##################################
# NO CUT ENZYMES (   59 enzymes) #
##################################

AgeI        Aor51HI     AscI        AseI        AsnI        
AspI        AviII       BbrPI       BbvCI       BcgI        
BsaBI       BsrBI       BssHII      Bst1107I    BstZ17I     
CpoI        DrdI        EagI        EclXI       Eco47III    
Eco52I      EcoRV       EcoT22I     FseI        FspI        
HpaI        KspI        MamI        MfeI        MluI        
MunI        NheI        NotI        NruI        NsiI        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshBI       PvuI        RsrII       
SacII       SalI        SbfI        SexAI       SfiI        
SgrAI       SnaBI       SphI        Sse8387I    SspI        
StuI        SwaI        Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I       1703  GGTACC               G^GTACC
AccI         4684  GTCTAC               GT^[AC][GT]AC
AccIII         85  TCCGGA               T^CCGGA
AclI          152  AACGTT               AA^CGTT
AhdI          315  GACCCTGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1349  CCTCAGG              CC^T[ACGT]AGG
Asp718I      1703  GGTACC               G^GTACC
AspEI         315  GACCCTGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI         122  GGATCC               G^GATCC
BciVI        1861  GGATAC               GTATCC(6/5)
BclI         2369  TGATCA               T^GATCA
BglI         4380  GCCTTTATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          839  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      839  GCTCAGC              GC^T[ACGT]AGC
BsaAI        1866  CACGTA               [CT]AC^GT[GA]
BseAI          85  TCCGGA               T^CCGGA
BsiEI        1012  CGGTCG               CG[GA][CT]^CG
BsiWI        2053  CGTACG               C^GTACG
Bsp1407I     5193  TGTACA               T^GTACA
BspDI        3947  ATCGAT               AT^CGAT
BspEI          85  TCCGGA               T^CCGGA
BsrGI        5193  TGTACA               T^GTACA
BssSI         726  CTCGTG               CACGAG(-5/-1)
BstBI        1343  TTCGAA               TT^CGAA
Bsu36I       1349  CCTCAGG              CC^T[ACGT]AGG
CelII         839  GCTCAGC              GC^T[ACGT]AGC
ClaI         3947  ATCGAT               AT^CGAT
Eam1105I      315  GACCCTGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1349  CCTCAGG              CC^T[ACGT]AGG
FbaI         2369  TGATCA               T^GATCA
KpnI         1703  GGTACC               GGTAC^C
MroI           85  TCCGGA               T^CCGGA
NaeI         3790  GCCGGC               GCC^GGC
NgoMIV       3790  GCCGGC               G^CCGGC
NspV         1343  TTCGAA               TT^CGAA
Psp1406I      152  AACGTT               AA^CGTT
SapI         3353  GCTCTTC              GCTCTTC(1/4)
SfuI         1343  TTCGAA               TT^CGAA
SpeI         2641  ACTAGT               A^CTAGT
SspBI        5193  TGTACA               T^GTACA
XbaI         4163  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   41 enzymes) #
###################################

AatII        1435  GACGTC               GACGT^C
             1946  GACGTC              
AccII        1562  CGCG                 CG^CG
             4555  CGCG                
AflII        1808  CTTAAG               C^TTAAG
             4183  CTTAAG              
Alw44I       4780  GTGCAC               G^TGCAC
             4969  GTGCAC              
AlwNI        3398  CAGACCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             3763  CAGCCTCTG           
ApaI         3709  GGGCCC               GGGCC^C
             4906  GGGCCC              
ApaLI        4780  GTGCAC               G^TGCAC
             4969  GTGCAC              
AvrII        3408  CCTAGG               C^CTAGG
             3546  CCTAGG              
BbeI          196  GGCGCC               GGCGC^C
             4193  GGCGCC              
BfrI         1808  CTTAAG               C^TTAAG
             4183  CTTAAG              
BglII         464  AGATCT               A^GATCT
             4579  AGATCT              
BlnI         3408  CCTAGG               C^CTAGG
             3546  CCTAGG              
BsgI         3659  GTGCAG               GTGCAG(16/14)
             3723  GTGCAG              
BsmI         4867  GAATGC               GAATGC(1/-1)
              302  GCATTC              
BspLU11I     2711  ACATGT               A^CATGT
             3866  ACATGT              
BsrDI        4134  CATTGC               GCAATG(2/0)
             4679  GCAATG              
BstAPI        302  GCATTCTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              864  GCAAAAGCTGC         
BstEII        998  GGTAACC              G^GT[ACGT]ACC
             2084  GGTTACC             
BstPI         998  GGTAACC              G^GT[ACGT]ACC
             2084  GGTTACC             
BstUI        1562  CGCG                 CG^CG
             4555  CGCG                
BstXI        1624  CCAACGACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2074  CCAAGCAGCTGG        
DraIII        230  CACACCGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4022  CACCGTGTG           
EcoNI         561  CCTGAAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3403  CCTGGCCTAGG         
EcoO65I       998  GGTAACC              G^GT[ACGT]ACC
             2084  GGTTACC             
EcoRI         384  GAATTC               G^AATTC
             4942  GAATTC              
HincII        942  GTTGAC               GT[CT]^[GA]AC
             2352  GTCAAC              
HindII        942  GTTGAC               GT[CT]^[GA]AC
             2352  GTCAAC              
HindIII      1317  AAGCTT               A^AGCTT
             3924  AAGCTT              
KasI          196  GGCGCC               G^GCGCC
             4193  GGCGCC              
MvnI         1562  CGCG                 CG^CG
             4555  CGCG                
NarI          196  GGCGCC               GG^CGCC
             4193  GGCGCC              
NcoI          922  CCATGG               C^CATGG
             2782  CCATGG              
PflMI         707  CCATGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1689  CCAGGGGATGG         
PpuMI        1996  GGGACCC              [GA]G^G[AT]CC[CT]
             4358  AGGACCT             
ScaI         1042  AGTACT               AGT^ACT
             3190  AGTACT              
SfoI          196  GGCGCC               GGC^GCC
             4193  GGCGCC              
SmaI         1447  CCCGGG               CCC^GGG
             4151  CCCGGG              
Van91I        707  CCATGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1689  CCAGGGGATGG         
XcmI         1909  CCAAGTCTGATATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4131  CCACATTGCTGTTGG     
XmaCI        1447  CCCGGG               C^CCGGG
             4151  CCCGGG              
XmaI         1447  CCCGGG               C^CCGGG
             4151  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  117 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AflIII      
AluI        AlwI        ApoI        Asp700I     AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaHI       BsaI        BsaJI       
BsaWI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspHI       BspMI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraI        
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeI        NdeII       
NlaIII      NlaIV       NspI        PleI        PshAI       
PstI        PvuII       RcaI        RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       XmnI