##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
Aor51HI ApaI AscI Asp718I AvrII
BamHI BbeI BbrPI BbvCI BcgI
BfrI BlnI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BsrDI BsrFI BssHII BstBI BstEII
BstPI Cfr10I ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI FseI KasI KpnI
MamI MfeI MluI MroI MunI
NaeI NarI NgoMIV NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PvuI RsrII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I SwaI
Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AclI 2258 AACGTT AA^CGTT
AcyI 1055 GACGCC G[GA]^CG[CT]C
AhdI 1932 GACAGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1070 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1932 GACAGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 5382 CCCGAG C^[CT]CG[GA]G
AviII 4681 TGCGCA TGC^GCA
BalI 238 TGGCCA TGG^CCA
BclI 4101 TGATCA T^GATCA
BglI 735 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4943 AGATCT A^GATCT
BlpI 4028 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4028 GCTCAGC GC^T[ACGT]AGC
BsaAI 4520 TACGTG [CT]AC^GT[GA]
BsaHI 1055 GACGCC G[GA]^CG[CT]C
BsmI 1859 GAATGC GAATGC(1/-1)
BsoBI 5382 CCCGAG C^[CT]CG[GA]G
BsrBI 3797 CCGCTC CCGCTC(-3/-3)
BssSI 1045 CACGAG CACGAG(-5/-1)
CelII 4028 GCTCAGC GC^T[ACGT]AGC
Eam1105I 1932 GACAGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 388 GATATC GAT^ATC
FbaI 4101 TGATCA T^GATCA
FspI 4681 TGCGCA TGC^GCA
Hin1I 1055 GACGCC G[GA]^CG[CT]C
KspI 4783 CCGCGG CCGC^GG
MluNI 238 TGGCCA TGG^CCA
MscI 238 TGGCCA TGG^CCA
NheI 463 GCTAGC G^CTAGC
Psp1406I 2258 AACGTT AA^CGTT
SacI 638 GAGCTC GAGCT^C
SacII 4783 CCGCGG CCGC^GG
ScaI 4890 AGTACT AGT^ACT
SpeI 5181 ACTAGT A^CTAGT
XbaI 30 TCTAGA T^CTAGA
XcmI 5193 CCAGAAAGAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1070 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AseI 2623 ATTAAT AT^TAAT
2812 ATTAAT
AsnI 2623 ATTAAT AT^TAAT
2812 ATTAAT
BciVI 154 GTATCC GTATCC(6/5)
2011 GTATCC
BcnI 42 CCCGG CC^[GC]GG
2035 CCCGG
BspHI 1839 TCATGA T^CATGA
4143 TCATGA
DraIII 3728 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
4349 CACCATGTG
EcoT22I 3008 ATGCAT ATGCA^T
3288 ATGCAT
HaeII 2130 GGCGCT [GA]GCGC^[CT]
5245 AGCGCC
HapII 43 CCGG C^CGG
2036 CCGG
HpaII 43 CCGG C^CGG
2036 CCGG
MspI 43 CCGG C^CGG
2036 CCGG
NciI 42 CCCGG CC^[GC]GG
2035 CCCGG
NcoI 989 CCATGG C^CATGG
1761 CCATGG
NdeI 1582 CATATG CA^TATG
2418 CATATG
NsiI 3008 ATGCAT ATGCA^T
3288 ATGCAT
PpuMI 2531 AGGACCC [GA]G^G[AT]CC[CT]
2983 AGGACCC
PshAI 611 GACACTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2582 GACAAGCGTC
PshBI 2623 ATTAAT AT^TAAT
2812 ATTAAT
RcaI 1839 TCATGA T^CATGA
4143 TCATGA
SexAI 1824 ACCTGGT A^CC[AT]GGT
4123 ACCTGGT
SphI 4443 GCATGC GCATG^C
4669 GCATGC
SspI 2092 AATATT AAT^ATT
2843 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaLI ApoI AspHI AspI
AvaII BanI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspLU11I BspMI BsrGI BsrI BssKI
Bst1107I BstAPI BstF5I BstNI BstUI
BstXI BstYI BstZ17I Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HgaI HhaI HinP1I
HincII HindII HindIII HinfI HpaI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MvnI
MwoI NdeII NlaIII NlaIV NspI
PflFI PleI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XhoII