##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AccI AccIII AflII AgeI AscI
AseI AsnI AviII BfrI BsaWI
BseAI BsiWI BsmBI BsmI BspDI
BspEI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DrdI EagI EclXI Eco52I EcoO65I
EcoRV FseI FspI HindIII HpaI
KspI MfeI MluI MroI MunI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
PvuI RsrII SacII SalI SfuI
SgrAI SnaBI SpeI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 22 enzymes) #
###################################
AatII 2256 GACGTC GACGT^C
Acc65I 1846 GGTACC G^GTACC
AclI 5080 AACGTT AA^CGTT
Aor51HI 1873 AGCGCT AGC^GCT
Asp718I 1846 GGTACC G^GTACC
AvrII 2327 CCTAGG C^CTAGG
BbvCI 1928 GCTGAGG CCTCAGC(-5/-2)
BcgI 553 GCAGCACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 2327 CCTAGG C^CTAGG
BsiEI 2592 CGACCG CG[GA][CT]^CG
DraI 4953 TTTAAA TTT^AAA
Eco47III 1873 AGCGCT AGC^GCT
EcoRI 2978 GAATTC G^AATTC
EcoT22I 5137 ATGCAT ATGCA^T
KpnI 1846 GGTACC GGTAC^C
NdeI 5159 CATATG CA^TATG
NsiI 5137 ATGCAT ATGCA^T
Psp1406I 5080 AACGTT AA^CGTT
SexAI 2986 ACCTGGT A^CC[AT]GGT
SfiI 173 GGCCAGCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1990 GCATGC GCATG^C
SspI 870 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 45 enzymes) #
###################################
AhdI 738 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2220 GACCTGAAGTC
Alw44I 3977 GTGCAC G^TGCAC
4788 GTGCAC
ApaLI 3977 GTGCAC G^TGCAC
4788 GTGCAC
Asp700I 1958 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4735 GAATGAGTTC
AspEI 738 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2220 GACCTGAAGTC
AspI 1467 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1638 GACTGTGTC
BamHI 4426 GGATCC G^GATCC
4698 GGATCC
BbeI 2322 GGCGCC GGCGC^C
4032 GGCGCC
BbrPI 530 CACGTG CAC^GTG
5054 CACGTG
BciVI 2927 GGATAC GTATCC(6/5)
5103 GGATAC
BglII 31 AGATCT A^GATCT
3229 AGATCT
BlpI 2078 GCTCAGC GC^T[ACGT]AGC
4796 GCTTAGC
Bpu1102I 2078 GCTCAGC GC^T[ACGT]AGC
4796 GCTTAGC
BsaBI 1166 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3545 GATCCTGATC
Bsp1407I 3520 TGTACA T^GTACA
5058 TGTACA
BspHI 1732 TCATGA T^CATGA
2557 TCATGA
BspLU11I 3418 ACATGT A^CATGT
5170 ACATGT
BsrDI 3896 CATTGC GCAATG(2/0)
3187 GCAATG
BsrGI 3520 TGTACA T^GTACA
5058 TGTACA
CelII 2078 GCTCAGC GC^T[ACGT]AGC
4796 GCTTAGC
DraIII 89 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
3998 CACAGAGTG
Eam1105I 738 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2220 GACCTGAAGTC
EarI 1755 CTCTTC CTCTTC(1/4)
966 GAAGAG
KasI 2322 GGCGCC G^GCGCC
4032 GGCGCC
Ksp632I 1755 CTCTTC CTCTTC(1/4)
966 GAAGAG
MamI 1166 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3545 GATCCTGATC
NaeI 110 GCCGGC GCC^GGC
4078 GCCGGC
NarI 2322 GGCGCC GG^CGCC
4032 GGCGCC
NgoMIV 110 GCCGGC G^CCGGC
4078 GCCGGC
PflFI 1467 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1638 GACTGTGTC
PmaCI 530 CACGTG CAC^GTG
5054 CACGTG
PmlI 530 CACGTG CAC^GTG
5054 CACGTG
RcaI 1732 TCATGA T^CATGA
2557 TCATGA
SacI 99 GAGCTC GAGCT^C
2067 GAGCTC
SapI 966 GAAGAGC GCTCTTC(1/4)
1754 GCTCTTC
SbfI 611 CCTGCAGG CCTGCA^GG
3782 CCTGCAGG
ScaI 973 AGTACT AGT^ACT
1621 AGTACT
SfoI 2322 GGCGCC GGC^GCC
4032 GGCGCC
SmaI 4151 CCCGGG CCC^GGG
4702 CCCGGG
Sse8387I 611 CCTGCAGG CCTGCA^GG
3782 CCTGCAGG
SspBI 3520 TGTACA T^GTACA
5058 TGTACA
Tth111I 1467 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1638 GACTGTGTC
XmaCI 4151 CCCGGG C^CCGGG
4702 CCCGGG
XmaI 4151 CCCGGG C^CCGGG
4702 CCCGGG
XmnI 1958 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4735 GAATGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BclI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII