##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI AscI Asp718I
AspEI BamHI BbeI BbrPI BsaAI
BsaHI BseAI BsiWI BsmBI BspDI
BspEI BsrDI BsrFI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco52I EcoT22I FseI Hin1I
KasI KpnI KspI MaeII MfeI
MluI MroI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 1161 GTCTAC GT^[AC][GT]AC
AflII 2424 CTTAAG C^TTAAG
Alw44I 2844 GTGCAC G^TGCAC
Aor51HI 192 AGCGCT AGC^GCT
ApaLI 2844 GTGCAC G^TGCAC
AseI 2332 ATTAAT AT^TAAT
AsnI 2332 ATTAAT AT^TAAT
AspI 4431 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1880 TGCGCA TGC^GCA
BciVI 1387 GTATCC GTATCC(6/5)
BfrI 2424 CTTAAG C^TTAAG
BlpI 3569 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3569 GCTGAGC GC^T[ACGT]AGC
BsaWI 1924 TCCGGT [AT]^CCGG[AT]
BsiEI 1356 CGACCG CG[GA][CT]^CG
BsmI 2658 GCATTC GAATGC(1/-1)
Bsp1407I 3846 TGTACA T^GTACA
BspHI 84 TCATGA T^CATGA
BspMI 4494 GCAGGT ACCTGC(4/8)
BsrBI 473 CCGCTC CCGCTC(-3/-3)
BsrGI 3846 TGTACA T^GTACA
BstAPI 4768 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1090 GGTGACC G^GT[ACGT]ACC
BstPI 1090 GGTGACC G^GT[ACGT]ACC
CelII 3569 GCTGAGC GC^T[ACGT]AGC
Eco47III 192 AGCGCT AGC^GCT
EcoO65I 1090 GGTGACC G^GT[ACGT]ACC
EcoRI 3907 GAATTC G^AATTC
FspI 1880 TGCGCA TGC^GCA
HgaI 2909 GACGC GACGC(5/10)
HincII 1854 GTTAAC GT[CT]^[GA]AC
HindII 1854 GTTAAC GT[CT]^[GA]AC
HpaI 1854 GTTAAC GTT^AAC
NdeI 1904 CATATG CA^TATG
PacI 2333 TTAATTAA TTAAT^TAA
PaeR7I 521 CTCGAG C^TCGAG
PflFI 4431 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1154 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2332 ATTAAT AT^TAAT
RcaI 84 TCATGA T^CATGA
ScaI 1619 AGTACT AGT^ACT
SexAI 681 ACCTGGT A^CC[AT]GGT
SfiI 614 GGCCCCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1593 GCATGC GCATG^C
SspBI 3846 TGTACA T^GTACA
SspI 3666 AATATT AAT^ATT
Tth111I 4431 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 521 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflIII 2576 ACATGT A^C[GA][CT]GT
4440 ACATGT
AlwNI 797 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
878 CAGTGGCTG
Asp700I 229 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1823 GAAGCTTTTC
AvrII 2227 CCTAGG C^CTAGG
2471 CCTAGG
BclI 2008 TGATCA T^GATCA
3797 TGATCA
BglII 776 AGATCT A^GATCT
2286 AGATCT
BlnI 2227 CCTAGG C^CTAGG
2471 CCTAGG
BsaBI 1521 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2009 GATCAAGATC
BspLU11I 2576 ACATGT A^CATGT
4440 ACATGT
EcoRV 1525 GATATC GAT^ATC
1953 GATATC
FbaI 2008 TGATCA T^GATCA
3797 TGATCA
HindIII 1824 AAGCTT A^AGCTT
2111 AAGCTT
MamI 1521 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2009 GATCAAGATC
SmaI 261 CCCGGG CCC^GGG
3447 CCCGGG
XmaCI 261 CCCGGG C^CCGGG
3447 CCCGGG
XmaI 261 CCCGGG C^CCGGG
3447 CCCGGG
XmnI 229 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1823 GAAGCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApaI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XcmI XhoII