################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AvrII BamHI BciVI BclI BlnI BsaBI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BsrGI BssHII Bst1107I BstEII BstPI BstZ17I ClaI CpoI Eam1105I Eco47III EcoO65I EcoRV FbaI FseI HgaI HincII HindII HpaI KpnI KspI MamI MfeI MluI MroI MunI NdeI NotI NruI PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SgrAI SnaBI SpeI SphI Sse8387I SspBI SwaI XhoI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccII 5650 CGCG CG^CG AcyI 3333 GGCGCC G[GA]^CG[CT]C AflII 730 CTTAAG C^TTAAG AviII 2243 TGCGCA TGC^GCA BbeI 3333 GGCGCC GGCGC^C BbrPI 5530 CACGTG CAC^GTG BfrI 730 CTTAAG C^TTAAG BsaHI 3333 GGCGCC G[GA]^CG[CT]C BspHI 2900 TCATGA T^CATGA BstUI 5650 CGCG CG^CG DraI 5474 TTTAAA TTT^AAA DrdI 1544 GACTCGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 4995 GAATTC G^AATTC EcoT22I 5021 ATGCAT ATGCA^T FspI 2243 TGCGCA TGC^GCA Hin1I 3333 GGCGCC G[GA]^CG[CT]C KasI 3333 GGCGCC G^GCGCC MvnI 5650 CGCG CG^CG NaeI 1441 GCCGGC GCC^GGC NarI 3333 GGCGCC GG^CGCC NgoMIV 1441 GCCGGC G^CCGGC NheI 1567 GCTAGC G^CTAGC NsiI 5021 ATGCAT ATGCA^T PmaCI 5530 CACGTG CAC^GTG PmlI 5530 CACGTG CAC^GTG PshAI 3927 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 2900 TCATGA T^CATGA SfoI 3333 GGCGCC GGC^GCC SmaI 2393 CCCGGG CCC^GGG SspI 274 AATATT AAT^ATT StuI 3681 AGGCCT AGG^CCT XmaCI 2393 CCCGGG C^CCGGG XmaI 2393 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AcsI 4995 GAATTC [GA]^AATT[CT] 5084 AAATTT ApoI 4995 GAATTC [GA]^AATT[CT] 5084 AAATTT Asp700I 1122 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5311 GAAGTGCTTC AspI 3808 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3844 GACAAGGTC BbsI 76 GAAGAC GAAGAC(2/6) 3924 GAAGAC BbvCI 4861 CCTCAGC CCTCAGC(-5/-2) 103 GCTGAGG BcgI 477 GCATCAGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2315 GCAAGATGCTCG BglII 2079 AGATCT A^GATCT 2748 AGATCT BpuAI 76 GAAGAC GAAGAC(2/6) 3924 GAAGAC BsaAI 5530 CACGTG [CT]AC^GT[GA] 5640 TACGTG BsaWI 1648 ACCGGA [AT]^CCGG[AT] 3562 TCCGGT BsiEI 1443 CGGCCG CG[GA][CT]^CG 5497 CGGCCG BsmI 1596 GAATGC GAATGC(1/-1) 3006 GAATGC BspLU11I 1164 ACATGT A^CATGT 4937 ACATGT BssSI 1156 CACGAG CACGAG(-5/-1) 3011 CTCGTG BstBI 1220 TTCGAA TT^CGAA 2414 TTCGAA BstXI 3716 CCACCACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3729 CCAGACTGCTGG DraIII 243 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG 2672 CACCCAGTG EagI 1443 CGGCCG C^GGCCG 5497 CGGCCG EclXI 1443 CGGCCG C^GGCCG 5497 CGGCCG Eco52I 1443 CGGCCG C^GGCCG 5497 CGGCCG HindIII 2708 AAGCTT A^AGCTT 2918 AAGCTT NcoI 98 CCATGG C^CATGG 905 CCATGG NspV 1220 TTCGAA TT^CGAA 2414 TTCGAA PflFI 3808 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3844 GACAAGGTC PflMI 4410 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4734 CCACCCCCTGG SapI 541 GAAGAGC GCTCTTC(1/4) 1360 GAAGAGC SfiI 1104 GGCCTGTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 3682 GGCCTCCTCGGCC SfuI 1220 TTCGAA TT^CGAA 2414 TTCGAA Tsp45I 1976 GTGAC ^GT[GC]AC 2021 GTCAC Tth111I 3808 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3844 GACAAGGTC Van91I 4410 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4734 CCACCCCCTGG XbaI 993 TCTAGA T^CTAGA 5193 TCTAGA XmnI 1122 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5311 GAAGTGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AciI AfaI AflIII AluI Alw44I AlwI AlwNI AocI ApaI ApaLI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XcmI XhoII