##################################
# NO CUT ENZYMES (   61 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AscI        
Asp718I     AviII       AvrII       BamHI       BbrPI       
BclI        BlnI        BlpI        Bpu1102I    BseAI       
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspLU11I    BsrGI       BssHII      Bst1107I    BstBI       
BstZ17I     CelII       ClaI        CpoI        EcoRV       
FbaI        FseI        FspI        KpnI        KspI        
MluI        MroI        NaeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PmaCI       
PmlI        PshAI       Psp1406I    RsrII       SacII       
SalI        SbfI        SfiI        SfuI        SmaI        
SnaBI       Sse8387I    SspBI       SwaI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccI         5435  GTCTAC               GT^[AC][GT]AC
AccII        5180  CGCG                 CG^CG
AcyI         6071  GGCGCC               G[GA]^CG[CT]C
AflII        5682  CTTAAG               C^TTAAG
AflIII       2288  ACGTGT               A^C[GA][CT]GT
AgeI          508  ACCGGT               A^CCGGT
AhdI         1781  GACAGAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      5823  AGCGCT               AGC^GCT
AspEI        1781  GACAGAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI         6071  GGCGCC               G^G[CT][GA]CC
BbeI         6071  GGCGCC               GGCGC^C
BcgI         6393  GCAGGAGAATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         5682  CTTAAG               C^TTAAG
BglI          423  GCCCTGTAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        2287  TACGTG               [CT]AC^GT[GA]
BsaBI        1255  GATTAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        6071  GGCGCC               G[GA]^CG[CT]C
BsmBI        1882  GAGACG               CGTCTC(1/5)
BsrBI        1464  GAGCGG               CCGCTC(-3/-3)
BssSI         441  CACGAG               CACGAG(-5/-1)
BstEII        145  GGTCACC              G^GT[ACGT]ACC
BstPI         145  GGTCACC              G^GT[ACGT]ACC
BstUI        5180  CGCG                 CG^CG
DraIII       1498  CACAATGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1807  GACTTTACAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI           73  CGGCCG               C^GGCCG
Eam1105I     1781  GACAGAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI          73  CGGCCG               C^GGCCG
Eco47III     5823  AGCGCT               AGC^GCT
Eco52I         73  CGGCCG               C^GGCCG
EcoO65I       145  GGTCACC              G^GT[ACGT]ACC
Hin1I        6071  GGCGCC               G[GA]^CG[CT]C
HpaI         2614  GTTAAC               GTT^AAC
KasI         6071  GGCGCC               G^GCGCC
MamI         1255  GATTAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          289  CAATTG               C^AATTG
MunI          289  CAATTG               C^AATTG
MvnI         5180  CGCG                 CG^CG
NarI         6071  GGCGCC               GG^CGCC
PaeR7I       2304  CTCGAG               C^TCGAG
PinAI         508  ACCGGT               A^CCGGT
PmeI         5890  GTTTAAAC             GTTT^AAAC
PpuMI        1456  AGGACCC              [GA]G^G[AT]CC[CT]
PvuI         1207  CGATCG               CGAT^CG
SexAI        5615  ACCAGGT              A^CC[AT]GGT
SfoI         6071  GGCGCC               GGC^GCC
SpeI         4727  ACTAGT               A^CTAGT
StuI         1406  AGGCCT               AGG^CCT
XbaI         3410  TCTAGA               T^CTAGA
XhoI         2304  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

ApaI         1187  GGGCCC               GGGCC^C
             1749  GGGCCC              
AseI          692  ATTAAT               AT^TAAT
             1025  ATTAAT              
AsnI          692  ATTAAT               AT^TAAT
             1025  ATTAAT              
Asp700I      1224  GAACAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3110  GAAATTGTTC          
AspI         5614  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             6190  GACTTTGTC           
AvaI         2304  CTCGAG               C^[CT]CG[GA]G
             4241  CCCGAG              
BglII        1156  AGATCT               A^GATCT
             5656  AGATCT              
BsaWI         149  ACCGGA               [AT]^CCGG[AT]
              508  ACCGGT              
BsiEI          73  CGGCCG               CG[GA][CT]^CG
             1207  CGATCG              
BsoBI        2304  CTCGAG               C^[CT]CG[GA]G
             4241  CCCGAG              
BspMI         251  ACCTGC               ACCTGC(4/8)
             3971  GCAGGT              
BsrDI        6447  CATTGC               GCAATG(2/0)
             5647  GCAATG              
CfoI         5824  GCGC                 GCG^C
             6072  GCGC                
EcoNI        4904  CCTCCTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             5447  CCTCAAAGAGG         
FauI         1359  CCCGC                CCCGC(4/6)
             5832  CCCGC               
HaeII        5823  AGCGCT               [GA]GCGC^[CT]
             6071  GGCGCC              
HgaI         1528  GCGTC                GACGC(5/10)
             5181  GCGTC               
HhaI         5824  GCGC                 GCG^C
             6072  GCGC                
HinP1I       5824  GCGC                 G^CGC
             6072  GCGC                
HincII       2614  GTTAAC               GT[CT]^[GA]AC
             3359  GTTGAC              
HindII       2614  GTTAAC               GT[CT]^[GA]AC
             3359  GTTGAC              
NcoI         1590  CCATGG               C^CATGG
             4488  CCATGG              
NdeI         3186  CATATG               CA^TATG
             4372  CATATG              
PflFI        5614  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             6190  GACTTTGTC           
PshBI         692  ATTAAT               AT^TAAT
             1025  ATTAAT              
SacI         4054  GAGCTC               GAGCT^C
             5816  GAGCTC              
SapI         2885  GAAGAGC              GCTCTTC(1/4)
             3326  GAAGAGC             
SgrAI         130  CACCGGCG             C[GA]^CCGG[CT]G
              155  CACCGGCG            
Tth111I      5614  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             6190  GACTTTGTC           
XmnI         1224  GAACAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3110  GAAATTGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  117 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        Alw44I      
AlwI        AlwNI       AocI        ApaLI       ApoI        
AspHI       AvaII       BalI        BanII       BbsI        
BbvCI       BbvI        BciVI       BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspHI       BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstXI       BstYI       Bsu36I      Cac8I       Cfr10I      
Cfr13I      DdeI        DpnII       DraI        DraII       
DsaI        EaeI        EarI        Eco81I      EcoO109I    
EcoRI       EcoRII      EcoT14I     EcoT22I     Fnu4HI      
FokI        HaeIII      HapII       HindIII     HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NsiI        NspI        
PflMI       PleI        PstI        PvuII       RcaI        
RsaI        Sau3AI      Sau96I      ScaI        ScrFI       
SfaNI       SfcI        SmlI        SphI        SspI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoII