################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI AhdI AscI Asp700I Asp718I AspEI AviII BbeI BbrPI BcgI BglII BlpI Bpu1102I BsaHI BsaWI BseAI BsiEI BspDI BspEI BspMI BsrBI BsrFI BssHII BssSI BstBI CelII Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco52I EcoRV FseI FspI Hin1I HpaI KasI KpnI KspI MfeI MluI MroI MunI NaeI NarI NgoMIV NotI NruI NspV PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SexAI SfiI SfoI SfuI SgrAI SmaI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### Alw44I 1490 GTGCAC G^TGCAC ApaLI 1490 GTGCAC G^TGCAC BanI 137 GGCACC G^G[CT][GA]CC BciVI 1630 GGATAC GTATCC(6/5) BclI 445 TGATCA T^GATCA BglI 2162 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 170 TACGTA [CT]AC^GT[GA] BsaBI 3383 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 4021 CGTCTC CGTCTC(1/5) BstEII 3444 GGTCACC G^GT[ACGT]ACC BstPI 3444 GGTCACC G^GT[ACGT]ACC DraIII 1660 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1534 GACTGTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1972 CCATGG C^C[GA][CT]GG EcoNI 3155 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 3444 GGTCACC G^GT[ACGT]ACC EcoT22I 1281 ATGCAT ATGCA^T FbaI 445 TGATCA T^GATCA HincII 788 GTCAAC GT[CT]^[GA]AC HindII 788 GTCAAC GT[CT]^[GA]AC MamI 3383 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 1972 CCATGG C^CATGG NdeI 3392 CATATG CA^TATG NheI 2983 GCTAGC G^CTAGC NsiI 1281 ATGCAT ATGCA^T PacI 1859 TTAATTAA TTAAT^TAA PleI 3182 GAGTC GAGTC(4/5) PpuMI 2437 GGGTCCT [GA]G^G[AT]CC[CT] SacI 1913 GAGCTC GAGCT^C SapI 3665 GCTCTTC GCTCTTC(1/4) SbfI 1072 CCTGCAGG CCTGCA^GG SnaBI 170 TACGTA TAC^GTA SpeI 4285 ACTAGT A^CTAGT SphI 3896 GCATGC GCATG^C Sse8387I 1072 CCTGCAGG CCTGCA^GG SspI 4416 AATATT AAT^ATT StuI 4319 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 43 enzymes) # ################################### AccI 3542 GTATAC GT^[AC][GT]AC 4931 GTATAC AccII 75 CGCG CG^CG 77 CGCG AflII 725 CTTAAG C^TTAAG 4226 CTTAAG AlwNI 684 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG 2269 CAGTGGCTG AocI 179 CCTGAGG CC^T[ACGT]AGG 3159 CCTGAGG Aor51HI 867 AGCGCT AGC^GCT 2025 AGCGCT ApaI 2000 GGGCCC GGGCC^C 2019 GGGCCC AspI 2955 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4016 GACCTCGTC AvaI 53 CCCGAG C^[CT]CG[GA]G 3917 CTCGGG AvrII 2059 CCTAGG C^CTAGG 2332 CCTAGG BamHI 3677 GGATCC G^GATCC 3937 GGATCC BfrI 725 CTTAAG C^TTAAG 4226 CTTAAG BlnI 2059 CCTAGG C^CTAGG 2332 CCTAGG BsaI 3825 GAGACC GGTCTC(1/5) 4754 GAGACC BsiWI 168 CGTACG C^GTACG 172 CGTACG BsmI 1279 GAATGC GAATGC(1/-1) 1764 GAATGC BsoBI 53 CCCGAG C^[CT]CG[GA]G 3917 CTCGGG Bsp1407I 4084 TGTACA T^GTACA 4667 TGTACA BspHI 2507 TCATGA T^CATGA 4613 TCATGA BspLU11I 3696 ACATGT A^CATGT 4206 ACATGT BsrDI 1704 CATTGC GCAATG(2/0) 4363 CATTGC BsrGI 4084 TGTACA T^GTACA 4667 TGTACA Bst1107I 3542 GTATAC GTA^TAC 4931 GTATAC BstAPI 1483 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3067 GCACTCCCTGC BstUI 75 CGCG CG^CG 77 CGCG BstXI 3171 CCATTCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3775 CCAGCACTTTGG BstZ17I 3542 GTATAC GTA^TAC 4931 GTATAC Bsu36I 179 CCTGAGG CC^T[ACGT]AGG 3159 CCTGAGG Eco47III 867 AGCGCT AGC^GCT 2025 AGCGCT Eco81I 179 CCTGAGG CC^T[ACGT]AGG 3159 CCTGAGG EcoRI 1514 GAATTC G^AATTC 4673 GAATTC FauI 2202 CCCGC CCCGC(4/6) 3128 GCGGG HaeII 867 AGCGCT [GA]GCGC^[CT] 2025 AGCGCT HindIII 2280 AAGCTT A^AGCTT 3235 AAGCTT MvnI 75 CGCG CG^CG 77 CGCG PflFI 2955 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4016 GACCTCGTC PflMI 2268 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2723 CCAGTCATTGG PstI 1073 CTGCAG CTGCA^G 1339 CTGCAG RcaI 2507 TCATGA T^CATGA 4613 TCATGA ScaI 274 AGTACT AGT^ACT 1130 AGTACT SspBI 4084 TGTACA T^GTACA 4667 TGTACA Tth111I 2955 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4016 GACCTCGTC Van91I 2268 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2723 CCAGTCATTGG ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI AseI AsnI AspHI AvaII BalI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PshBI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII