################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AgeI Aor51HI AscI AseI AsnI AspI AviII BbrPI BbvCI BcgI BsaBI BsrBI BssHII Bst1107I BstZ17I CpoI DrdI EagI EclXI Eco47III Eco52I EcoRV EcoT22I FseI FspI HpaI KspI MamI MfeI MluI MunI NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI PvuI RsrII SacII SalI SbfI SexAI SfiI SgrAI SnaBI SphI Sse8387I SspI StuI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 1703 GGTACC G^GTACC AccI 4684 GTCTAC GT^[AC][GT]AC AccIII 85 TCCGGA T^CCGGA AclI 152 AACGTT AA^CGTT AhdI 315 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1349 CCTCAGG CC^T[ACGT]AGG Asp718I 1703 GGTACC G^GTACC AspEI 315 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 122 GGATCC G^GATCC BciVI 1861 GGATAC GTATCC(6/5) BclI 2369 TGATCA T^GATCA BglI 4380 GCCTTTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 839 GCTCAGC GC^T[ACGT]AGC Bpu1102I 839 GCTCAGC GC^T[ACGT]AGC BsaAI 1866 CACGTA [CT]AC^GT[GA] BseAI 85 TCCGGA T^CCGGA BsiEI 1012 CGGTCG CG[GA][CT]^CG BsiWI 2053 CGTACG C^GTACG Bsp1407I 5193 TGTACA T^GTACA BspDI 3947 ATCGAT AT^CGAT BspEI 85 TCCGGA T^CCGGA BsrGI 5193 TGTACA T^GTACA BssSI 726 CTCGTG CACGAG(-5/-1) BstBI 1343 TTCGAA TT^CGAA Bsu36I 1349 CCTCAGG CC^T[ACGT]AGG CelII 839 GCTCAGC GC^T[ACGT]AGC ClaI 3947 ATCGAT AT^CGAT Eam1105I 315 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1349 CCTCAGG CC^T[ACGT]AGG FbaI 2369 TGATCA T^GATCA KpnI 1703 GGTACC GGTAC^C MroI 85 TCCGGA T^CCGGA NaeI 3790 GCCGGC GCC^GGC NgoMIV 3790 GCCGGC G^CCGGC NspV 1343 TTCGAA TT^CGAA Psp1406I 152 AACGTT AA^CGTT SapI 3353 GCTCTTC GCTCTTC(1/4) SfuI 1343 TTCGAA TT^CGAA SpeI 2641 ACTAGT A^CTAGT SspBI 5193 TGTACA T^GTACA XbaI 4163 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 41 enzymes) # ################################### AatII 1435 GACGTC GACGT^C 1946 GACGTC AccII 1562 CGCG CG^CG 4555 CGCG AflII 1808 CTTAAG C^TTAAG 4183 CTTAAG Alw44I 4780 GTGCAC G^TGCAC 4969 GTGCAC AlwNI 3398 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG 3763 CAGCCTCTG ApaI 3709 GGGCCC GGGCC^C 4906 GGGCCC ApaLI 4780 GTGCAC G^TGCAC 4969 GTGCAC AvrII 3408 CCTAGG C^CTAGG 3546 CCTAGG BbeI 196 GGCGCC GGCGC^C 4193 GGCGCC BfrI 1808 CTTAAG C^TTAAG 4183 CTTAAG BglII 464 AGATCT A^GATCT 4579 AGATCT BlnI 3408 CCTAGG C^CTAGG 3546 CCTAGG BsgI 3659 GTGCAG GTGCAG(16/14) 3723 GTGCAG BsmI 4867 GAATGC GAATGC(1/-1) 302 GCATTC BspLU11I 2711 ACATGT A^CATGT 3866 ACATGT BsrDI 4134 CATTGC GCAATG(2/0) 4679 GCAATG BstAPI 302 GCATTCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 864 GCAAAAGCTGC BstEII 998 GGTAACC G^GT[ACGT]ACC 2084 GGTTACC BstPI 998 GGTAACC G^GT[ACGT]ACC 2084 GGTTACC BstUI 1562 CGCG CG^CG 4555 CGCG BstXI 1624 CCAACGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2074 CCAAGCAGCTGG DraIII 230 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG 4022 CACCGTGTG EcoNI 561 CCTGAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3403 CCTGGCCTAGG EcoO65I 998 GGTAACC G^GT[ACGT]ACC 2084 GGTTACC EcoRI 384 GAATTC G^AATTC 4942 GAATTC HincII 942 GTTGAC GT[CT]^[GA]AC 2352 GTCAAC HindII 942 GTTGAC GT[CT]^[GA]AC 2352 GTCAAC HindIII 1317 AAGCTT A^AGCTT 3924 AAGCTT KasI 196 GGCGCC G^GCGCC 4193 GGCGCC MvnI 1562 CGCG CG^CG 4555 CGCG NarI 196 GGCGCC GG^CGCC 4193 GGCGCC NcoI 922 CCATGG C^CATGG 2782 CCATGG PflMI 707 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1689 CCAGGGGATGG PpuMI 1996 GGGACCC [GA]G^G[AT]CC[CT] 4358 AGGACCT ScaI 1042 AGTACT AGT^ACT 3190 AGTACT SfoI 196 GGCGCC GGC^GCC 4193 GGCGCC SmaI 1447 CCCGGG CCC^GGG 4151 CCCGGG Van91I 707 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1689 CCAGGGGATGG XcmI 1909 CCAAGTCTGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4131 CCACATTGCTGTTGG XmaCI 1447 CCCGGG C^CCGGG 4151 CCCGGG XmaI 1447 CCCGGG C^CCGGG 4151 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AciI AcsI AcyI AfaI AflIII AluI AlwI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspHI BspMI BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV NspI PleI PshAI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI