuMI        2437  GGGTCCT              [GA]G^G[AT]CC[CT]
SacI         1913  GAGCTC               GAGCT^C
SapI         3665  GCTCTTC              GCTCTTC(1/4)
SbfI         1072  CCTGCAGG             CCTGCA^GG
SnaBI         170  TACGTA               TAC^GTA
SpeI         4285  ACTAGT               A^CTAGT
SphI         3896  GCATGC               GCATG^C
Sse8387I     1072  CCTGCAGG             CCTGCA^GG
SspI         4416  AATATT               AAT^ATT
StuI         4319  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   43 enzymes) #
###################################

AccI         3542  GTATAC               GT^[AC][GT]AC
             4931  GTATAC              
AccII          75  CGCG                 CG^CG
               77  CGCG                
AflII         725  CTTAAG               C^TTAAG
             4226  CTTAAG              
AlwNI         684  CAGTCACTG            CAG[ACGT][ACGT][ACGT]^CTG
             2269  CAGTGGCTG           
AocI          179  CCTGAGG              CC^T[ACGT]AGG
             3159  CCTGAGG             
Aor51HI       867  AGCGCT               AGC^GCT
             2025  AGCGCT              
ApaI         2000  GGGCCC               GGGCC^C
             2019  GGGCCC              
AspI         2955  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4016  GACCTCGTC           
AvaI           53  CCCGAG               C^[CT]CG[GA]G
             3917  CTCGGG              
AvrII        2059  CCTAGG               C^CTAGG
             2332  CCTAGG              
BamHI        3677  GGATCC               G^GATCC
             3937  GGATCC              
BfrI          725  CTTAAG               C^TTAAG
             4226  CTTAAG              
BlnI         2059  CCTAGG               C^CTAGG
             2332  CCTAGG              
BsaI         3825  GAGACC               GGTCTC(1/5)
             4754  GAGACC              
BsiWI         168  CGTACG               C^GTACG
              172  CGTACG              
BsmI         1279  GAATGC               GAATGC(1/-1)
             1764  GAATGC              
BsoBI          53  CCCGAG               C^[CT]CG[GA]G
             3917  CTCGGG              
Bsp1407I     4084  TGTACA               T^GTACA
             4667  TGTACA              
BspHI        2507  TCATGA               T^CATGA
             4613  TCATGA              
BspLU11I     3696  ACATGT               A^CATGT
             4206  ACATGT              
BsrDI        1704  CATTGC               GCAATG(2/0)
             4363  CATTGC              
BsrGI        4084  TGTACA               T^GTACA
             4667  TGTACA              
Bst1107I     3542  GTATAC               GTA^TAC
             4931  GTATAC              
BstAPI       1483  GCAGCTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3067  GCACTCCCTGC         
BstUI          75  CGCG                 CG^CG
               77  CGCG                
BstXI        3171  CCATTCCTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3775  CCAGCACTTTGG        
BstZ17I      3542  GTATAC               GTA^TAC
             4931  GTATAC              
Bsu36I        179  CCTGAGG              CC^T[ACGT]AGG
             3159  CCTGAGG             
Eco47III      867  AGCGCT               AGC^GCT
             2025  AGCGCT              
Eco81I        179  CCTGAGG              CC^T[ACGT]AGG
             3159  CCTGAGG             
EcoRI        1514  GAATTC               G^AATTC
             4673  GAATTC              
FauI         2202  CCCGC                CCCGC(4/6)
             3128  GCGGG               
HaeII         867  AGCGCT               [GA]GCGC^[CT]
             2025  AGCGCT              
HindIII      2280  AAGCTT               A^AGCTT
             3235  AAGCTT              
MvnI           75  CGCG                 CG^CG
               77  CGCG                
PflFI        2955  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4016  GACCTCGTC           
PflMI        2268  CCAGTGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2723  CCAGTCATTGG         
PstI         1073  CTGCAG               CTGCA^G
             1339  CTGCAG              
RcaI         2507  TCATGA               T^CATGA
             4613  TCATGA              
ScaI          274  AGTACT               AGT^ACT
             1130  AGTACT              
SspBI        4084  TGTACA               T^GTACA
             4667  TGTACA              
Tth111I      2955  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4016  GACCTCGTC           
Van91I       2268  CCAGTGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2723  CCAGTCATTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   99 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        ApoI        AseI        AsnI        AspHI       
AvaII       BalI        BanII       BbsI        BbvCI       
BbvI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PshBI       
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII