##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI Aor51HI AscI Asp718I AspEI
AviII AvrII BamHI BbeI BbrPI
BciVI BclI BlnI BlpI Bpu1102I
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BsrBI BssHII
BssSI BstBI BstEII BstPI CelII
ClaI CpoI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
FauI FbaI FseI FspI HaeII
HgaI Hin1I KasI KpnI KspI
MamI MluI MroI NaeI NarI
NcoI NgoMIV NotI NruI NspV
PacI PinAI PmaCI PmlI PvuI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SpeI Sse8387I StuI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 5714 GTATAC GT^[AC][GT]AC
AclI 2453 AACGTT AA^CGTT
AflII 4345 CTTAAG C^TTAAG
Alw44I 4485 GTGCAC G^TGCAC
ApaI 4340 GGGCCC GGGCC^C
ApaLI 4485 GTGCAC G^TGCAC
Asp700I 467 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4094 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2589 TGGCCA TGG^CCA
BbvCI 3057 CCTCAGC CCTCAGC(-5/-2)
BfrI 4345 CTTAAG C^TTAAG
BglI 4252 GCCACTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmBI 1579 GAGACG CGTCTC(1/5)
BsmI 578 GAATGC GAATGC(1/-1)
BsrFI 365 ACCGGC [GA]^CCGG[CT]
Bst1107I 5714 GTATAC GTA^TAC
BstXI 5492 CCACACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 5714 GTATAC GTA^TAC
Cfr10I 365 ACCGGC [GA]^CCGG[CT]
DraIII 2593 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 201 CCTTTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 5561 GATATC GAT^ATC
MluNI 2589 TGGCCA TGG^CCA
MscI 2589 TGGCCA TGG^CCA
NheI 4470 GCTAGC G^CTAGC
PaeR7I 2104 CTCGAG C^TCGAG
PflFI 4094 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 4861 GTTTAAAC GTTT^AAAC
PshAI 920 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2453 AACGTT AA^CGTT
ScaI 4326 AGTACT AGT^ACT
SmaI 4208 CCCGGG CCC^GGG
SnaBI 1782 TACGTA TAC^GTA
SwaI 3658 ATTTAAAT ATTT^AAAT
Tth111I 4094 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 4563 CCATCCCAGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2104 CTCGAG C^TCGAG
XmaCI 4208 CCCGGG C^CCGGG
XmaI 4208 CCCGGG C^CCGGG
XmnI 467 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 495 CGCG CG^CG
2881 CGCG
AciI 701 CCGC CCGC(-3/-1)
2882 GCGG
AflIII 2361 ACATGT A^C[GA][CT]GT
4196 ACATGT
BanII 4340 GGGCCC G[GA]GC[CT]^C
4918 GGGCTC
BcgI 626 GCAAACTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1376 GCAAAGCCCTCG
BsaAI 1782 TACGTA [CT]AC^GT[GA]
2145 TACGTG
BsaI 1155 GAGACC GGTCTC(1/5)
4734 GAGACC
BseRI 3104 CTCCTC GAGGAG(10/8)
4803 GAGGAG
Bsp1407I 1014 TGTACA T^GTACA
3626 TGTACA
BspLU11I 2361 ACATGT A^CATGT
4196 ACATGT
BsrGI 1014 TGTACA T^GTACA
3626 TGTACA
BstAPI 1190 GCATGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1416 GCACTTTATGC
BstUI 495 CGCG CG^CG
2881 CGCG
CfoI 496 GCGC GCG^C
730 GCGC
EaeI 1517 TGGCCG [CT]^GGCC[GA]
2589 TGGCCA
EcoRI 1083 GAATTC G^AATTC
3919 GAATTC
EcoT22I 4110 ATGCAT ATGCA^T
5026 ATGCAT
HhaI 496 GCGC GCG^C
730 GCGC
HinP1I 496 GCGC G^CGC
730 GCGC
MfeI 864 CAATTG C^AATTG
2428 CAATTG
MspA1I 915 CAGCTG C[AC]G^C[GT]G
2504 CAGCTG
MunI 864 CAATTG C^AATTG
2428 CAATTG
MvnI 495 CGCG CG^CG
2881 CGCG
NsiI 4110 ATGCAT ATGCA^T
5026 ATGCAT
PflMI 713 CCATAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1486 CCAACAGTTGG
PvuII 915 CAGCTG CAG^CTG
2504 CAGCTG
SspBI 1014 TGTACA T^GTACA
3626 TGTACA
Van91I 713 CCATAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1486 CCAACAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
AocI ApoI AseI AsnI AspHI
AvaI AvaII BanI BbsI BbvI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
Cfr13I DdeI DpnII DraI DraII
EarI Eco81I EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HincII
HindII HindIII HinfI HpaI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeI NdeII NlaIII NlaIV
NspI PleI PpuMI PshBI PstI
RcaI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XhoII